apoE Expression in Glomerulus and Correlation with Glomerulosclerosis Induced by Adriamycin in Rats

Abstract

Lipid deposition in glomerulus plays an important role in the progression of glomerulosclerosis (GS), and apolipoprotein E (apoE) is an important protein in cholesterol homeostasis. This investigation was performed to explore whether there exists an association between apoE and GS susceptibility. Eighty Wistar rats were randomly divided into two groups: sham operation group and glomerulosclerosis model group, n = 40. The GS disease in rat was induced by uninephrectomy and injecting adriamycin (5 mg/kg) through the tail vein. At the end of 9 and 13 weeks, 20 rats in each group were killed and the relative samples were collected. Serum creatinine, blood urea nitrogen, and 24-h urine protein were determined. Immunohistochemical analysis was performed on renal tissue to detect the expression of apoE, collagen IV, fibronectin, a-smooth muscle actin, and transforming growth factor-b1 in glomerulus. Real-time reverse transcription polymerase chain reaction was conducted to detect the apoE mRNA expression in renal tissue. Compared with sham operation group at the end of 9/13 weeks, glomerulosclerosis model group exhibited levels of serum creatinine, blood urea nitrogen, 24-h urine protein, and a glomerulosclerosis index that were significantly elevated ( p < 0.01), and collagen IV, fibronectin, a-smooth muscle actin, and transforming growth factor-b1 protein expression and apoE expression (protein and mRNA) were significantly upregulated (p < 0.01). In conclusion, apoE can increase the accumulation of extracellular matrix in glomerulus and may take part in the progression of GS.

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

“…The absorbance was measured using an ultraviolet spectrophotometer, and agarose gel electrophoresis confirmed that there had been no degradation of RNA by visualizing the 18S and 28S RNA bands under ultraviolet light. 40,41 Primers were designed according to primer design principles by Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA). The primers for PAX2 and internal control β-actin were as follows: F 5 -AAGCGACAGAACCCGACTATGT-3 and R 5 -ACTCCTGTCCCTGCCCCAT-3 for PAX2; F 5 -GCCCCTGAGGAGCACCCTGT-3 and R 5 -ACGCTCGGTCAGGATCTTCA-3 for β-actin.…”

Effect of Ginkgo biloba extract on the expressions of Cox-2 and GST-Pi in rats with hepatocellular carcinoma risk

“…Ultraviolet spectrophotometer measuring absorbance, agarose gel electrophoresis confirmed that there had been no degradation of RNA by visualizing the 18S and 28S RNA bands under ultraviolet light [17,18]. Primers of were designed according to primer design principles by Primer Premier 5.0.…”

All-Trans Retinoic Acid Attenuates the Renal Interstitial Fibrosis Lesion in Rats but Not By Transforming Growth Factor-β1/Smad3 Signaling Pathway

“…Ultraviolet spectrophotometer measuring absorbance, agarose gel electrophoresis confirmed that there had been no degradation of RNA by visualizing the 18S and 28S RNA bands under ultraviolet light. 14,15 Primers were designed according to primer design principles by Primer Premier 5.0. The primers for Smad3 and internal control β-actin were as follows: F 5 0 -CTGGCTACCTGAGTGAAGATG-3 0 and R 5 0 -TGTGAAGCGTGGAATGTCTC-3 0 for Smad3; F 5 0 -GCCCCTGAGGAGCACCCTGT-3 0 and R 5 0 -ACGCTCGGTCAGGATCTTCA-3 0 for β-actin.…”