Apolipoprotein A-IV binds αIIbβ3 integrin and inhibits thrombosis

Xu, Xinyu; Wang, Yiming; Adili, Reheman; Ju, Lining Arnold; Spring, Christopher M.; Jin, Jiyu; Hong, Yang; Neves, Miguel A. D.; Chen, Pingguo; Yang, Yan; Xi, Lei; Chen, Yunfeng; Gallant, Reid C.; Xu, Miao; Zhang, Hailong; Song, Jina; Ke, Peifeng; Zhang, Dan; Carrim, Naadiya; Yu, Siyang; Zhu, Guangheng; She, Yi‐Min; Cyr, Terry D.; Fu, Wenbin; Liu, Guoqing; Connelly, Philip W.; Rand, Margaret L.; Adeli, Khosrow; Freedman, John; Lee, Jeffrey E.; Tso, Patrick; Marchese, Patrizia; Davidson, W. Sean; Jackson, Shaun P.; Zhu, Cheng; Ruggeri, Zaverio M.; Ni, Heyu

doi:10.1038/s41467-018-05806-0

Cited by 84 publications

(75 citation statements)

References 71 publications

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“…However, only three strains, namely Plank-SS-01, Lepto-CH and Osci-NB-01, contained a high percentage of oleic acid (25%, 28%, and 27%, respectively). Several studies on this monounsaturated acid have demonstrated its powerful antioxidant effect (Ceron et al, 2007) and its anti-platelets aggregation property (Xu et al, 2018), the acid thus presenting a promising potential as an accessible and safe alternative to synthetic molecules. In addition, Harada et al (2000) demonstrated the larvicidal activity of this fatty acid extracted from P. agardhii, especially against mosquito larvae.…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

Biochemical analysis of cyanobacterial strains isolated from different Tunisian inland waters: therapeutical and nutritional potential

Gharbi¹,

Fathalli²,

Fassatoui³

et al. 2019

Int. J. Bio. Chem. Sci

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The last decade period has been characterized by a remarkable increase in the need for food, bioenergy, and pharmaceutical compounds. Meanwhile, microalgae have been in the center of interest as a rich and sustainable source of highly valuable natural products and bioactive compounds. In this perspective, the present study aimed at investigating the biochemical composition of 10 cyanobacterial strains collected from several Tunisian inland waters, seeking to identify the possible use of cyanobacteria in nutraceuticals and pharmaceuticals industry, and for animal and human consumption. Ten local strains of cyanobacteria were isolated from several Tunisian inland waters and cultured in controlled laboratory conditions. Fatty Acid methyl esters were analyzed in each strain by Gas chromatography-Mass spectrometry (GC-MS) as well as total proteins, carbohydrates and different phycobiliproteins (phycocyanin, allophycocyanin and phycoerythrin). Results showed that Planktothrix agardhii, Leptolyngbya sp1., and Leptolyngbya sp2. contained 14 different fatty acids, among which oleic acid was the most abundant. These might be important in nutraceutical and agronomic sectors due to the potent antioxidant and larvicidal activities of oleic acid. Cylindrospermopsis raciborskii presents extraordinarily high levels of phycocyanin, making it an exceptional natural reservoir of effective and non-toxic drugs that are of particular interest against cancer and neurodegenerative diseases. Extracts from cyanobacterial strains represent a natural and available source of proteins, of highly valuable fatty acids, and especially of phycocyanin which is known by its extremely health beneficial properties. The nutritional and functional complements provided by these extracts can be used in the prevention of various diseases and also as effective and environmentally friendly insecticides.

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Section: Fatty Acid Compositionmentioning

confidence: 99%

Biochemical analysis of cyanobacterial strains isolated from different Tunisian inland waters: therapeutical and nutritional potential

Gharbi¹,

Fathalli²,

Fassatoui³

et al. 2019

Int. J. Bio. Chem. Sci

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“…Whole blood was incubated with testing reagents for 15 minutes at 37°C, and then perfused over immobilized collagen (100 μg/mL, overnight at 4°C) at flow rates that generated wall shear stresses of 5, 20 and 100 dynes/cm 2 for 5 minutes at room temperature in a microfluidic chamber (Cellix Mirus Nanopump), according to a previously established protocol . The chamber was then washed with phosphate‐buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

Physical proximity and functional cooperation of glycoprotein 130 and glycoprotein VI in platelet membrane lipid rafts

Houck

Yuan

Tian

et al. 2019

Journal of Thrombosis and Haemostasis

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Objective:Clinical and laboratory studies have demonstrated that platelets become hyperactive and prothrombotic in conditions of inflammation. We have previously shown that the proinflammatory cytokine interleukin (IL)-6 forms a complex with soluble IL-6 receptor α (sIL-6Rα) to prime platelets for activation by subthreshold concentrations of collagen. Upon being stimulated with collagen, the transcription factor signal transducer and activator of transcription (STAT) 3 in platelets is phosphorylated and dimerized to act as a protein scaffold to facilitate the catalytic action between the kinase Syk and the substrate phospholipase Cγ2 (PLCγ2) in collagen-induced signaling. However, it remains unknown how collagen induces phosphorylation and dimerization of STAT3. Methods and results:We conducted complementary in vitro experiments to show that the IL-6 receptor subunit glycoprotein 130 (GP130) was in physical proximity to the collagen receptor glycoprotein VI (GPVI in membrane lipid rafts of platelets.This proximity allows collagen to induce STAT3 activation and dimerization, and the IL-6-sIL-6Rα complex to activate the kinase Syk and the substrate PLCγ2 in the GPVI signal pathway, resulting in an enhanced platelet response to collagen. Disrupting lipid rafts or blocking GP130-Janus tyrosine kinase (JAK)-STAT3 signaling abolished the cross-activation and reduced platelet reactivity to collagen.Conclusion: These results demonstrate cross-talk between collagen and IL-6 signal pathways. This cross-talk could potentially provide a novel mechanism for inflammation-induced platelet hyperactivity, so the IL-6-GP130-JAK-STAT3 pathway has been identified as a potential target to block this hyperactivity. K E Y W O R D Scytokine receptor GP130, membrane microdomain, platelet activation, platelet membrane glycoproteins, STAT3 transcription factor | 1501 HOUCK et al.

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“…In terms of coagulation-related indicators, most scientists believe that inflammation can stimulate the vascular endothelium to activate platelets and promote the binding of platelets to fibrinogen, thereby promoting arteriosclerotic thrombosis. Our previous study revealed that apolipoprotein A-IV is a new ligand for platelet GPIIβ/IIIα integrin, which competitively inhibits the binding of glycoprotein GPIIβ/IIIα to fibrinogen and inhibits platelet aggregation [25,27]. In this study, platelet function will be used as an entry point to explore whether acupuncture can antagonize the binding of platelets to fibrinogen by changing the level of apolipoprotein A-IV, thereby reducing the PAR in blood and reducing the formation of plaque.…”

Section: Comprehensive Selection Of Outcome Measuresmentioning

confidence: 99%

Acupuncture treatment for carotid atherosclerotic plaques: protocol for a randomized, blind clinical trial

Zhou

Zhao

Meng³

et al. 2020

Preprint

Self Cite

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Background: Carotid atherosclerosis disease (CAD) is generally associated with the occurrence of cardiovascular and cerebrovascular accidents. However, CAD has not been taken seriously enough in the clinic, which, coupled with the single treatment and prevention of CAD, has led to a generally low level of patient compliance. Therefore, acupuncture is expected to be a safe and effective therapy that can be maintained in the long term for patients with CAD. Methods: This is a three-arm randomized clinical trial. Three groups (TA, SA and MC) will be randomly allocated at a 1:1:1 ratio. The study will enrol 105 cervical atherosclerosis plaque patients in total on a voluntary basis, with 35 patients in each group. The treatment will last for 12 weeks with 2 treatments per week for 24 treatments in total. Results: Two 3D ultrasound indicators will be measured as the primary outcomes: the total plaque volume (PV) of the carotid artery on each side and the grey-scale median (GSM). The secondary outcomes will include intima-media thickness (IMT), lipid levels, apolipoprotein A-IV level, platelet count (PLT), fibrinogen (FIB) and platelet aggregation rate (PAR). All the outcomes will be assessed before treatment, after treatment and after a 12-week follow-up period. This study will utilize per-protocol (PP) and intention-to-treat (ITT) analysis principles. Conclusions: This trail is to evaluate the efficacy and reliability of acupuncture in relieving carotid atherosclerotic plaques by establishing acupuncture (TA), sham-acupuncture (SA) and medication (MC) groups.

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Apolipoprotein A-IV binds αIIbβ3 integrin and inhibits thrombosis

Cited by 84 publications

References 71 publications

Biochemical analysis of cyanobacterial strains isolated from different Tunisian inland waters: therapeutical and nutritional potential

Biochemical analysis of cyanobacterial strains isolated from different Tunisian inland waters: therapeutical and nutritional potential

Physical proximity and functional cooperation of glycoprotein 130 and glycoprotein VI in platelet membrane lipid rafts

Acupuncture treatment for carotid atherosclerotic plaques: protocol for a randomized, blind clinical trial

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