Apolipoprotein A1 −75 G/A and +83 C/T polymorphisms: susceptibility and prognostic implications in breast cancer

Abstract

Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. In the current report, we evaluated the role of the functional ApoA1 polymorphisms (-75 G/A and +83 C/T) as genetic markers for breast cancer susceptibility and prognosis. We used the polymerase chain reaction and restriction enzyme digestion (RFLP-PCR) to characterize the variations of the ApoA1 gene in 295 unrelated Tunisian patients with breast carcinoma and 197 healthy control subjects. No association was found between the +83 C/T genetic variation in ApoA1 gene and the risk of breast cancer occurrence. The presence of the (+83) T allele appeared however to be associated with an increased risk of lymph node metastasis occurrence (OR = 2.94; P = 0.01). Furthermore, a positive association was found between ApoA1 -75 A allele carriers and breast cancer risk (OR = 1.57; P = 0.02). Regarding prognostic indicators, a significant association was found between ApoA1 (-75) A allele carriers and the premenopausal status of breast cancer patients (OR = 1.73; P = 0.03). Additionally, the presence of the -75 A allele was correlated with the oestrogen receptor status among premenopausal women (OR = 2.45; P = 0.02). This is the first report on the studies of ApoA1 single nucleotide polymorphisms (SNPs) in breast carcinomas. Our data suggest that these genetic variations of ApoA1 may represent a marker for the increased risk of breast cancer.

Identification of ApoA1, HPX and POTEE genes by omic analysis in breast cancer

“…As confirmed by several studies, Apo-A1 suppresses inflammation, tumor growth, angiogenesis, invasion and metastasis (22). Studies have found that Apo-A1 is a potential biomarker for many types of cancer including breast and pancreatic carcinomas (23)(24)(25)(26)(27). It is usually thought that the role of the Apo-A1 molecule in cancer pathophysiology is associated with the phospholipid binding ability.…”

The dyslipidemia-associated SNP on the APOA1/C3/A5 gene cluster predicts post-surgery poor outcome in Taiwanese breast cancer patients: a 10-year follow-up study

“…Tunisian) and age differences (62.7% vs . 38.9% <50years) [34]. Nevertheless, in our longitudinal study we found that APOA1 rs670 predicted worse outcome after adjustment for ER/PR status, indicating alternative contributions from APOA1 rs670 in cancer progression.…”

“…A cross-sectional study focusing on the association of APOA1 SNPs with breast cancer and patient phenotype reported that the -75G/A polymorphism correlated with breast cancer risk at baseline [34]. However, the slight discrepancy in associations of -75G/A polymorphism with ER status observed by us and Hamrita et al is likely due to ethnic (Taiwanese vs .…”

Identifying Grade/Stage-Related Active Modules in Human Co-regulatory Networks: A Case Study for Breast Cancer

“…Not only RARB, but also RARA, RARG, and RXRA might serve as potential targets in breast cancer prevention and therapy trials (Klaholz et al, 2000). Their target genes, such as APOA1, the major apoprotein constituent of highdensity lipoprotein, also play a major role in the invasion and metastasis of breast cancer (Hamrita et al, 2010;Han et al, 2008). Metastasis is the main feature of tumor grade, and indicates the malignant spread of the tumor.…”