Apolipoprotein E determines the hepatic transcriptional profile of dietary maslinic acid in mice

Guillén, Natalía; Acı́n, Sergio; Surra, Joaquín C.; Arnal, Carmen; Godino, Javier; Garcı́a-Granados, Andrés; Muniesa, Pedro; Ruı́z-Gutı́errez, Valentina; Osada, Jesús

doi:10.1016/j.jnutbio.2008.08.003

Cited by 16 publications

(13 citation statements)

References 47 publications

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“….). This strategy had been proved really valuable in our hands (15,16) and indicates that pooled samples of histologically comparable tissues can successfully be used to provide an initial screening of gene expression in studies of this type, with the attending economic and time savings, although with the limitation of no information on biological variability.…”

Section: Resultsmentioning

confidence: 99%

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites

Nuño-Ayala

Guillén

Arnal

et al. 2012

Physiological Genomics

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mocysteinemia has been reported in human reproduction as a risk factor for early pregnancy loss, preeclampsia, and congenital birth defects like spina bifida. Female infertility was also observed in cystathionine beta synthase-deficient mice (Cbs-KO) as an animal model for severe hyperhomocysteinemia. The aim for the present research was to elucidate the time-point of pregnancy loss and to pinpoint gene and cellular changes involved in the underlying pathological mechanism. By mating 90-dayold wild-type and Cbs-KO female mice with their homologous male partners, we found that pregnancy loss in Cbs-KO occurred between the 8th and 12th gestation day during placenta formation. DNA microarrays were carried out on uterus from implantation and interimplantation samples obtained on day 8. The results allowed us to select genes potentially involved in embryo death; these were individually confirmed by RT-qPCR, and their expressions were also followed throughout pregnancy. We found that changes in expression of Calb1, Ttr, Expi, Inmt, Spink3, Rpgrip1, Krt15, Gzmc, Gzmb, Tdo2, and Afp were important for pregnancy success, since a different regulation in Cbs-KO mice was found. Also, differences in relationships among selected genes were observed, indicating a dysregulation of these genes in Cbs-KO females. In conclusion, our data provide more information on the gene expression cascade and its timely regulated process required for a successful pregnancy. In addition, we unveil new potential avenues to explore further investigations in pregnancy loss.reproduction; hyperhomocysteinemia; uterus IN EPIDEMIOLOGICAL AND META-ANALYSIS studies, hyperhomocysteinemia (Hhcy) has been found to be a risk factor for placentally mediated diseases, such as preeclampsia, spontaneous abortion, and placental abruption (50). In case-control studies, it has been also reported that homocysteine (Hcy) levels are frequently elevated in complicated pregnancies due to preeclampsia or umbilical placental vascular disease over those present in the normal pregnancy group (11,37,54).Hcy is a nonprotein sulfur amino acid and a substrate for cystathionine ␤-synthase (Cbs) in the transsulfuration pathway, which represents its main metabolic fate. Alternatively, Hcy can be converted into methionine through the remethylation pathway. Three metabolic reactions using different methyl donors are known; one uses betaine, and the reaction is catalyzed by betaine-homocysteine methyltransferase, the second one catalyzed by 5-methyltetrahydrofolate-homocysteine methyltransferase uses as donor 5-methyltetrahydrofolate (43), and the third one is catalyzed by S-methylmethionine-homocysteine methyltransferase with methyl donor S-methylmethionine (46). These metabolic pathways are expressed in different tissues during development. Thus, Cbs is expressed in liver, pregnant myometrium, and decidual tissue, whereas methionine synthase is expressed in all embryonic tissues, the liver being the primary site of activity for these enzymes (48,51,58).Cbs-deficient [Cbs knockout...

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Section: Resultsmentioning

confidence: 99%

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites

Nuño-Ayala

Guillén

Arnal

et al. 2012

Physiological Genomics

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“…Dbp was upregulated to an extent depending on the genetic background of the mice and was negatively associated with the expression of Marco . Thus, maslinic acid is active in controlling hepatic gene expression and some of its effects are modulated by the presence ofAPOE [42]. The other triterpene, oleanolic acid, administered to Apoe -deficient mice, increased the hepatic area occupied by lipid droplets with no change in oxidative stress.…”

Section: Transcriptional Analysis Of Mediterranean Diet Consumptiomentioning

confidence: 99%

Transcriptomics and the Mediterranean Diet: A Systematic Review

et al. 2017

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The Mediterranean diet has been proven to be highly effective in the prevention of cardiovascular diseases and cancer and in decreasing overall mortality. Nowadays, transcriptomics is gaining particular relevance due to the existence of non-coding RNAs capable of regulating many biological processes. The present work describes a systematic review of current evidence supporting the influence of the Mediterranean diet on transcriptomes of different tissues in various experimental models. While information on regulatory RNA is very limited, they seem to contribute to the effect. Special attention has been given to the oily matrix of virgin olive oil. In this regard, monounsaturated fatty acid-rich diets prevented the expression of inflammatory genes in different tissues, an action also observed after the administration of olive oil phenolic compounds. Among these, tyrosol, hydroxytyrosol, and secoiridoids have been found to be particularly effective in cell cycle expression. Less explored terpenes, such as oleanolic acid, are important modulators of circadian clock genes. The wide range of studied tissues and organisms indicate that response to these compounds is universal and poses an important level of complexity considering the different genes expressed in each tissue and the number of different tissues in an organism.

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“…When especifically maslinic acid-enriched oils were tested, Cyp2b9 , Cyp2b13 , and Dbp expressions appeared significantly increased, and Marco was significantly decreased in apoE-deficient mice. Dbp was upregulated to an extent depending on the genetic background of the mice and negatively associated with the expression of Marco , a gene strongly upregulated by the absence of apoE [130]. Secoiridoids are also minor components of extra virgin olive oil belonging to aqueous soluble category.…”

Section: Minor Dietary Components Of Foodsmentioning

confidence: 99%

The Use of Transcriptomics to Unveil the Role of Nutrients in Mammalian Liver

Osada

2013

ISRN Nutrition

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Liver is the organ primarily responding to diet, and it is crucial in determining plasma carbohydrate, protein, and lipid levels. In addition, it is mainly responsible for transformation of xenobiotics. For these reasons, it has been a target of transcriptomic analyses. In this review, we have covered the works dealing with the response of mammalian liver to different nutritional stimuli such as fasting/feeding, caloric restriction, dietary carbohydrate, cholesterol, fat, protein, bile acid, salt, vitamin, and oligoelement contents. Quality of fats or proteins has been equally addressed, and has the influence of minor dietary components. Other compounds, not purely nutritional as those represented by alcohol and food additives, have been included due to their relevance in processed food. The influence has been studied not only on mRNA but also on miRNA. The wide scope of the technology clearly reflects that any simple intervention has profound changes in many metabolic parameters and that there is a synergy in response when more compounds are included in the intervention. Standardized arrays to systematically test the same genes in all studies and analyzing data to establish patterns of response are required, particularly for RNA sequencing. Moreover, RNA is a valuable, easy-screening ally but always requires further confirmation.

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Apolipoprotein E determines the hepatic transcriptional profile of dietary maslinic acid in mice

Cited by 16 publications

References 47 publications

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites

Cystathionine β-synthase deficiency causes infertility by impairing decidualization and gene expression networks in uterus implantation sites

Transcriptomics and the Mediterranean Diet: A Systematic Review

The Use of Transcriptomics to Unveil the Role of Nutrients in Mammalian Liver

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