Apolipoprotein E Stimulates Bone Formation on Titanium in Vitro

Kodama, Takashi; Goto, Tetsuya; Ishibe, T; Kobayashi, Shigeru; Takahashi, Tsuneo

doi:10.1016/s0915-6992(07)80023-9

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Another interesting property of OCP reported so far is the capacity adsorbing serum derived proteins, such as α2HS-glycoporteins [ 83 ], apolipoprotein E (Apo E) and complement 3 (C3) [ 84 ] on the crystal surface and also within the space between the crystals in OCP granules. Apo E and C3 have been reported to have functions in osteoblast differentiation [ 85 ] and in osteoclastic cells recruitment [ 86 ], respectively, suggesting that OCP has a positive role not only for host bone-related cells but also for osteoblastic and osteoclastic cells associated with autologous bone through these adsorbed proteins. Other studies have shown that soluble amorphous calcium phosphate (ACP) [ 87 ], reactive calcium phosphate cements [ 88 ] and mineralized HA crystals together with magnesium oxide (MgO) [ 89 ] display significant effects on increasing osteogenic cellular activities.…”

Section: Discussionmentioning

confidence: 99%

Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

Ozaki

Hamai

Sakai

et al. 2021

Science and Technology of Advanced Materials

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This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were implanted in rat CSD for 8 weeks, and subjected to X-ray micro-computed tomography (micro-CT), histology, tartrate-resistant acid phosphatase (TRAP) staining, and histomorphometry for bone regeneration. Osteoblastic differentiation from mesenchymal stem cells (D1 cells) was examined in the presence of non-contacting materials by alkaline phosphatase (ALP) activity for 21 days. The material properties and medium composition before and after the incubation were determined by selected area electron diffraction (SAED) under transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and chemical analysis. The results showed that while bone formation coupled with TRAP-positive osteoclastic resorption and cellular ALP activity were the highest in the Auto group, a positive effect per OCP weight or per autologous bone weight on ALP activity was found. Although the OCP structure was maintained even after the incubation (SAED), micro-deposits were grown on OCP surfaces (TEM). Fibrous tissue was also exposed on the autologous bone surfaces (SEM). Through FT-IR absorption, it was determined that bone mineral-like characteristics of the phosphate group increased in the OCP + Auto group. These findings were interpreted as a structural change from OCP to the apatitic phase, a conclusion supported by the medium degree of saturation changes. The results demonstrate the mutual chemical effect of mixing OCP with autologous bone as an active bone substitute material.

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Section: Discussionmentioning

confidence: 99%

Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

Ozaki

Hamai

Sakai

et al. 2021

Science and Technology of Advanced Materials

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“…Two bone formation-related proteins were quantitatively identified: one is apolipoprotein E (Apo E) and the other is complement 3 (C3). Apo E and C3 are known to be involved in promoting osteoblast differentiation [27] and recruiting mononuclear osteoclasts [28]. It is probable that OCP incubated in vitro and implanted in vivo may work a better adsorbent for such a protein involved in bone formation [26].…”

Section: Ocp As a Potential Carrier For Cellsmentioning

confidence: 99%

Octacalcium Phosphate: A Potential Scaffold Material for Controlling Activity of Bone-Related Cells <i>In Vitro</i>

Сузукі

Anada

2014

MSF

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We have previously established a wet synthesis method of octacalcium phosphate (OCP) in a relatively large scale and found that OCP enhances bone formation more than synthetic hydroxyapatite (HA) if implanted onto bone surface and various bone defects. The present paper reviews, based on our studies, as to how OCP controls in vitro cellular activities of bone-related cells, such as bone marrow stromal cells, and how OCP enhances bone repair in critical sized bone defect experimentally created in animal models. OCP tends to progressively convert to HA in culture media and in rat calvaria defects. OCP is capable of enhancing in vitro osteoblast differentiation and osteoclast formation in the presence of osteoblasts. Recent our studies also indicated that OCP enhances odontoblast differentiation while suppresses chondrogenic differentiation. The physicochemical properties, such as chemical composition and adsorption affinity of serum proteins, vary depending on the advancement of conversion from OCP to HA, which suggests that the change on the surface property during the conversion of OCP may affect the cellular responses in vitro and tissue reaction in vivo. OCP could be used as a scaffold material that can control the activity of bone-related cells.

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Evolution of octacalcium phosphate biomaterials

Сузукі

2020

Octacalcium Phosphate Biomaterials

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Apolipoprotein E Stimulates Bone Formation on Titanium in Vitro

Cited by 8 publications

References 17 publications

Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration

Octacalcium Phosphate: A Potential Scaffold Material for Controlling Activity of Bone-Related Cells <i>In Vitro</i>

Evolution of octacalcium phosphate biomaterials

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