Apolipoproteins AI/B/E gene polymorphism and their plasma levels in patients with coronary artery disease in a tertiary care-center of Eastern India

Biswas, Santanu; Ghoshal, Pradip K.; Halder, Bhubaneswar; Ganguly, Kajal; Dasbiswas, Arup; Mandal, Nripendranath

doi:10.1016/j.ihj.2013.10.003

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…In contrast to the studies by Bora et al (13), Kamboh et al (15), Dawar et al,and Biswas et al (19,34), the homozygous TT (-) genotype was observed in our study. The genotype has been observed only in a few studies, such as those by Sigurdsson et al and Heng et al (35,36), and accounts for less than 1 percent of the population.…”

Section: Discussioncontrasting

confidence: 99%

Association Between MspI Polymorphisms of the Apolipoprotein A-I Gene and Hyperlipidemia in an Iranian Population

Pakdel

Eidgahi

Bandegi

2018

Middle East J Rehabil Health Stud

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Background: One of the most important metabolic disorders is hyperlipidemia. ApoAI protein plays an important role in lipoprotein metabolism. In this study, the association between MspI polymorphisms in the promoter (G-75A) and first intron (C83T) of the apoAI gene and hyperlipidemia was investigated in Semnan, Iran. Methods: A total of 151 unrelated subjects were divided into two groups: the hyperlipidemic (N = 75) and control (N = 76) groups. Genotyping was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: In the hyperlipidemic group, the frequency of the (+-) variant of the MspI (C83T) locus was higher than that in the control group (P < 0.05). Significant differences were found in serum HDL-C and apoAI levels between different genotypes of MspI (C83T) (P < 0.001 and P = 0.02, respectively). In the hyperlipidemic group, the odds ratios for the (+-) and (-) genotypes of the MspI (C83T) locus in comparison to that for the (++) genotype were 0.26 (P= 0.023) and 3.62 (P = 0.254), respectively. The allelic frequency at the (G-75A) locus was not significantly different in the hyperlipidemic and control groups (P = 0.36). The serum levels of lipid and lipoprotein were not significantly different for all genotypes of MspI (G-75A). The AA/++ and GG/-haplotypes had the lowest and

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Section: Discussioncontrasting

confidence: 99%

Association Between MspI Polymorphisms of the Apolipoprotein A-I Gene and Hyperlipidemia in an Iranian Population

Pakdel

Eidgahi

Bandegi

2018

Middle East J Rehabil Health Stud

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“…The homozygous TT genotype was not detected in any of the study subjects. The rarity of the TT genotype seems to be a universal phenomenon as it was not detected in a number of other populations too ( Kamboh et al, 1996 , Ma et al, 2003 , Jia et al, 2005 , Dawar et al, 2010 , Biswas et al, 2013 , Padmaja et al, 2009 ). When detected, the frequency of this variant was mostly found to be less than 1% ( Sigurdsson et al, 1992 , Heng et al, 2001 , Larson et al, 2002 , Miroshnikova et al, 2011 , Hamrita et al, 2011 ).…”

Section: Discussionmentioning

confidence: 96%

“…Historically, the G-75A and the C+83T polymorphisms have been investigated in relation to coronary artery disease (CAD) and cardiovascular risk factors ( Reguero et al, 1998 , Jeenah et al, 1990 , Sigurdsson et al, 1992 , Talmud et al, 1994 , Wang et al, 1996 , Bai et al, 1996 , Chhabra et al, 2003 , Shanker et al, 2008 , Dawar et al, 2010 , Miroshnikova et al, 2011 , Biswas et al, 2013 ). However, it is increasingly believed that these polymorphisms may have important roles to play in a host of other disorders such as Alzheimer's disease ( Vollbach et al, 2005 , Smach et al, 2011 ), multiple sclerosis ( Koutsis et al, 2009 ), schizophrenia ( Yang et al, 2010 ), breast cancer ( Hamrita et al, 2011 ), Parkinson's disease ( Swanson et al, 2014 ), cholelithiasis ( Dixit et al, 2007 ), gout ( Cardona et al, 2005 ), diabetes mellitus ( Ma et al, 2003 ) and acute lung injury following cardio-pulmonary bypass surgery ( Tu et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Single nucleotide polymorphisms of APOA1 gene and their relationship with serum apolipoprotein A-I concentrations in the native population of Assam

Bora

Pathak

Borah³

et al. 2016

Meta Gene

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BackgroundThere is a growing interest in the role of allelic variants of the APOA1 gene in relation to a number of disorders. We described two common polymorphisms of the APOA1 gene, G-75A and C+83T and investigated their potential influence on the serum apolipoprotein A-I (apo A-I) levels in the native population of Assam — a region that is ethnically distinct and from where no information is hitherto available.MethodsBlood samples were collected from 150 healthy volunteers. Apo A-I levels were estimated by immunoturbidometry. Genotyping was done by a PCR-RFLP method that involved DNA extraction from whole blood, followed by polymerase chain reaction and digestion of the PCR product by MspI restriction enzyme, and analysis of fragment sizes in 12% polyacrylamide gel.ResultsThe GG variant at G-75A locus and CC variant at C+83T locus were the most prevalent. GG/CC was the most common combination. Homozygous TT genotype was not detected in any of the subjects. The rare allele frequencies for the G-75A and C+83T sites were found to be 0.22 and 0.06 respectively, which significantly differed from those reported in some other populations in neighbouring regions. Serum apo A-I concentrations did not vary significantly across the detected genotypes. These findings were consistent in both sexes.ConclusionWe described the distribution of the G-75A and C+83T polymorphisms of the APOA1 gene in the population of Assam for the first time. These polymorphisms were not found to directly influence apo A-I concentrations in this population either individually or synergistically.

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“…lipidaccumulation and also accumulation of cells involved in the process of inflammation [Jabir et al, 2016b;Neelofar et al, 2016]. Despite the different demographic locations and lifestyle profiles of individuals all across the world, continuous rise in CAD cases have been reported in the scientific literature which suggests an important role of genetic factor(s) [Biswas et al, 2013]. Growing evidence also indicates the critical involvement of several proinflammatory cytokines in the CAD pathogenesis [Kofler et al, 2005;Briasoulis et al, 2016;Yin et al, 2017].…”

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confidence: 99%

Estimation of Interleukin-1β Promoter (−31 C/T and −511 T/C) Polymorphisms and Its Level in Coronary Artery Disease Patients

et al. 2017

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Interleukin-1β (IL-1β) is an inflammation-causing cytokine that exerts several unique biological effects and could lead to future adverse events of CAD. The piece of work presented herein is aimed at investigating possible association of IL-1β levels to its polymorphic site viz. -511 and -31 at promoter region in Saudi CAD patients. The study included 155 confirmed CAD patients and 80 healthy control individuals both men and women. Concentration of IL-1β in the patients' serum was measured by ELISA method. For single nucleotide polymorphism (SNP) analysis, sanger method of DNA sequencing was followed. We observed variable numbers of SNPs at -31 C/T and -511 T/C promoter regions in Saudi patients suffering from CAD in comparison to the control set of individuals. However, the changes in the number of SNP-hotspots were determined to be non-significant with reference to the control set. The haplotype analysis at -31 and -511 also did not show any significant changes between control and CAD patients. Moreover, serum IL-1β levels were observed to be expressively higher in patients suffering from CAD (P < 0.001) and its associated complications viz. STEMI (P < 0.001), NSTEMI (P < 0.001), and UA (P < 0.001). Our study provides the status of SNPs at IL-1β promoter in Saudi population. As per our information, ours is the first article that shows the genetic diversity in IL-1β promoters and its level in the Saudi CAD patients. J. Cell. Biochem. 118: 2977-2982, 2017. © 2017 Wiley Periodicals, Inc.

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Apolipoproteins AI/B/E gene polymorphism and their plasma levels in patients with coronary artery disease in a tertiary care-center of Eastern India

Abstract: Our findings suggest that the polymorphisms apo AI MspI and apo B EcoRI do not seem to affect CAD. But the genotype with E4 allele of apo E gene independent of other risk factors is associated with this disease.

Cited by 9 publications

References 34 publications

Association Between MspI Polymorphisms of the Apolipoprotein A-I Gene and Hyperlipidemia in an Iranian Population

Association Between MspI Polymorphisms of the Apolipoprotein A-I Gene and Hyperlipidemia in an Iranian Population

Single nucleotide polymorphisms of APOA1 gene and their relationship with serum apolipoprotein A-I concentrations in the native population of Assam

Estimation of Interleukin-1β Promoter (−31 C/T and −511 T/C) Polymorphisms and Its Level in Coronary Artery Disease Patients

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