Apomixis in Eulaliopsis binata: characterization of reproductive mode and endosperm development

Yao, Jialing; Zhou, Yun; Hu, Chun-Gen

doi:10.1007/s00497-007-0051-y

Cited by 28 publications

(19 citation statements)

References 21 publications

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“…Quantitative estimation of such embryos in progeny of apomictic plants was done to know the extent of uncoupling between components (Matzk et al 2001;Weiners et al 2006;Yao et al 2007). Theoretically, if all the three components are allowed to recombine freely, will yield to eight pathways (2 3 ) of seed development.…”

Section: Discussionmentioning

confidence: 99%

Reproductive pathways of seed development in apomictic guinea grass (Panicum maximum Jacq.) reveal uncoupling of apomixis components

et al. 2008

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One hundred and sixty accessions representing global germplasm of guinea grass (Panicum maximum Jacq.), an important apomictic (aposporous) fodder crop, were subjected to study on reproductive diversity in apomictic seed development utilizing ovule clearing and Xow cytometric seed screen (FCSS). Single seed FCSS of selected 14 tetraploid and Wve hexaploid lines demonstrated uncoupling between the three apomixis components, viz. apomeiosis, parthenogenesis and functional endosperm development, in natural as well as experimental populations, though it diVered across ploidy levels and genotypes. Reconstruction of reproductive pathways yielded a total of eight diVerent pathways of seed development, arising by uncoupling/recombination between apomixis components. Amongst these, two pathways involving modiWcations in embryo-sac (ES) (presence of two polar nuclei in aposporous ES that fuse prior to fertilization) and fertilization process (fusion of only one polar nucleus in a sexual ES) have been reported for the Wrst time. Some of the combinations, such as M I (haploids arising from parthenogenetic development of reduced egg cell), were found viable only in hexaploid background.Germplasm lines with higher expression of individual components were also identiWed. These components (including autonomous endosperm development) were also experimentally partitioned in hexaploid progenies (derived from a tetraploid parent viz. accession IG 04-164) that showed segregation in their reproductive capacities, and are reported for the Wrst time. Occurrence of several apomixis recombinants (phenotypic) in guinea grass lines suggested their hybrid origin, favors a multigene model for apomixis, with their penetrance aVected by modiWers and epigenetic mechanisms, in contrast to earlier reports of single locus control. Implications of partitioning components on apomixis research are discussed.

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Section: Discussionmentioning

confidence: 99%

Reproductive pathways of seed development in apomictic guinea grass (Panicum maximum Jacq.) reveal uncoupling of apomixis components

et al. 2008

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“…The shoots were separated into segments with one node, fixed, stored in FAA, and grouped according to their bud location. Paraffin section analysis followed the method of Yao et al (2007).…”

Section: Plant Materials and Sample Collectionsmentioning

confidence: 99%

PtSVP, an SVP homolog from trifoliate orange (Poncirus trifoliata L. Raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic Arabidopsis and tobacco plants

Zhang

Mei

et al. 2010

Plant Mol Biol

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A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud differentiation, PtSVP was expressed intensively in dormant tissue and vegetative meristems. PtSVP transcripts were detected in apical meristems before floral transition, then down-regulated during the transition. PtSVP expression was higher in differentiated (flower primordium) than in undifferentiated cells (apical meristems). The PtSVP expression pattern during apical meristem determination suggested that its function is not to depress flower initiation but to maintain meristem development. Transcription of PtSVP in Arabidopsis svp-41 showed partially rescued SVP function. Ectopic overexpression of PtSVP in wild-type Arabidopsis induced late flowering similar to the phenotypes induced by other SVP/StMADS-11-like genes, but transformants produced additional trichomes and floral defects, such as flower-like structures instead of carpels. Ectopic expression of PtSVP in tobacco also caused additional florets. Overexpression of PtSVP in tobacco inhibited early transition of the coflorescence and prolonged coflorescence development, thus causing additional florets at the later stage. A yeast two-hybrid assay indicated that PtSVP significantly interacted with PtAP1, a homolog of Arabidopsis APETALA1 (AP1). These findings suggest that citrus SVP homolog genes are involved in flowering time regulation and may influence inflorescence meristem identity in some conditions or genetic backgrounds. SVP homologs might have evolved among plant species, but the protein functions are conserved between Arabidopsis and citrus.

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“…We also observed, in H. monacanthus and H. megalanthus, several cases of embryos developing in the chalazal region. This phenomenon has also been reported in a nonflowering seed plant, Gnetum gnemon (Carmichael and Friedman, 1995) and in the apomict Eulaliopsis binata (Yao et al, 2007).…”

Section: Discussionmentioning

confidence: 78%

Ovule morphology, embryogenesis and seed development in three Hylocereus species (Cactaceae)

Cisneros¹,

Garcia²,

Tel-Zur³

2011

Flora

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Apomixis in Eulaliopsis binata: characterization of reproductive mode and endosperm development

Cited by 28 publications

References 21 publications

Reproductive pathways of seed development in apomictic guinea grass (Panicum maximum Jacq.) reveal uncoupling of apomixis components

Reproductive pathways of seed development in apomictic guinea grass (Panicum maximum Jacq.) reveal uncoupling of apomixis components

PtSVP, an SVP homolog from trifoliate orange (Poncirus trifoliata L. Raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic Arabidopsis and tobacco plants

Ovule morphology, embryogenesis and seed development in three Hylocereus species (Cactaceae)

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