Apoptosis and p53 expression in the placental villi of females with unexplained recurrent spontaneous abortion

Dehua, Wei; Wu, Qinghua; Shi, Huirong

doi:10.3892/etm.2013.1399

Cited by 25 publications

(23 citation statements)

References 17 publications

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“…To explore the roles of p53 during the pathogenesis of RM, we first detected the p53 protein level in the villous tissues from 27 RM patients and paired healthy controls. We observed overexpressed p53 in the RM patients, which is in accordance with results of other researchers (Figure A,B) . Recently, lncRNAs have been identified to function as key regulators of diverse cellular processes including pregnancy .…”

Section: Resultssupporting

confidence: 92%

“…Several single nucleotides polymorphisms were found to contribute to the high risk of RM . Meanwhile, p53 has been observed to be expressed abnormally in the chorionic villi and decidua of females with hydropic, spontaneous or missed abortions, and high p53 level in the chorionic villi from patients with RM was also observed, indicating its important roles during the pathogenesis of RM …”

Section: Introductionmentioning

confidence: 99%

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Disordered p53‐MALAT1 pathway is associated with recurrent miscarriage

Wang

Liu

et al. 2019

The Kaohsiung J of Med Scie

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Recurrent miscarriage (RM) affects about 1% of couples; however, the etiologies of half of the cases remain unknown. P53, a negative cell cycle regulator, has been found to modulate the expression of several long noncoding RNAs (lncRNAs) and overexpressed p53 has been observed in RM patients. To investigate the relationship between p53 and lncRNAs in the pathogenesis of RM, we detected the expression of p53 and six candidate lncRNAs in the villous from 27 RM patients and paired healthy controls. We found the level of NEAT1 and MALAT1 was reduced significantly and only the MALAT1 level negatively correlated with p53 protein level. By luciferase assay, we confirmed that p53 repress MALAT1 expression through directly binding to the promoter region. Functional study by using human trophoblast cell HTR‐8/SVneo, we observed that p53 overexpression lead to decreased cells proliferation, migration, invasion and increased apoptosis. Meanwhile, MALAT1 overexpression partially restored these function of p53 overexpression.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Disordered p53‐MALAT1 pathway is associated with recurrent miscarriage

Wang

Liu

et al. 2019

The Kaohsiung J of Med Scie

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“…In this study, we found MDP stimulation of NOD2 in DSCs significantly increased their apoptotic rate, which may be a contributing factor in spontaneous abortion [43]–[45]. However, further investigation is required to clarify the effect of MDP-induced apoptosis in DSCs on pregnancy outcome.…”

Section: Discussionmentioning

confidence: 63%

Expression and Functional Characterization of NOD2 in Decidual Stromal Cells Isolated during the First Trimester of Pregnancy

et al. 2014

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NOD2, one of the cytosolic proteins that contain a nuclear oligomerization domain (NOD), is a pattern recognition receptor (PRR) involved in innate immune responses to intracellular pathogens. Little is known, however, about the effect of NOD2 expression on the maternal–fetal relationship. Our aim was to elucidate the functions of NOD2 in normal decidual stromal cells (DSCs) from the first trimester. Tissues and DSCs were isolated from 26 patients with normal pregnancies that required abortion. The expression of NOD2 in deciduas/decidual stromal cells (DSCs) was examined by real-time PCR, immunohistochemistry, and In-cell western. DSCs containing NOD2 were stimulated by its ligand, muramyl dipeptide (MDP). The secretion of various cytokines and chemokines were measured by ELISA and the apoptotic rate was determined by flow cytometry. Treatment with MDP significantly elevated the expression of both NOD2 mRNA and protein levels in DSCs. In addition, MDP activation of NOD2 significantly increased IL-1β and MCP-1 cytokine expression in a dose dependent manner but had no effect on IL-12 expression. IL-1β and TNF-α also significantly increased the expression of NOD2 in DSCs, suggesting a positive feedback loop mechanism. Moreover, MDP stimulation augmented DSC apoptosis. In summary, the results suggest that NOD2 expression in DSCs plays an important role in protecting the embryo and preventing infection in the maternal-fetal interface.

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“…Apoptotic changes have been detected in the maternal–fetal interfaces of the placenta during normal as well as complicated pregnancies [4]. Additionally, apoptosis promotes maternal immune tolerance to the paternal antigens expressed by the trophoblast cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

Placental apoptosis in recurrent miscarriage

Atia

2017

The Kaohsiung J of Med Scie

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Apoptosis is an interactive and dynamic biological process involved in all phases of embryogenesis. We aimed to study the effect of placental apoptosis on recurrent miscarriage (RM). Placental tissue samples were collected from 40 women with RM (study group) and 30 women with sporadic spontaneous abortion (control group). Samples were prepared and stained immunohistochemically with markers for both the apoptotic protein (p53) and anti-apoptotic Bcl-2 antibodies. Our results showed that expression of the apoptotic (p53) protein was significantly increased in the placental tissues of the RM group (p = 0.003). By contrast, the expression of anti-apoptotic (Bcl-2) antibodies was significantly increased in the placental tissues of the control group (p = 0.025). We concluded that placental apoptosis plays a crucial role in pregnancy continuation. However, increased p53 expression in placental tissue in early pregnancy could negatively affect pregnancy continuation.

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Apoptosis and p53 expression in the placental villi of females with unexplained recurrent spontaneous abortion

Cited by 25 publications

References 17 publications

Disordered p53‐MALAT1 pathway is associated with recurrent miscarriage

Disordered p53‐MALAT1 pathway is associated with recurrent miscarriage

Expression and Functional Characterization of NOD2 in Decidual Stromal Cells Isolated during the First Trimester of Pregnancy

Placental apoptosis in recurrent miscarriage

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