Apoptosis as the main type of cell death induced by calcium electroporation in rhabdomyosarcoma cells

Szewczyk, Anna; Saczko, Jolanta; Kulbacka, Julita

doi:10.1016/j.bioelechem.2020.107592

Cited by 15 publications

(4 citation statements)

References 44 publications

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“…The mechanism of death induced by CaEP differs between the results published in the literature. Namely, some studies report apoptosis [45,46], others necrosis [17,44]. It is not that surprising, when considering the role of calcium in various paths of cell death's mechanisms [47].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

et al. 2020

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Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells’ viability with and without calcium administration. For high-voltage pulses, the cell death’s mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer cell mobility was studied using scratch assays. Additionally, we applied calcium-binding fluorescence dye (Fluo-8) to observe the calcium uptake dynamic with the fluorescence microscopy. Moreover, the molecular dynamics simulation visualized the process of calcium ions inflow during μsEP. According to our results calcium electroporation significantly decreases the cells viability by promoting apoptosis. Furthermore, our data shows that the application of pulsed electric fields disassembles the actin cytoskeleton and influences the prostate cancer cells’ mobility.

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Section: Discussionmentioning

confidence: 99%

In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

et al. 2020

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“…Typically, the following events occur sequentially during cellular injury-induced apoptosis: membrane damage, DNA and protein damage, increased reactive oxygen species production, calcium influx into the cells, mitochondrial damage, and ATP depletion [2,6]. The apoptotic pathway occurs sequentially through the intrinsic caspase-dependent pathway, which involves the mitochondrial pathway, leading to the activation of caspases 3, 7, and 9 [47][48][49] and enhancement of cytochrome c and oxidase IV levels [50,51]. In addition, pro-apoptotic factors such as BAX, BAK, and BAD are activated, whereas anti-apoptotic factors such as Bcl-2 and Bcl-xL are inactivated [52][53][54].…”

Section: Discussionmentioning

confidence: 99%

Biphasic Regulation of Apoptosis Following Gastric Irreversible Electroporation Using Tissue Immunohistochemistry of Activated Caspase-3 with TUNEL Method

Jeon,

Chun,

Choi

et al. 2024

Cancers

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The regulation of apoptosis is the primary goal of ablation therapy. Irreversible electroporation (IRE) is a promising non-thermal tissue ablation-based therapy that induces apoptosis by manipulating electrical conditions. This study aimed to investigate IRE-induced gastric tissue apoptosis in response to changes in the electric field intensity, followed by the repair process. Among the 52 rats used in this study, 24 were used to explore apoptosis, and 28 were used to study regeneration. The apoptosis-to-necrosis ratio of the electrical field strength was evaluated using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling and caspase-3 immunohistochemistry. The size of IRE-induced ulcers in the gastric tissue continuously increased with increasing electrical intensity (r2 = 0.830, p < 0.001). The level of apoptosis gradually decreased after peaking at 200 V (1000 V/cm). The size of the 400 V-ablated ulcers continued to decrease, and they were not visible by day 14. The proliferation and migration of epithelial cells with fibroblasts were observed on day 3 and augmented on day 7 post-ablation. This investigation demonstrated the biphasic activation of apoptosis with respect to the electrical field strength. Visually and histologically, IRE-induced gastric ulcers demonstrated complete tissue regeneration after two weeks.

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“…Physical parameters of cells and organelles, such as surface area, height, volume, etc., can be calculated based on RI distribution. HT has been employed in numerous studies to measure cell morphology, including imaging cadmium telluride quantum dots-induced cell apoptosis [ 27 ], analyzing cell morphological changes in response to photodynamic treatment in vitro [ 28 ], investigating sarcoma cell elimination by calcium electroporation [ 29 ], non-invasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine on 4T1 breast tumor cells [ 30 ], and other biological applications [ 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography

Huang,

Lai,

Chen

et al. 2024

Sensors

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This study presents a novel label-free approach for characterizing cell death states, eliminating the need for complex molecular labeling that may yield artificial or ambiguous results due to technical limitations in microscope resolution. The proposed holographic tomography technique offers a label-free avenue for capturing precise three-dimensional (3D) refractive index morphologies of cells and directly analyzing cellular parameters like area, height, volume, and nucleus/cytoplasm ratio within the 3D cellular model. We showcase holographic tomography results illustrating various cell death types and elucidate distinctive refractive index correlations with specific cell morphologies complemented by biochemical assays to verify cell death states. These findings hold promise for advancing in situ single cell state identification and diagnosis applications.

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Apoptosis as the main type of cell death induced by calcium electroporation in rhabdomyosarcoma cells

Cited by 15 publications

References 44 publications

In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

Biphasic Regulation of Apoptosis Following Gastric Irreversible Electroporation Using Tissue Immunohistochemistry of Activated Caspase-3 with TUNEL Method

Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography

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