Apoptosis in human chorionic villi and decidua during normal embryonic development and spontaneous abortion in the first trimester

Kokawa, Katsuji; Shikone, Toshihiko; Nakano, Ryuichi

doi:10.1016/s0143-4004(98)90094-7

Cited by 129 publications

(53 citation statements)

References 24 publications

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“…Also supportive of such a role has been the demonstration in primary human samples of preferential NDRG1 expression in tissues in which apoptosis appears to play a particularly important regulatory role, including periluminal, surface colonic epithelium and placental trophoblast. 25,40,41 We did not observe cytostatic effects on forced expression of recombinant murine NDRG1 in Ba/F3 cells (data not shown). However, evaluation by Northern blot showed that the expression level of recombinant NDRG1 was considerably lower than that of the endogenous gene in response to E2a-Pbx1 (data not shown), making our failure to observe phenotypic changes difficult to interpret.…”

Section: Discussionmentioning

confidence: 78%

The leukemogenic transcription factor E2a-Pbx1 induces expression of the putative N-myc and p53 target gene NDRG1 in Ba/F3 cells

et al. 2001

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The chimeric transcription factor E2a-Pbx1 is expressed as a result of the 1;19 chromosomal translocation in some 5% of cases of pediatric acute lymphoblastic leukemia. We investigated the biological and transcriptional consequences of forced expression of E2a-Pbx1 in the interleukin-3 (IL-3) dependent, bone marrow-derived cell line Ba/F3. We show that forced expression of E2a-Pbx1 induces apoptosis in Ba/F3 cells without apparent effects on cell cycle progression. This pro-apoptotic effect is enhanced on cytokine deprivation. Furthermore, using cDNA representational difference analysis (RDA), we show that these cellular effects are associated with marked induction of the gene NDRG1, which was previously identified as a target of transcriptional repression by N-myc and induction by the tumor suppressor protein p53. We identify a portion of the NDRG1 promoter capable of mediating transcriptional induction by E2a-Pbx1 and show that NDRG1 is also induced on simple IL-3 deprivation of BaF3 cells. Although we show that E2a-Pbx1 induction of NDRG1 is not impaired as a result of targeting p53 using HPV E6, and therefore does not appear to be p53-dependent, our results overall are consistent with the notion that induction of NDRG1 by E2a-Pbx1 may represent part of an apoptotic or cytostatic cellular response to oncogene activation. Leukemia (2001) 15, 362-370.

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Section: Discussionmentioning

confidence: 78%

The leukemogenic transcription factor E2a-Pbx1 induces expression of the putative N-myc and p53 target gene NDRG1 in Ba/F3 cells

et al. 2001

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“…Kokawa et al (1998) salientaram que a apoptose intensa em fase ainda inicial da gestação associa-se com disfunção placentária.…”

Section: Resultsunclassified

“…Se a apoptose ocorre de maneira mais intensa em fase ainda inicial da gestação, pode significar disfunção placentária (Kokawa et al, 1998). Assim, é importante a quantificação morfométrica da apoptose nas células trofoblásticas quando se suspeita de disfunção na maturação placentária.…”

Section: Introductionunclassified

Apoptose no placentomo de cabras gestantes intoxicadas experimentalmente com cipó-preto - Tetrapterys multiglandulosa

Campos

Vasconcelos

Melo

2004

Arq. Bras. Med. Vet. Zootec.

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Apoptose no placentomo de cabras gestantes intoxicadas experimentalmente com cipó-preto -Tetrapterys multiglandulosa RESUMOEstudou-se a ocorrência de apoptose nos placentomos de cabras gestantes intoxicadas experimentalmente com o cipó-preto (Tetrapterys multiglandulosa A.Juss.). Analisou-se morfometricamente a intensidade do processo de apoptose nas células trofoblásticas em cabras controle (grupo III) e em animais submetidos a diferentes dosagens (grupos I e II) de cipó-preto. O grupo I foi composto por quatro cabras gestantes que receberam 10gramas/kg de peso vivo de folhas verdes da referida planta. No grupo II, quatro cabras gestantes receberam 20 gramas/kg de peso vivo de folhas verdes. A quantificação morfométrica da apoptose demonstrou que nas cabras tratadas a apoptose ocorreu com maior intensidade quando comparada com a obtida em animais do grupo-controle. Diferentes dosagens da planta (10 e 20g/kg PV) não foram um fator determinante para a maior ou menor ocorrência de apoptose, apesar de acarretar morte fetal e subseqüente aborto em momentos diferentes. A intensa apoptose em fase ainda inicial da gestação compromete funções normais da placenta, possibilitando uma explicação para a morte fetal e aborto observadas. Conclui-se que a T. multiglandulosa é tóxica para cabras gestantes nas doses de 10 e 20g/kg peso vivo ingeridas durante seis e duas semanas, respectivamente.Palavras-chave: placenta, apoptose, cipó-preto, Tetrapterys multiglandulosa ABSTRACT

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“…1,2,[21][22][23][24] On the other hand, numerous studies have reported that the predominance of Th2 over Th1 cytokines plays some roles in a successful pregnancy. 25,26 Olivares et al previously reported that decidual lymphocytes from human spontaneous abortion cases induced apoptosis in JEG-3 cells, a choriocarcinoma cell line, by interacting with the target cells and IL-2-stimulated decidual lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…1,21,22 In this context, the relationship between granulysin expression and apoptosis in EVTs was explored by immunohistochemistry. Interestingly, the expression of granulysin was detected in the nuclei of EVTs, which were reacted with cytokeratin antibodies, by diaminobenzidine staining (Figure 4A, a).…”

Section: Granulysin Staining Observed In Extravillous Trophoblasts Ofmentioning

confidence: 99%

Granulysin Produced by Uterine Natural Killer Cells Induces Apoptosis of Extravillous Trophoblasts in Spontaneous Abortion

Nakashima

Shiozaki

Myojo

et al. 2008

The American Journal of Pathology

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Apoptosis in human chorionic villi and decidua during normal embryonic development and spontaneous abortion in the first trimester

Cited by 129 publications

References 24 publications

The leukemogenic transcription factor E2a-Pbx1 induces expression of the putative N-myc and p53 target gene NDRG1 in Ba/F3 cells

The leukemogenic transcription factor E2a-Pbx1 induces expression of the putative N-myc and p53 target gene NDRG1 in Ba/F3 cells

Apoptose no placentomo de cabras gestantes intoxicadas experimentalmente com cipó-preto - Tetrapterys multiglandulosa

Granulysin Produced by Uterine Natural Killer Cells Induces Apoptosis of Extravillous Trophoblasts in Spontaneous Abortion

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