Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

Yu, Hyun-Ju; Shin, Ji‐Ae; Yang, In‐Hyoung; Won, Dong‐Hoon; Ahn, Changhyun; Kwon, Hye-Jeong; Lee, Jeong-Sang; Cho, Nam-Pyo; Kim, Eun-Cheol; Yoon, Hae-Sung; Lee, Jae Il; Hong, Seong‐Doo; Cho, Sung‐Dae

doi:10.1016/j.archoralbio.2017.09.024

Cited by 22 publications

(19 citation statements)

References 50 publications

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“…Studies on the ability of CAPE to induce apoptosis in cancer cells have shown that it induces caspase-3/7 activity in AGS and HCT116 after 24 h incubation. These results correlate with those of Yu et al [45], which demonstrated the ability of CAPE activate caspase-3 and induce apoptosis in YD15, HSC-4 and HN22 cells. Similar results were obtained by Kabała-Dzik et al [46] and Dziedzic et al [47]; CAPE successfully induced apoptosis after 24 h incubation in MDA-MB-231 and NHSCC cell lines.…”

Section: Discussionsupporting

confidence: 91%

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

et al. 2020

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The incidence of gastrointestinal cancers is increasing every year. Irinotecan (CPT-11), a drug used in the treatment of colorectal cancer and gastric cancer, is metabolized by carboxylesterases to an active metabolite, SN-38, which is more cytotoxic. CAPE (caffeic acid phenethyl ester) is an active component of propolis, which has a high antibacterial, antiviral, and antineoplastic potential. This study analyses the impact of CAPE on the cytotoxic (MTT assay), genotoxic (comet assay) and proapoptotic (caspase-3/7 activity) potential of irinotecan and its metabolite SN-38 in cultures of gastrointestinal neoplastic cells (HCT116, HT29, AGS). Cytotoxicity and genotoxicity activities of these compounds were carried out in comparison with human peripheral blood lymphocytes (PBLs) in vitro. The antioxidant potential of CAPE was investigated in relation H2O2-induced oxidative stress in the both neoplastic cells and PBLs. CAPE expressed cytotoxic, genotoxic, and pro-apoptotic activity against AGS, HCT116, and HT29 tumor cells. CAPE, in the presence of different concentrations of irinotecan or SN38, decreased the cytotoxicity, genotoxicity, and pro-apoptotic activity in these cell lines, but it has no such action on normal human peripheral blood lymphocytes.

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Section: Discussionsupporting

confidence: 91%

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

et al. 2020

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“…[21][22][23] Other studies have revealed that enhanced expression and activation of the tumor suppressor p53 and activation of pro-apoptotic Bax and caspase-3 may also be induced by CAPE administration. 24,25 These results must also be viewed in context of the limitations that were intrinsic to this type of pilot study. First, and most importantly, this study was limited to only three commercially available oral cancer cell lines.…”

Section: Discussionmentioning

confidence: 96%

Propolis or Caffeic Acid Phenethyl Ester (Cape) Inhibits Growth and Viability in Multiple Oral Cancer Cell Lines.

Saarem

Wang

Farfel

2019

IJMBS

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Objective: Propolis is a natural antimicrobial resin from honeybee hives that contains caffeic acid phenethyl ester (CAPE), which has anti-proliferative activity against some human cancers, including colon, liver, lung and breast – although limited evidence has evaluated this potential in oral cancers. Based upon this information, the primary objective of this study was to evaluate the anti-tumor effects of CAPE against multiple well-characterized oral cancer cell lines. Methods: Using well-characterized oral cancer cell lines (SCC15, SCC25 and CAL27), CAPE was administered at 100 ug/mL to assess any effects on cellular viability or growth over three days. A normal, non-cancerous cell line (HGF-1) was also included. Results: The results of this pilot study demonstrated that CAPE administration significantly reduced both viability and proliferation in all three oral cancer cell lines. Viability was significantly reduced between 30.3% and 35.4% among the oral cancer cell lines (p<0.05), but remained unchanged in the HGF-1 normal cell control (p=0.878). Growth was significantly inhibited between 53.1% and 60.6% among the oral cancer cell lines (p<0.05) but was not affected in the HGF-1 normal cell control (p=0.341). Conclusions: Although the reductions in both cellular viability and proliferation were distinct for each cell line, all exhibited a similar trend and were within a narrowly defined range. These results strongly suggest that CAPE administration had a significant and immediate effect on oral cancer growth and viability and therefore should be considered as the basis for future studies as a potential complementary and alternative therapy for oral cancer. Key words: Caffeic Acid Phenethyl Ester (CAPE), Propolis, Oral cancer, Complementary and alternative medicine.

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“…Puma had the ability to bind to all antiapoptotic proteins of the Bcl-2 family, so its activation enabled programmed death even in cells in which proteins inhibiting apoptosis were active. Moreover, the cytotoxic activity of CAPE was confirmed, and was depended in the time and dose domain [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes

et al. 2020

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Ovarian cancer has the worst prognosis among all gynecological cancers. Therefore, it seems reasonable to seek new drugs that may improve the effectiveness of treatment or mitigate the adverse effects of chemotherapy. Caffeic acid phenethyl ester (CAPE) has many beneficial biological properties. The aim of the study was to assess the anticancer properties of CAPE against serum ovarian carcinoma cells. The morphology of the cells was evaluated in H-E staining and in transmission electron microscopy. The cytotoxic and proapoptotic activity of CAPE was investigated by using the XTT-NR-SRB assay, qRT-PCR analysis of BAX/BCL2 expression, and by cytometric evaluation. CAPE causes constriction in OV7 cells, numerous granulomas were observed in the cytoplasm, the cell nuclei were pyknotic. Autophagosomal vacuoles could suggest the occurrence of aponecrosis. CAPE significantly decreased the lysosomal activity and the total synthesis of cellular proteins. CAPE exhibited, dose and time dependent, cytotoxic activity against OV7 serum ovarian cancer cells. In OV7 cells CAPE induced apoptosis via dysregulation of BAX/BCL2 balance, while activated proapoptotic BAX gene expression level was 10 times higher than BCL2.

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Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

Cited by 22 publications

References 50 publications

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

Antagonistic Effects of CAPE (a Component of Propolis) on the Cytotoxicity and Genotoxicity of Irinotecan and SN38 in Human Gastrointestinal Cancer Cells In Vitro

Propolis or Caffeic Acid Phenethyl Ester (Cape) Inhibits Growth and Viability in Multiple Oral Cancer Cell Lines.

Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes

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