Apoptosis Induced by Dioscin in Hela Cells.

Cai, Jing; Liu, Mingjie; Zhao, Wang; Ju, Yi‐Hsu

doi:10.1248/bpb.25.193

Cited by 103 publications

(53 citation statements)

References 14 publications

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“…8,9) Recent studies have shown that they have cytotoxic properties against several strains of human cancer cells. [10][11][12][13][14] Dioscin [15][16][17] and trillin 18) are the best known diosgenyl saponins with these properties (Fig. 1).…”

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confidence: 99%

The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

Liu

Wang

et al. 2004

Biological & Pharmaceutical Bulletin

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Diosgenyl saponins are the most abundant steroid saponins, and exert a large variety of biological functions. In a previous report, we showed that dioscin was able to induce cytotoxicity and apoptosis in human myeloblast leukemia HL-60 cells. This study further investigated the action mechanisms underlying this effect. The activation of caspase-9 and -3, but not caspase-8, together with the down-regulation of anti-apoptotic Bcl-2 protein, demonstrated that the apoptotic signaling triggered by dioscin was mediated through the intrinsic mitochondriadependent pathway. We also investigated its anti-proliferative effect on human chronic myelogenous leukemia K562 cells. Flow cytometry analysis showed that dioscin treatment induced the accumulation of cells in the G 2 /M phase. Cytomorphology with DAPI and Wright-Giemsa staining demonstrated the enlargement of cell volume and multinucleation in the treated cells. Subsequent apoptosis was delineated with phosphatidylserine externalization and DNA hypodiploidy. Trillin was one of the hydrolysates of dioscin. We demonstrated that it could induce multinucleation in HL-60, K562 and human promyelocytic leukemia NB 4 cells, suggesting its extensive mitotic-arresting effects. As the diosgenyl sapogenin, diosgenin was also shown to be able to induce multinucleation and apoptosis in K562 cells in a similar manner to dioscin. These findings suggest that diosgenyl saponins have the properties to induce mitotic arrest and apoptosis, suggesting that they may be a new kind of antimitotic agent.

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The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

Liu

Wang

et al. 2004

Biological & Pharmaceutical Bulletin

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“…Dioscin (Cai et al, 2002;Zhang et al, 2006;Wang et al, 2007a;Yun et al, 2007;Nguyen et al, 2009), one of the components of RPS, is a preclinical drug showing potent antiproliferative activities against most cell lines from leukemia and solid tumors. In cell culture experiments with HeLa cervix carcinoma cells, it could dose-and time-dependently induce apoptosis via the mitochondrial pathway.…”

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confidence: 99%

Paridis saponins inhibiting carcinoma growth and metastasis In vitro and in vivo

et al. 2011

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Paris polyphylla Smith var. yunnanensis extracts, Rhizoma Paridis saponins (RPS) have been found to show strong antitumor activity. However, few studies have yet investigated pulmonary metastasis treatment with this herb. To detail the effective components in RPS and discuss the preliminary mechanism of antitumor effects in vivo and in vitro, a mixture isolated from RPS was investigated. The main constituents were identified as polyphyllin D, formosanin C, dioscin, Paris H, Paris VII and pennogennin 3-O-α-L-rhamnopyranosyl (1→4)-[β-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside. In our experiments, LA795 cells were exposed to the mixed compounds. Migration inhibition was evaluated by wound healing assay and migration assay in non-cytotoxic dose which was determined by MTT assay. The results demonstrated that the constituent in varying degrees inhibited the migration of the tumor cells in vitro. The mixture also showed antitumor effects on carcinoma in vivo. In conclusion, the mixture is a potent anticancer agent that elicits programmed cell death and inhibits the migration in murine lung adenocarcinoma, both in vitro and in vivo.

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“…Yam exhibits several useful biological properties, such as antioxidative (Hou et al, 2001(Hou et al, , 2002, cholesterol-lowering , and anticholinesterase (Oh et al, 2004) effects, as well as anti-inflammatory (Lee and Lim, 2008), growth hormone-releasing (Lee et al, 2007) and digestionaiding (Jeon et al, 2006) activities. Recent studies revealed that the roots and tuberous roots of Yam contain various bioactive compounds, such as steroidal saponin (Cai et al, 2002), β-sitosterol, antifungal bibenzyls, antifungal phenanthrenes (Coxon et al, 1982;Takasugi et al, 1987), and immune cell-stimulating mucopolysaccharide . Mast cells are one of the most important effector cells in allergic conditions such as asthma, allergic rhinitis, atopic dermatitis, and food allergy (Moon et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells

Jin

Yang

et al. 2011

Arch. Pharm. Res.

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6-Hydroxy-2,7-dimethoxy-1,4-phenanthraquinone (PAQ) isolated from the tuberous roots of Yam (Dioscorea batatas) inhibited cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) dependent prostaglandin D(2) (PGD(2)) generation in mouse bone marrow-derived mast cells in a concentration-dependent manner with IC(50) values of 0.08 μM and 0.27 μM, respectively. In the Western blotting with specific anti-COX-2 antibodies, the decrease of the quantity of PGD(2) was accompanied by a decrease in the COX-2 protein level. But PAQ did not affect COX-1 protein level. In addition, this compound inhibited 5-lipoxygenase (5-LOX) dependent production of leukotriene C(4) in a dose-dependent manner, with an IC(50) of 0.032 μM. These results demonstrate that PAQ has a dual COX-2/5-LOX inhibitory activity. This compound also inhibited the degranulation reaction in a dose-dependent manner with an IC(50) of 2.7 μM. Thus, these results suggest that PAQ may be useful in regulating mast cell-mediated inflammatory diseases.

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Apoptosis Induced by Dioscin in Hela Cells.

Cited by 103 publications

References 14 publications

The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

The Mitotic-Arresting and Apoptosis-Inducing Effects of Diosgenyl Saponins on Human Leukemia Cell Lines

Paridis saponins inhibiting carcinoma growth and metastasis In vitro and in vivo

Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells

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