Apoptosis induced by methanol extract of Potentilla�discolor in human mucoepidermoid carcinoma cells through STAT3/PUMA signaling axis

Abstract

Potentilla discolor has been used in traditional Chinese medicine for the treatment of hyperglycemia. However, the potential role of Potentilla discolor against cancer and its mode of action remain to be fully elucidated. The present study explored the apoptotic effect of methanol extract of Potentilla discolor (MEPD) in human mucoepidermoid carcinoma (MEC) cell lines of salivary glands. MEPD markedly suppressed the growth and induced apoptotic cell death in MC3 and YD15 cells. MEPD treatment significantly upregulated the expression of PUMA and reduced STAT3 phosphorylation. Overexpression of STAT3 partially recovered the growth of MEC cells inhibited by MEPD. In addition, dephosphorylation of STAT3 by cryptotanshinone (a potent STAT3 inhibitor) was sufficient to inhibit the growth of MEC cells and induce apoptosis via affecting PUMA protein. These results suggest that MEPD has a potential anticancer property via the STAT3/PUMA signaling axis in human MEC cells of salivary gland.

Heme Oxygenase-1 is a Key Molecule Underlying Differential Response of TW-37-Induced Apoptosis in Human Mucoepidermoid Carcinoma Cells

“…Several published studies have demonstrated the anti-tumor activities of single or combination chemotherapy with cisplatin, paclitaxel, or cetuximab [1,2]. We recently observed that MECs are effective in chemotherapy through a variety of molecular mechanisms in vitro [3,4]. Despite improvements, current approaches to treating MEC remain disappointing.…”

Potentilla alba Extracts Affect the Viability and Proliferation of Non-Cancerous and Cancerous Colon Human Epithelial Cells

“…The LC-MS profile showed the presence of a large group of polyphenolic compounds (33 compounds) corresponding to the protonated molecular ions of different types of flavonoids, such as quercetin, kaempferol, and isorhamnetin derivatives (12-25, 29, 30) and also caffeoylquinic acids (1-4, 6, 7) or procyanidins (26-28, 32, 33) (Table 1). In addition, flavan-3-ol derivatives (5,(8)(9)(10)(11)31) were confirmed. Individual polyphenolic compounds in PAL1-PAL7 samples were identified by comparison of their UV spectra and the m/z values were identified with those of the selected compounds described in the literature.…”

“…Another study by Paduch and co-workers suggested that analyzed extracts obtained from different species of the genus Potentilla possessed strong antioxidant and immunomodulatory properties reducing the level of IL-10 and increasing the level of IL-6 suggesting that these plants could be used to prevent disturbances in the functioning of the large intestine [30]. Another experiment carried out by Hyun-Ju Yu and co-authors showed that the extract of P. discolor caused a significant increase in the apoptosis of human mucoepidermoid carcinoma cells by increasing the expression of proapoptotic p53 upregulated modulator of apoptosis (PUMA) protein and reducing the phosphorylation signal transducers and activators of transcription [31]. An important finding was also on the P. chinensis species and its impact in osteosarcoma cancer cells.…”

Combined BCL-2 and PI3K/AKT Pathway Inhibition in KMT2A-Rearranged Acute B-Lymphoblastic Leukemia Cells

“…Interestingly, while BBC3 was significantly upregulated in SEM cells, no relevant modulation of TP53 was observed on the gene expression level. This discrepancy could possibly be explained by p53-independent regulation of BBC3 : Recent studies reported that members of the JAK/STAT (STAT3) [ 42 ] and PI3K signaling (AKT, FOXO1, FOXO3A, and GSK3β) [ 43 , 44 , 45 , 46 , 47 , 48 ] pathways as well as Hippo (YAP1) [ 49 ] and MAPK cascade members (ERK, p38, and c-JUN) [ 50 , 51 , 52 ] contributed to the modulation of BBC3 expression. However, none of these molecules exhibited relevant changes in gene expression following PERI incubation.…”