Apoptosis Induction Effect of Curcumin and Its Analogs Pentagamavunon-0 and Pentagamavunon-1 on Cancer Cell Lines

Abstract

Objectives: This experiment aims to investigate the apoptosis effect of curcumin and its analogs pentagamavunon-0 (PGV-0) and PGV-1 on normal and other cancer cell lines.Methods: Growth inhibition effect was investigated using the MTT method. Double staining used acridine orange, 2-(4-aminodiphenyl)-6-indolcarbamidine dihydrochloride and ethidium bromide was performed to determine morphological changes of cells. Detection of PARP, caspase-3, PUMA and BAX using a western blot method was conducted to elucidate the apoptosis effect of the compounds.Results: PGV-1 (2.5 μM) and PGV-0 (5.0 μM) could inhibit T47D-cell growth on 72 h observation, but not for curcumin. DNA staining showed PGV-1 has the strongest apoptosis induction effect on T47D-cells compared to PGV-0 and curcumin as well. Western blot analysis resulted in cleavage PARP (83 kD) on HeLa, T47D, and MCF-7 cells treated with PGV-1 (2.5 µM), PGV-0 (5.0 µM). Curcumin (10.0 µM) just induced apoptosis on T47D-cell and MCF-7 cell, but not HeLa cell. Cleavage PARP resulted by apoptosis process in the cell. PGV-1 (2.5 µM) had a stronger apoptosis effect compared to PGV-0 (5.0 µM) and curcumin (10.0 µM) based on cleaved PARP result qualitatively. On the normal cell (NH3T3), cells that were treated with the compounds resulted in a negative cleavage PARP. This result indicated that the compounds were part of a selectively induced cancer cell line apoptosis process. Conclusion:Curcumin, PGV-0 and PGV-1 could inhibit cell growth by induce apoptosis on cancer cells but not on normal cells, which PGV-1 has strongest apoptosis induction effect on cancer cell lines.

Anti-proliferative and Anti-metastatic Potential of Curcumin Analogue, Pentagamavunon-1 (PGV-1), Toward Highly Metastatic Breast Cancer Cells in Correlation with ROS Generation

“…1 Previously, we reported a new curcumin analogue, namely PGV-1 (Figure 1) to have potential anti-cancer activities against several cancer cells. 2,3 Our studies showed that curcumin and pentagamavunon-1 (PGV-1) inhibit the activity of nuclear factor kappa B (NFκB) in vitro and interact with the HER2 receptor in silico . 4 Furthermore, we also found that PGV-1 increases the cytotoxicity of 5-fluorouracil (5-FU) in WiDR colon cancer cells.…”

The potency of Pentagamavunone‐0 (PGV‐0) as chemopreventive agent for the formation and growth of breast cancer as revealed in 3D model

“…Curcumin has been well known to have anticancer activity (Meiyanto 1999). It is proven to inhibit the growth of cancer cells by inducing apoptosis (Da'i et al 2017) and increasing ROS levels beyond the threshold (Larasati et al 2018). Therefore, PGV0 may inhibit the growth of cancer cells in the same pathway as curcumin.…”

Vanillin Extracted From Proso Millet and Barnyard Millet Induce Apoptosis in Ht-29 and McF-7 Cell Line Through Mitochondria Mediated Pathway

“…1a and b, 2d and e). On various cancer cell lines that might prompt apoptosis through translocation of Bax that activate caspase to induce apoptosis [32].…”