Apoptosis Induction in Hep3B cell line by Lu‐Do‐Huang

Abstract

Pracparatum mungo (Lu‐Do‐Huang) is a derivative of Phaseolus mungo beans of the Leguminosae plant as a traditional medicine for liver detoxifying and anti‐liver cancer effect in southern China. The present study aimed to evaluate Lu‐Do‐Huang ethanol extract (LDHE) on anti‐liver cancer effect with Hep3B cell. The apoptotic activities of LDHE were assessed using flow cytometry, western blotting and immunohistochemical analysis. Our result showed that LDHE significantly inhibited the Hep3B cell growth. LDHE induced Hep3B cells to undergo apoptosis which was determined from Hep3B cell morphology change, increase of apoptotic bodies, apoptotic cells, DNA fragmentations and caspase activity. In conclusion, LDHE lead Hep3B cell to the apoptosis that can be used as precusors in development of anti‐liver cancer drugs. Suppression of apoptosis might contribute to tumor development by mean of accumulation of continuously proliferating cells.