Apoptosis of the hyaloid artery in the rat eye

Taniguchi, Hirokazu; Kitaoka, Takashi; Gong, Huaqing; Amemiya, Tsugio

doi:10.1016/s0940-9602(99)80061-2

Cited by 27 publications

(26 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pericytes can persist at least temporarily in empty basement membrane sleeves (6). However, pericytes on some regressing blood vessels undergo apoptosis (22,67).…”

Section: Discussionmentioning

confidence: 99%

Cellular changes in normal blood capillaries undergoing regression after inhibition of VEGF signaling

Baffert

Le²,

Sennino³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

342

261

View full text Add to dashboard Cite

The vasculature of the embryo requires vascular endothelial growth factor (VEGF) during development, but most adult blood vessels lose VEGF dependence. However, some capillaries in the respiratory tract and selected other organs of adult mice regress after VEGF inhibition. The present study sought to identify the sequence of events and the fate of endothelial cells, pericytes, and vascular basement membrane during capillary regression in mouse tracheas after VEGF signaling was blocked with a VEGF-receptor tyrosine kinase inhibitor AG-013736 or soluble receptor construct (VEGF Trap or soluble adenoviral VEGFR-1). Within 1 day, patency was lost and fibrin accumulated in some tracheal capillaries. Apoptotic endothelial cells marked by activated caspase-3 were present in capillaries without blood flow. VEGF inhibition was accompanied by a 19% decrease in tracheal capillaries over 7 days and 30% over 21 days. During this period, desmin/NG2-immunoreactive pericytes moved away from regressing capillaries onto surviving vessels. Empty sleeves of basement membrane, left behind by regressing endothelial cells, persisted for about 2 wk and served as a scaffold for vascular regrowth after treatment ended. The amount of regrowth was limited by the number of surviving basement membrane sleeves. These findings demonstrate that, after inhibition of VEGF signaling, some normal capillaries regress in a systematic sequence of events initiated by a cessation of blood flow and followed by apoptosis of endothelial cells, migration of pericytes away from regressing vessels, and formation of empty basement membrane sleeves that can facilitate capillary regrowth.

show abstract

“…Pericytes can persist at least temporarily in empty basement membrane sleeves (6). However, pericytes on some regressing blood vessels undergo apoptosis (22,67).…”

Section: Discussionmentioning

confidence: 99%

Cellular changes in normal blood capillaries undergoing regression after inhibition of VEGF signaling

Baffert

Le²,

Sennino³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

342

261

View full text Add to dashboard Cite

show abstract

“…HVS regression involves apoptosis in endothelial cells (31,34) and pericytes (41). This apoptosis is at least partially p53-dependent because HVS regression is slowed or incomplete in inbred p53 Ϫ͞Ϫ BALB͞c (34) and C57BL͞6 (35) mice.…”

Section: Discussionmentioning

confidence: 99%

The Arf tumor suppressor gene promotes hyaloid vascular regression during mouse eye development

Mckeller

Fowler

Cunningham

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

117

140

View full text Add to dashboard Cite

A key tumor suppressor mechanism that is disrupted frequently in human cancer involves the ARF and p53 genes. In mouse fibroblasts, the Arf gene product responds to abnormal mitogenic signals to activate p53 and trigger either cell cycle arrest or apoptosis. Recent evidence indicates that Arf also has p53-independent functions that may contribute to its tumor suppressor activity. Using Arf Ϫ͞Ϫ and p53 Ϫ͞Ϫ mice, we have discovered a p53-independent requirement for Arf in the developmental regression of the hyaloid vascular system (HVS) in the mouse eye. Arf is expressed in the vitreous of the eye and is induced before HVS regression in the first postnatal week. In the absence of Arf, failed HVS regression causes a pathological process that resembles persistent hyperplastic primary vitreous, a developmental human eye disease thought to have a genetic basis. These findings demonstrate an essential and unexpected role for Arf during mouse eye development, provide insights into the potential genetic basis for persistent hyperplastic primary vitreous, and indicate that Arf regulates vascular regression in a p53-independent manner. The latter finding raises the possibility that Arf may function as a tumor suppressor at least in part by regulating tumor angiogenesis.

show abstract

“…Pericyte apoptosis in the course of vessel regression has also been observed during regression of the pupillary membrane and hyaloid vasculature (Taniguchi et al, 1999). In contrast, pericyte numbers did not change during several rounds of tumor vessel regression and regrowth.…”

Section: The Fate Of Pericytes During Vessel Pruning and Regressionmentioning

confidence: 93%

Mechanisms of Vessel Pruning and Regression

2015

View full text Add to dashboard Cite

The field of angiogenesis research has primarily focused on the mechanisms of sprouting angiogenesis. Yet vascular networks formed by vessel sprouting subsequently undergo extensive vascular remodeling to form a functional and mature vasculature. This "trimming" includes distinct processes of vascular pruning, the regression of selected vascular branches. In some situations complete vascular networks may undergo physiological regression. Vessel regression is an understudied yet emerging field of research. This review summarizes the state-of-the-art of vessel pruning and regression with a focus on the cellular processes and the molecular regulators of vessel maintenance and regression.

show abstract

Apoptosis of the hyaloid artery in the rat eye

Cited by 27 publications

References 11 publications

Cellular changes in normal blood capillaries undergoing regression after inhibition of VEGF signaling

Cellular changes in normal blood capillaries undergoing regression after inhibition of VEGF signaling

The Arf tumor suppressor gene promotes hyaloid vascular regression during mouse eye development

Mechanisms of Vessel Pruning and Regression

Contact Info

Product

Resources

About