Apoptotic cell death in human granulosa-lutein cells: a possible indicator of in vitro fertilization outcome

Oosterhuis, G.J.E.; Michgelsen, H.W.B.; Lambalk, Cornelis B.; Schoemaker, J.; Vermes, I.

doi:10.1016/s0015-0282(98)00266-0

Cited by 85 publications

(61 citation statements)

References 7 publications

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“…Granulosa cells are essential in normal follicular maturation process since they produce steroidal hormones and growth factors, and they play a crucial role in follicular atresia [2]. Apoptosis of granulosa cells seems to have a negative effect on conception and pregnancy rates in an in vitro fertilization and embryo transfer (IVF-ET) programme [3,4]. A higher incidence of apoptotic granulosa cells has been associated with a higher incidence of empty follicles, fewer oocytes retrieved and poorer quality of oocytes and embryos [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

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Section: Introductionmentioning

confidence: 99%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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“…Fewer granulosa-lutein cells are apoptotic in women who have an ongoing pregnancy after IVF treatment than in women who do not conceive [23,24]. Women over the age of 38 have Fig.…”

Section: Discussionmentioning

confidence: 99%

“…2 There was no correlation between total dose of recFSH and mitochondrial gene expression ratio Fig. 3 Mitochondrial gene expression ratio in patients who received hMG was statistically higher than that of patients under recFSH granulosa cells that contain substantially lower levels of normal mitochondria as compared with women 34 years or younger [23,24]. Moreover, Fatum et al [25] hypothesized that during the process of reproductive aging, mitochondrial membrane potential may deteriorate as a result of free-radical accumulation.…”

Section: Discussionmentioning

confidence: 99%

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Saito

Yamashita

Higuchi

et al. 2013

Reprod Medicine & Biology

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Purpose Examination of the mitochondrial mRNA expression in granulosa cells from an unspecified population of infertile patients to evaluate whether recombinant follicle stimulating hormone (recFSH) is more effective in producing higher quality embryo rates compared with human menopausal gonadotropin (hMG). Method Thirty-nine patients who underwent the in vitro fertilization and embryo transfer program were retrospectively examined. Patients were administered recFSH (n = 18) or hMG (n = 20) in a long protocol where GnRH agonist was used. Granulosa cells were obtained during oocyte retrieval and examined for mitochondria mRNA expression ratio against GAPDH. Expressions of mitochondria mRNA were evaluated by real-time PCR analysis. Results The high-quality embryo rate in the hMG cycle was higher than in the recFSH cycle, and the total dose of hMG showed a positive correlation with the expression level of mitochondrial genes in granulosa cells. Moreover, mitochondria mRNA expression was higher in the hMG cycle than in the recFSH cycle. Conclusions Compared with recFSH, hMG induces a higher mitochondrial gene expression ratio in granulosa cells at the time of oocyte retrieval and, therefore, may lead to higher quality embryo rates.

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“…Previous studies have investigated the incidence of GC apoptosis and its relation to IVF/ICSI results [8][9][10][11][12][13]. However, it is important to recognize that the majority of these studies assessed apoptosis of cells suspended in follicular aspirates.…”

Section: Discussionmentioning

confidence: 99%

“…GC apoptosis and/or necrosis may influence oocyte quality and future embryo development. Studies have reported a positive correlation of GC number with poor IVF/ICSI results [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

Filho¹,

Ribeiro

Rocha

et al. 2008

J Assist Reprod Genet

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Objective To determine whether gonadotropin releasing hormone (GnRH)-agonist or -antagonist induces higher percentages of cumulus cell apoptosis and if the use of either is detrimental to ART outcomes. Patients Women in a private facility under treatment for IVF had their cumulus cells isolated and analyzed by flow cytometry. Viable, apoptotic, and dead cumulus cell rates related to ovarian stimulation by GnRH-agonist orantagonist were measured and compared with fertilization and implantation rates. Results Treatment with GnRH-agonist produced a greater number of follicles than treatment with GnRH-antagonist. No differences in implantation and pregnancy rates were found. While cumulus cell (CC) apoptosis was positively correlated with estradiol on the day of hCG administration, no significant difference in the percentage of apoptotic cells between treatments was detectable. Additionally, implantation rate and the average follicular estradiol production on the day of hCG administration were no different between treatments.Conclusions GnRH-agonist or -antagonist treatment protocols induce similar levels of apoptosis in CCs and are not detrimental to ART outcomes.

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Apoptotic cell death in human granulosa-lutein cells: a possible indicator of in vitro fertilization outcome

Cited by 85 publications

References 7 publications

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

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