Apoptotic Index from Fine Needle Aspiration Cytology as a Criterion To Predict Histologic Grade of Non-Hodgkin’s Lymphoma

Symmans, W. Fraser; Cangiarella, Joan; Symmans, Pennie; Cohen, J; Yee, Herman; Bennett, Gregory J.; Amorosi, Edward L.; Waisman, Jerry

doi:10.1159/000326360

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…Similarly to Philips et al (2000), the values of AI obtained in our study were not statistically significant among lymphoma grades. However, data from human medicine indicates that such differences exist between low and high NHLs irrespective of the method of AI assessment: counting of apoptotic bodies (Symmans et al 2000) or TUNEL positive cells (Zuo et al 2006(Zuo et al , 2007. A possible explanation for this could be the small number of indolent lymphoma cases or the specificity of the TUNEL assay.…”

Section: Discussionmentioning

confidence: 99%

Survivin expression in correlation with apoptotic activity in canine lymphomas

Sokołowska¹,

Urbańska²

2017

Polish Journal of Veterinary Sciences

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Survivin regulates cell cycle and mitosis and has antiapoptotic properties. Because of its dual function survivin has been the subject of much research focusing on its role in tumorigenesis and the relationship between survivin expression and apoptotic and/or proliferative activity in many types of human tumor including non-Hodgkin's Lymphomas. Such studies have not been conducted in canine lymphomas. The aim of this study was to evaluate the expression of survivin in canine lymphomas of low (5/25) and high (20/25) grades in relation to apoptotic markers (apoptotic index and index of caspase-3). Survivin was found in all examined lymphomas. Most tumors (18/25) showed survivin expression in 10%-25% of positive cells. Only in single cases was lower (0-10% positive cells, 1/25) or higher (25%-50% and >50% positive cells, 5/25 and 1/25, respectively) survivin expression. No significant differences between mean values of either index of survivin or apoptotic index was found between low and high grade lymphomas. However, such a difference among lymphoma grades was shown regarding the caspase-3 index. No correlation between the survivin index and either the apoptotic index or caspase-3 index was found, irrespective of the method of quantification: in whole specimens or in areas of low and high survivin expression. Positive correlation was consistently noted only between both apoptotic markers. The results indicate that survivin is commonly expressed in canine lymphomas. It seems that survivin does not exhibit anti-apoptotic activity in canine lymphomas. Lack of correlation between survivin expression and apoptotic markers could indicate its potential role in cell cycle activation in lymphoma cells.

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Section: Discussionmentioning

confidence: 99%

Survivin expression in correlation with apoptotic activity in canine lymphomas

Sokołowska¹,

Urbańska²

2017

Polish Journal of Veterinary Sciences

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“…However, even though its ability to survive may be improved as a result of oncogenic evolution, an emerging pre-malignant or malignant cell population does not lose its capacity to undergo apoptosis. Indeed, apoptosis indices indicate an association between high-grade malignancies and high-rate apoptosis and therefore prominent apoptosis is suggestive of an adverse prognosis [122][123][124][125][126]. High-grade malignancies have high proliferation rates.…”

Section: Relationships Between Apoptosis and Oncogenesismentioning

confidence: 99%

Microenvironmental influences of apoptosis in vivo and in vitro

Gregory

Pound

2010

Apoptosis

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The apoptosis program of physiological cell death elicits a range of non-phlogistic homeostatic mechanisms-"recognition, response and removal"-that regulate the microenvironments of normal and diseased tissues via multiple modalities operating over short and long distances. The molecular mechanisms mediate intercellular signaling through direct contact with neighboring cells, release of soluble factors and production of membrane-delimited fragments (apoptotic bodies, blebs and microparticles) that allow for interaction with host cells over long distances. These processes effect the selective recruitment of mononuclear phagocytes and the specific activation of both phagocytic and non-phagocytic cells. While much evidence is available concerning the mechanisms underlying the recognition and responses of phagocytes that culminate in the engulfment and removal of apoptotic cell bodies, relatively little is yet known about the non-phagocytic cellular responses to the apoptosis program. These responses regulate inflammatory and immune cell activation as well as cell fate decisions of proliferation, differentiation and death. Here, we review current knowledge of these processes, considering especially how apoptotic cells condition the microenvironments of normal and malignant tissues. We also discuss how apoptotic cells that persist in the absence of phagocytic clearance exert inhibitory effects over their viable neighbors, paying particular attention to the specific case of cell cultures and highlighting how new cell-corpse-clearance devices-Dead-Cert Nanoparticles-can significantly improve the efficacy of cell cultures through effective removal of non-viable cells in the absence of phagocytes in vitro.

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Apoptotic Cell Clearance by Macrophages: Relevance to Tumor Pathogenesis

Debatin¹,

Fulda²

2006

Apoptosis and Cancer Therapy

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Apoptotic Index from Fine Needle Aspiration Cytology as a Criterion To Predict Histologic Grade of Non-Hodgkin’s Lymphoma

Cited by 5 publications

References 27 publications

Survivin expression in correlation with apoptotic activity in canine lymphomas

Survivin expression in correlation with apoptotic activity in canine lymphomas

Microenvironmental influences of apoptosis in vivo and in vitro

Apoptotic Cell Clearance by Macrophages: Relevance to Tumor Pathogenesis

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