Appia: Simpler chromatography analysis and visualization

Abstract

Chromatography is an essential family of assays for molecular biology and chemistry. Typically, only a qualitative assessment of peak height, position, and shape are sufficient to proceed. Additionally, chromatography instrument software is proprietary and often locked to a single computer, making data analysis and sharing difficult. Since each manufacturer reports the data in their own proprietary format, performing analysis of experiments which use multiple instruments or sharing data between labs is also challenging. Here we present Appia, a free, open-source chromatography processing and visualization package focused on making analysis, collaboration, and publication quick and easy.

The utilization of open‐source microcontroller boards and single‐board computers in liquid chromatography

“…To build upon standard data logging functionality, a Raspberry Pi-based hardware/software interface combined DAQ functionality with signal filtering and the calculation of chromatographic figures of merit for an LC separation, with results of the calculations comparable to instrument manufacturer software [119]. The processing of file types generated by commercial software packages has also been achieved using the open-source Appia program that can be operated on a Raspberry Pi [120]. In a study comparing broadening effects from different LC detectors, a Pi was used as a data logger for the FLD that was investigated [121].…”

Structural Insights into Subunit-Dependent Functional Regulation in Epithelial Sodium Channels

“…Data was acquired with the Axon Instruments Molecular Devices Axoclamp 200B and Axon DigiData 1550B. Data was analyzed with pClamp, GraphPad Prism, and ABF Plotter 75.…”