Applicability of cord blood‐derived unrestricted somatic stem cells in tissue engineering concepts

Degistirici, Özer; Jäger, Markus; Knipper, A.

doi:10.1111/j.1365-2184.2008.00536.x

Cited by 17 publications

(18 citation statements)

References 51 publications

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“…Furthermore, their use does not raise ethical concerns [2,33,34] . The differentiation potential of USSCs into the osteogenic lineage is well known and has been verified by many research groups [36,40,41] . They can be differentiated into the osteogenic lineage both spontaneously and when cultured with specific supplements [36] .…”

Section: Discussionmentioning

confidence: 80%

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Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

et al. 2011

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Aim: Bioartificial bone tissue engineering is an increasingly popular technique to solve bone defect challenges. This study aimed to investigate the interactions between matrix composition and appropriate cell type, focusing on hydroxyapatite (HA), to achieve a more effective combination for bone regeneration. Methods: Human unrestricted somatic stem cells (USSCs) were isolated from placental cord blood. The cellular and molecular events during the osteo-induction of USSCs were evaluated for 21 d under the following conditions: (1) in basal culture, (2) supplemented with hydroxyapatite nanoparticle (nHA) suspension, and (3) seeded on electrospun aligned nanofibrous poly-ε-caprolactone/poly-L-lactic acid/nHA (PCL/PLLA/nHA) scaffolds. The scaffolds were characterized using scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR) and tensile test. Results: Maintenance of USSCs for 21 d in basal or osteogenic culture resulted in significant increase in osteoblast differentiation. With nHA suspension, even soluble osteo-inductive additives were ineffective, probably due to induced apoptosis of the cells. In contrast to the hindrance of proliferation by nHA suspension, the scaffolds improved cell growth. The scaffolds mimic the nanostructure of natural bone matrix with the combination of PLLA/PCL (organic phase) and HA (inorganic phase) offering a favorable surface topography, which was demonstrated to possess suitable properties for supporting USSCs. Quantitative measurement of osteogenic markers, enzymatic activity and mineralization indicated that the scaffolds did not disturb, but enhanced the osteogenic potential of USSCs. Moreover, the alignment of the fibers led to cell orientation during cell growth. Conclusion:The results demonstrated the synergism of PCL/PLLA/nHA nanofibrous scaffolds and USSCs in the augmentation of osteogenic differentiation. Thus, nHA grafted into PCL/PLLA scaffolds can be a suitable choice for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 80%

“…In an experiment by Babister et al [1] , both the activity and the transcription of ALPase in hMSCs were highest on d 21. Conversely, Degistirici et al [41] found that USSCs expressed the most active ALPase on d 7 during osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 95%

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

et al. 2011

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“…As USSCs also show a high production of VEGF and FGF2 and express VEGF Receptor-2 the transplantation into a bone or tissue defect might support the repair process not only by initiating mineralization but also by creating an angiogenic environment. 64 …”

Section: Characterization Of Non-adherent Cells From Fresh and Cryoprmentioning

confidence: 99%

Future of cord blood for non-oncology uses

Kögler

Critser

Trapp

et al. 2009

Bone Marrow Transplant

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“…In addition, other investigators [57][58][59][60][61] and our group [61][62][63][64][65][66] showed that USSC can be stimulated to differentiate into cells of the three germ layer lineages, endodermal, ectodermal and mesodermal [62]. USSCs have an initial population doubling time of about 39 h, which decreased only slightly with increasing passage number, but cells maintained their proliferation abilities up to passage 23.…”

Section: Embryonic Sc (Esc) Trans-differentiationmentioning

confidence: 89%

“…[46] who recommend 10% FCS for CB-MSC in vitro-expansion, own experiences have shown advantages for higher FCS concentations supplemented by 10 −5 -10 −7 M dexamethasone [62]. Following this cultivation protocol colony forming units will appear after 5-10 days after initial cultivation.…”

Section: Embryonic Sc (Esc) Trans-differentiationmentioning

confidence: 99%

Cord Blood—An Alternative Source for Bone Regeneration

Jäger

Zilkens

Bittersohl

et al. 2009

Stem Cell Rev and Rep

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Bone regeneration is one of the best investigated pathways in mesenchymal stromal cell (MSC) biology. Therefore strong efforts have been made to introduce tissue engineering and cell therapeutics as an alternative treatment option for patients with bone defects. This review of the literature gives an overview of MSC biology aiming for clinical application including advantages but also specific challenges and problems which are associated with cord blood derived stromal cell (CB-MSC) as a source for bone regeneration. The use of postnatal CB-MSC is ethically uncomplicated and requires no invasive harvesting procedure. Moreover, most data document a high osteogenic potential of CB-MCS and also low immunoreactivity compared with other MSC types. The expression profile of CB-MSC during osteogenic differentiation shows similarities to that of other MSC types. Within the umbilical cord different MSC types have been characterized which are potent to differentiate into osteoblasts. In contrast to a large number of in vitro investigations there are only few in vivo studies available so far.

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Applicability of cord blood‐derived unrestricted somatic stem cells in tissue engineering concepts

Abstract: USSCs when transplanted into a bone defect might support the repair process not only by pure remineralization but also by installation of angiogenic environment.

Cited by 17 publications

References 51 publications

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

Future of cord blood for non-oncology uses

Cord Blood—An Alternative Source for Bone Regeneration

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