Applicability of Preoperative Nuclear Morphometry to Evaluating Risk for Cervical Lymph Node Metastasis in Oral Squamous Cell Carcinoma

Karino, Masaaki; Nakatani, Eiji; Hideshima, Katsumi; Nariai, Yoshiki; Tsunematsu, Kohji; Ohira, Koichiro; Kanno, Takahiro; Asahina, Izumi; Kagimura, Tatsuo; Sekine, Joji

doi:10.1371/journal.pone.0116452

Cited by 11 publications

(14 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neck node metastasis is an important prognostic factor in oral SCC. However, primary tumor site was not found to be associated with cervical lymph node metastasis under multivariable regression analysis on 88 patients only collected retrospectively from 1981 to 2012 [5]. Under another study (Kailiu Wu et al (2015)) histological grade and neurovascular invasion were associated with the risk of cervical lymph node metastasis of early tongue SCC (n = 171) [13].…”

Section: Discussionmentioning

confidence: 91%

“…Metastasis to cervical lymph nodes may be because of filtering effect of the rich lymphatic system. Nodal metastasis strongly influences the five year survival rate, prognosis of oral cancer [5] and significantly affects regional control and survival [6] [7] [8] [9] [10]. Also, Metastasis in nodes is a complex multi-step process and cannot be explained by enlargementonly [11] [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Epidemiological Model to Find out Factors Associated with Nodal Involvement among Indian Oral Cancer Patients

Singh¹,

Deo²,

Dwivedi³

et al. 2018

OJEpi

View full text Add to dashboard Cite

In India, the most common cancer among men is cancer of lip-oral and its incidence is further increasing. Nodal metastasis is an important prognostic factor in oral cancer. Previous studies on factors associated with nodal involvement are mainly focused on clinical exploration, and there is very little work in statistical modeling for nodal involvement. Also, the available studies have limited covariates and their varying forms. Further, studies available from India have mainly focused either on occult nodal metastasis only or a specific site of oral or stages. Hence, in order to identify epidemiological determinants of nodal metastasis, objective of this study was to develop a regression model to find out factors associated with nodal involvement and assess its validity. 945 histopathologically proven oral squamous cell carcinoma (OSCC) patients who went under surgery including neck dissection during 1995-2013 at the Department of Surgical Oncology, Dr. BRA-IRCH, AIIMS, New Delhi, India, were included for model building. Another data of 204 patients available during 2014-2015 was used for the temporal validation of the developed model. To assess the factors associated with nodal involvement, stepwise multivariable logistic regression procedure was used and results are presented as odds ratio and corresponding 95% confidence interval (CI). Pain at the time of presentation [1.34 (1.02 to 1.77)], sub mucous fibrosis (SMF) [0.45 (0.21 to 0.95)], palpable neck node [2.38 (1.69 to 3.35)], tongue [1.63 (1.07 to 2.46)] as compared to buccal mucosa and degree of differentiation [1.41 (1.05 to 1.89)] were found to be significantly associated with nodal involvement. Further, diagnostic performance of the developed model was found to be satisfactory on temporal validation. These data suggest that, Pain at time of presentation, presence of clinical neck node, SMF, degree of differentiation and oral site are the most probable factors associated with nodal How to cite this paper: Singh

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

An Epidemiological Model to Find out Factors Associated with Nodal Involvement among Indian Oral Cancer Patients

Singh¹,

Deo²,

Dwivedi³

et al. 2018

OJEpi

View full text Add to dashboard Cite

show abstract

“…Nuclear polymorphisms have been associated with poor prognosis in head and neck cancer . Manual count of nuclei in this study identified >7 large and >6 atypical nuclei per image to detect dysplastic lesions.…”

Section: Discussionmentioning

confidence: 82%

“…Nuclear polymorphisms have been associated with poor prognosis in head and neck cancer. 21,22 Manual count of nuclei in this study identified >7 large and >6 atypical nuclei per image to detect dysplastic lesions. In analogy, it was shown that only ≥3 very large, anaplastic nuclei and ≥3 multinucleated cells are correlated to worse survival in p16+ oropharyngeal carcinoma.…”

Section: = 143 Images) = 94 Images) Automentioning

confidence: 86%

Advances toward fully automated in vivo assessment of oral epithelial dysplasia by nuclear endomicroscopy—A pilot study

Liese

Winter

Glass

et al. 2017

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…Our result was in accordance to studies conducted by various authors. 8,[20][21][22][23][24][25] The metastasis to lymph nodes manifests when specific cellular and nuclear changes occur in tumor cells, which represent a distinctive clone. The tumor cells undergo certain changes phenotypically that aids in transportation of these cells to the lymphatic stream.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Nuclear Morphometry in Oral Squamous Cell Carcinoma: A Retrospective Study

Swain¹,

Patel²,

Pathak³

et al. 2017

Journal of Contemporary Dentistry

View full text Add to dashboard Cite

Aim: To evaluate and compare various nuclear morphometric parameters in different histopathological grades of oral squamous cell carcinoma (OSCC) by using computerized image analysis and also to correlate it with regional lymph node metastasis. Materials and methods:This retrospective study was conducted on paraffin blocks of 40 tissue specimens of OSCC cases treated with neck dissection, which were retrieved from the archives of the Department of Oral Pathology and Microbiology, Mahatma Gandhi Mission's Dental College and Hospital, Navi Mumbai, India. All cases were histopathologically graded as well, moderately, and poorly differentiated OSCC. Further, they were also categorized based on pathological lymph node status as with or without lymph node metastasis. Sections from tumor proper were subjected to Feulgen nuclear staining technique. Images of 10 microscopic fields at the deepest invading part of tumor were captured randomly and 100 nuclei of tumor cells with clear, complete, nonoverlapping outlines were selected in each case. Nuclear morphometric parameters, such as large diameter, small diameter, nuclear area, and nuclear perimeter were measured for each of the 100 cells.Results: An increase in mean nuclear area coefficient of variation (NACV) was observed in OSCC cases with lymph node metastasis pN(+) than in those without lymph node metastasis pN(-), (p = 0.67). A significant increase in nuclear area and perimeter was observed in pN(+) cases (p < 0.01). A significant decrease in circular rate and increase in largest to smallest nuclear diameter (L/S) ratio (p < 0.01) was observed in pN(+) cases. On comparing the nuclear morphometric parameters with different histopathological grades of OSCC, we found an increase in mean NACV values from well-differentiated OSCC to moderately differentiated and poorly differentiated OSCC significant difference (LSD) post hoc test, indicated a difference only between well-differentiated and poorly differentiated cases of OSCC (p = 0.132). Conclusion:Our observations reveal that tumor cells with greater nuclear dimension and more elliptical shape tend to show increased incidence of nodal metastasis. Also, a positive inclination was observed in nuclear size and shape with increasing histopathological grades of OSCC. However, our data warrant a large-scale study to establish nuclear morphometry as a quantitative objective parameter and also for the rational application of the same.

show abstract

Applicability of Preoperative Nuclear Morphometry to Evaluating Risk for Cervical Lymph Node Metastasis in Oral Squamous Cell Carcinoma

Cited by 11 publications

References 40 publications

An Epidemiological Model to Find out Factors Associated with Nodal Involvement among Indian Oral Cancer Patients

An Epidemiological Model to Find out Factors Associated with Nodal Involvement among Indian Oral Cancer Patients

Advances toward fully automated in vivo assessment of oral epithelial dysplasia by nuclear endomicroscopy—A pilot study

Evaluation of Nuclear Morphometry in Oral Squamous Cell Carcinoma: A Retrospective Study

Contact Info

Product

Resources

About