Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium Brasiliense L.

Abstract

Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Agency, ANVISA) has canceled the registration of these products, and their use was discontinued because of a lack of studies to characterize the plant raw material and ensure the effectiveness and safety of its use. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from L. brasiliense roots, by the UV/Vis spectrophotometric method. L. brasiliense roots were extracted in acetone:water (7:3, v/v-10% w/v). The crude extract was used to develop a method for TP assay. The method was validated according to national and international guidelines. The optimum conditions for analysis time, wavelength, and standard substance were 30 min, 760 nm, and pyrogallol, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.

Influence of hot water blanching process on phenolic profile and antioxidant activity of Cinnamomum porrectum herbal tea

“…The results suggested that pyrogallol was the best reference substance to exhibit H + donor, electron transfer and or both abilities, due to the highest number of hydroxyl groups being proportional to its small molar mass. In fact, pyrogallol has only one ring and no other substituted groups so that make less influenced by electronic interactions such as steric or resonance effects [36]. Therefore, pyrogallol not only showed highest of TPC but also showed highest antioxidant activities when determined as ABTS and FRAP assay, but not DPPH assay which gallic acid expressed higher value compared with pyrogallol this result confirmed that main bioactive compounds responded to high polar compounds due to aqueous extraction method.…”

Effects of Salt and Water Stress on Plant Growth and on Accumulation of Osmolytes and Antioxidant Compounds in Cherry Tomato

“…Total phenolic compounds were quantified by reaction with the Folin-Ciocalteu reagent, according to Blainski et al (2013). Absorbance was measured at 765 nm and the results expressed in equivalents of gallic acid (mg eq.…”

DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIA ANALÍTICA POR ESPECTROFOTOMETRIA NO VISÍVEL PARA QUANTIFICAÇÃO DE TANINOS TOTAIS NA CASCA DO CAULE DE Simarouba amara Aubl.

“…O método foi considerado específico quando os coeficientes angulares das equações da reta de linearidade e especificidade foram considerados semelhantes (BLAINSKI et al, 2013).…”

“…Os testes foram realizados em triplicata para cada nível de concentração, com ensaios realizados em triplicata, sendo calculado o percentual de recuperação. O método foi considerado exato quando o percentual de recuperação atingiu a faixa entre 85 e 115% (BRASIL, 2003;BLAINSKI et al, 2013).…”

“…A mudança nas concentrações de carbonato de sódio levou em consideração dados da literatura: 7,5% (BLAINSKI et al, 2013), 10,75% (GLASL, 1983 para determinação do teor de taninos totais quando comparado com os demais. Além disso, aumento na sua quantidade resultou em incremento da resposta ao método.…”