Application of a behavioural and biochemical endpoint in ecotoxicity testing with Exaiptasia pallida.

Ianna, Madeline Louise; Reichelt‐Brushett, Amanda; Howe, Pelli Louise; Brushett, Donald J.

doi:10.1016/j.chemosphere.2020.127240

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…The absence of a calcium carbonate skeleton is a major feature of scleractinians, and the availability of symbiotic and aposymbiotic sea anemones has been noted (Howe et al ., 2012). Sea anemones can be easily maintained in laboratory settings and in large cultures, and research has shown that they are susceptible to a variety of pollutants (Anjos et al ., 2017; Howe et al ., 2012, 2017; Trenfield et al ., 2017; Ianna et al ., 2020; Vitale et al ., 2020; Rosner et al ., 2023). The sea anemones Aiptasia pulchella ( Exaiptasia pallida ), Anemonia viridis and A. equina (Howe et al ., 2012; Trenfield et al ., 2017; Vitale et al ., 2020) have been suggested as the best model taxa for research.…”

Section: Non‐standard Modelsmentioning

confidence: 99%

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

Rosner,

Ballarin,

Barnay‐Verdier

et al. 2023

Biological Reviews

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Aquatic invertebrates play a pivotal role in (eco)toxicological assessments because they offer ethical, cost‐effective and repeatable testing options. Additionally, their significance in the food chain and their ability to represent diverse aquatic ecosystems make them valuable subjects for (eco)toxicological studies. To ensure consistency and comparability across studies, international (eco)toxicology guidelines have been used to establish standardised methods and protocols for data collection, analysis and interpretation. However, the current standardised protocols primarily focus on a limited number of aquatic invertebrate species, mainly from Arthropoda, Mollusca and Annelida. These protocols are suitable for basic toxicity screening, effectively assessing the immediate and severe effects of toxic substances on organisms. For more comprehensive and ecologically relevant assessments, particularly those addressing long‐term effects and ecosystem‐wide impacts, we recommended the use of a broader diversity of species, since the present choice of taxa exacerbates the limited scope of basic ecotoxicological studies.This review provides a comprehensive overview of (eco)toxicological studies, focusing on major aquatic invertebrate taxa and how they are used to assess the impact of chemicals in diverse aquatic environments. The present work supports the use of a broad‐taxa approach in basic environmental assessments, as it better represents the natural populations inhabiting various ecosystems. Advances in omics and other biochemical and computational techniques make the broad‐taxa approach more feasible, enabling mechanistic studies on non‐model organisms. By combining these approaches with in vitro techniques together with the broad‐taxa approach, researchers can gain insights into less‐explored impacts of pollution, such as changes in population diversity, the development of tolerance and transgenerational inheritance of pollution responses, the impact on organism phenotypic plasticity, biological invasion outcomes, social behaviour changes, metabolome changes, regeneration phenomena, disease susceptibility and tissue pathologies. This review also emphasises the need for harmonised data‐reporting standards and minimum annotation checklists to ensure that research results are findable, accessible, interoperable and reusable (FAIR), maximising the use and reusability of data. The ultimate goal is to encourage integrated and holistic problem‐focused collaboration between diverse scientific disciplines, international standardisation organisations and decision‐making bodies, with a focus on transdisciplinary knowledge co‐production for the One‐Health approach.

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Section: Non‐standard Modelsmentioning

confidence: 99%

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

Rosner,

Ballarin,

Barnay‐Verdier

et al. 2023

Biological Reviews

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“…For the toxicity test, the microcrustacean Artemia sp. test organisms used in the ABNT NBR 16530:2016 standard and in several studies (Diaz-Sosa et al, 2020;Da Fontoura et al, 2020;Cavion et al, 2020;Ianna et al, 2020). The experiment was repeated with the MCA working with a volume of 50 mL of synthetic effluent.…”

Section: Test Of Toxicitymentioning

confidence: 99%

Design of a hospital laundry effluent clarification system

Sobral

Novaes

Lins

et al. 2022

RSD

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Approximately 20% of the mineral coal ash (MCA) generated worldwide, during the combustion of this fossil fuel, has been used to produce Portland cement. Other applications include soil improvement, ceramic industry, catalysis, and an application with special interest for this work which is the synthesis of zeolites. A hospital effluent clarification system was developed using MCA as an adsorbent for substances responsible for the color of the effluent generated by the laundry. A perfect mix tank promotes intimate contact between MCA and the effluent. At the exit of the mixing tank, a pH sensor correlated the value of this variable with the concentration of MCA in the mixture inside the tank. The information about the pH of the mixture was then sent to a programmable logical controller (PLC), which regulated the motor speed of a screw conveyor, with the aid of a frequency inverter. A color removal efficiency of the order of 99.8% was achieved. The proposed clarification system can operate both in batch and continuous. In the first case, the ideal operating conditions can be set. The strategy for adjusting operating conditions is to obtain an adequate nominal capacity for the system by reducing or increasing the number of cyclones per battery or the number of cyclone batteries.

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“…Yet, behavioral ecotoxicology has received less attention due to a lack of user-friendly tools for quantitative data acquisition (Faimali et al, 2017). As a result, little is known about behavioral stress responses in cnidarians (Ianna et al, 2020), and most studies to date have been based on tedious manual analysis of observational data such as manually tracing swimming pathways and image analysis (Reichelt-Brushett and Harrison, 2004;Kwok and Ang, 2013;Antonio-Martıńez et al, 2020;Ianna et al, 2020). Some more advanced approaches have been applied to tracking coral larvae movement including the reconstruction from film using Matlab Image Processing toolbox (Martıńez-Quintana et al (2015)).…”

Section: Introductionmentioning

confidence: 99%

Applying behavioral studies to the ecotoxicology of corals: A case study on Acropora millepora

et al. 2022

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Behavioral responses are considered sensitive and effective indicators of organism stress. As the demand for standardized coral toxicity tests grows, innovative tools that allow for automatic and quantitative measurements of these behaviors may complement ecotoxicological studies. The rapid growth of competitive marine algae in aquaculture systems is a major issue for generating coral spat for reef restoration, and the application of non-toxic antifouling (AF) coatings might effectively mitigate this issue. While these coatings do not appear to be toxic to sensitive coral larvae, their potential to affect larval mobility has not been tested. In this context, we tested the effect of three recently-developed and potentially non-toxic AF coatings: (i) antiadhesive, (ii) cerium dioxide (CeO2-x) nanoparticle, and (iii) encapsulated biocide dichlorooctylisothiazolinone (DCOIT) on the swimming velocity and activity of Acropora millepora coral larvae for potential use in reef-restoration activities. The behavior of 32 coral larvae per AF treatment were recorded, each for 25 min, in a self-constructed dark box with two camera recording sets in parallel. The tracking analysis was performed with the software Noldus EthoVision XT. The mean larval swimming velocity on control tiles of 93.1 ± 5.6 mm min-1 (and activity of 62.8 ± 5.2%) was nearly 2-fold faster (higher) than on the antiadhesive, (CeO2-x) nanoparticle and DCOIT coatings, respectively. Larvae exposed to the DCOIT-coated tiles remained almost stationary. Although the underlying cause and consequence of these results require further investigation, tracking of coral larval swimming behavior was identified as a reliable and feasible method for assessing potential non-lethal responses to AF coatings. As changes in behavior could have significant consequences for larval survival and settlement, they are important endpoints to consider, and the quantification of behavioral responses may be a meaningful and sensitive tool. Therefore, we recommend the use of behavioral studies for coral larval assessments in ecotoxicology as a valuable endpoint. For methodological standardization and implementation, our study also features a detailed guide for video-processing and track analysis of A. millepora coral larvae in EthoVision.

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Application of a behavioural and biochemical endpoint in ecotoxicity testing with Exaiptasia pallida.

Cited by 13 publications

References 32 publications

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

A broad‐taxa approach as an important concept in ecotoxicological studies and pollution monitoring

Design of a hospital laundry effluent clarification system

Applying behavioral studies to the ecotoxicology of corals: A case study on Acropora millepora

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