Application of a capillary‐assembled microfluidic system for separation of cephalosporins

Abstract

This paper demonstrates a simple and easy setting up of a fused-silica capillary-assembled microfluidic system (μCE). This system incorporates a split-flow pressure injection of the sample into a microfluidic system made from PDMS and a short (∼20 cm) length of fused-silica capillary as a separation unit. The on-capillary detection was carried out by fiber optic spectrometry. A mixture of six cephalosporin antibiotics was separated in the μCE system and the obtained results were compared to those achievable by conventional CE. The six components could be separated within 8.5 min with the number of theoretical plates around 10 000.

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

“…The device showed excellent reproducibility and good recovery of spiked vancomycin in human plasma. Koczka and Gaspar describe a fused‐silica capillary microfluidic device for determining cephalosporins. The device performed well, separating six cephalosporins in 8.5 min and was comparable to conventional CE.…”

Analysis of antibiotics by CE and their use as chiral selectors: An update

“…Conditions: 50 mM phosphate buffer, pH 6.8, U: +15 kV, λ = 270 nm, sample: 2 mg/mL cefadroxil (CFD), cefaclor (CFC), ceftazidim (CZI), cefuroxim (CFR), ceftriaxon (CTR), cefixim (CIX), CE: Leff = 22 cm, hydrodynamic injection: 50 mbar 2 s, μCE: Leff = 18 cm, split‐flow injection (2 μL → ∼400 pL). Reprinted with permission from .…”

“…Koczka et al developed a portable capillary‐assembled microfluidic system in which the injection is carried out in a microchip device but the electrophoretic separation is performed in a conventional CE capillary (Fig. A) . On‐capillary UV detection was performed using a miniaturized fiber optic.…”