Application of a colorimetric method to the determination of the protein content of commercial foods, mixed human diets and nitrogen losses in infantile diarrhoea

Cioccia, Anna María; Pérez, M.M. García; Mora, José Antonio Soberón; Römer, H; Molina, C. E. A.; Hevia, Patricio

doi:10.3109/09637489509003382

Cited by 8 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The nitrogen, phosphorus, potassium, calcium, iron and zinc concentrations were determined following the standard procedures as recommended by AOAC (1990). The protein content was estimated by multiplying the nitrogen concentration by the conversion factor of 6.25 (Cioccia et al, 1995).…”

Section: Methodsmentioning

confidence: 99%

Effect of organic and inorganic fertilizers on nutrient concentrations in plantain (Musa spp.) fruit pulp

Ndukwe¹

2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The number of fruits per bunch and nutritional quality of the fruits are important horticultural and breeding selection indices in Musa improvement programs. Three plantain hybrids ('30456-3', 'PITA 14' and '29525') and a landrace genotype, 'Agbagba', were evaluated for response to organic and inorganic fertilizers in a 4 x 3 factorial in a randomized complete block design (RCBD) in triplicate. Fruit parameters measured were fruit weight, edible proportion and pulp dry matter content; also, the concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), iron (Fe) and zinc (Zn) in fruits were determined. These parameters were measured in two cropping cycles, the plant and ratoon crops, respectively. The hybrid, '29252' had the highest pulp N, K, Ca, Fe and Zn concentrations in the plant crop. In the ratoon crop, N and P were highest in '29525' and '30456-3', while the concentrations of the other nutrients did not differ among the genotypes. The landrace, 'Agbagba', produced the heaviest fruits, accumulated the highest pulp dry matter and fresh edible proportion in both crop cycles. Although in the plant crop, the fertilizer treatment did not affect most of the pulp nutrient concentrations, the P concentration decreased by 14.29 and 118.18%, respectively when inorganic fertilizer and poultry manure was applied. The N, K and Zn concentrations, respectively, increased by 6.45, 14.55 and 62.50% with inorganic fertilizer application, while all the nutrient concentrations were lowest when no fertilizer was applied. The fresh fruit weight, pulp dry matter content and fresh edible proportion were highest when poultry manure was applied.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of organic and inorganic fertilizers on nutrient concentrations in plantain (Musa spp.) fruit pulp

Ndukwe¹

2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Los alimentos y raciones fueron muestreados para determinar la MS, MO (MS menos cenizas.-C.-a 600ºC; AOAC, 1884), PB (nitrógeno total por 6,25, Cioccia et al, 1995), FDN, FDA (Van Soest, 1994), los resultados se presentan en las tablas I y II. Las colecciones de licor ruminal se realizaron antes de ofrecer el alimento (0) y a las 3, 6, 9 y 12 horas después de ofrecido, para la medición de pH se usó directamente un pHmetro portátil al momento de recoger las muestras de licor, conservando las muestras congeladas aplicando la metodología descrita por Preston (1995) a fin de hacer el análisis posterior del nitrógeno amoniacal (Cioccia et al, 1995). La degradabilidad in vivo de MS, PB y FIDN, mediante el uso de bolsas de nylon que fueron incubadas por 48 horas en el rumen según la técnica descrita por Orskov (1982).…”

Section: Uso De Cama De Pollos Subproductos De Cereales Y Caña En Ovunclassified

Efecto de cama de pollos, subproductos de cereales y caña sobre la fisiología ruminal de ovinos.

et al. 2009

View full text Add to dashboard Cite

PALABRAS CLAVE ADICIONALES Consumo. Digestibilidad. Degradabilidad. Pulitura de arroz. Harina de subproductos de maíz. ADDITIONAL KEYWORDS Intake. Digestibility. Degradability. Rice polishing. Homini. RESUMENSe determinó el efecto sobre el consumo y digestibilidad aparente de niveles crecientes de cama de pollo (Cp) con harina gruesa de subproductos de maíz (Hm) o pulitura de arroz (Pa) y bagacillo de caña amonificado (40%, Ba), en raciones para corderos en crecimiento en confinamiento total. Se realizó un experimento en un arreglo factorial 2x4 en diseño de cuadrado latino con 8 corderos canulados en el rumen, incorporando Hm o Pa (48, 39, 30 y 21%, en ambos casos) y Cp (11,4; 20,4; 29,4 y 38,4%). Las raciones con Cp y Hm fueron más consumidas que las de Cp y Pa (10 a 12,1% del peso metabólico en materia seca (MS), para Hm vs. 7,6 a 9,3% para Pa) y para la materia orgánica (MO) 8,1 a 8,5% del peso metabólico para Hm y 5,8 a 6,4% para Pa; respecto a la digestibilidad aparente (DA) se obtuvo un 34,2 a 43% de la MS y 67,8 a 67,1% de la MO para raciones con Hm vs. 30,5 a 37,2% de la MS y 65,5 a 66,1% de la MO para raciones con Pa. Se apreció que el pH y el nitrógeno amoniacal, no alcanzaron los valores indicativos de acidosis ni de intoxicación con amoniaco, lo cual se correspondió con valores normales de su fisiología sanguínea para raciones con Cp y Hm. Los resultados indican que Hm y Ba en raciones con Cp permitieron un consumo y digestibilidad de los nutrientes dentro de rangos normales para corderos, sin riesgos para la salud del animal, lo cual no ocurrió con las raciones que contenían Pa y Cp. SUMMARYThe effect on intake and digestibility of increasing levels of poultry litter (Cp), corn byproduct meal (Hm) or rice polishing (Pa) and sugarcane ammoniated bagasse (Ba, 40%) in rations for growing lambs in total confinement was determined. A factorial arrangement 2x4 Latin square design, with 8 sheep cannulated in rumen, was used, incorporating Hm or Pa (48, 39, 30 and 21% in both cases) and Cp (11.4, 20.4, 29.4, and 38.4%). Dry matter intake was higher in rations with Cp and Hm with respect to Cp and Pa (10-12.1% of metabolic weight for Hm vs. 7.6-9.3% for Pa) and for organic matter (MO) intake from 8.1 to 8.5% of metabolic weight for Hm and 5.8% to 6.4% for Pa; the apparent digestibility (DA) of dry matter was 34.2-43% and 67.8-67.1% for MO in rations with Hm vs. 30.5-37.2% and 65.5-66.1% respectively for Pa. The ruminal pH and amoniacal nitrogen levels, were not indicative of acidosis or ammonia poisoning, which was consistent with normal blood physiology for rations with Cp and Hm. Results indicate that Hm and Ba rations with Cp allowed consumption and digestibility of nutrients within the normal range for lambs, without risk to animal health, which did not occur with the ratios containing Pa and Cp. INTRODUCCIÓNLos sistemas de producción con rumian-

show abstract

Effect of food intake on protein quality measured in chicks by traditional or biochemical methods

Carías

Cioccia

Hevia

1998

J. Sci. Food Agric.

Self Cite

View full text Add to dashboard Cite

Previous studies have shown that the activities of the hepatic enzymes xanthine dehydrogenase and nucleoside phosphorylase, as well as the uric acid excreted, can be used to determine the quality of the protein consumed in chickens, in a short time and using a small amount of the test protein. A common observation in protein quality evaluation is that the food intake of the control animals is considerably greater than that observed in those receiving proteins of low quality. Since this can a †ect the results, this study measured the quality of garbanzo bean and black bean proteins in chickens fed these beans ad libitum, feeding the bean diets at the level observed in the controls (soy protein ] methionine) by enteral intubation or pair feeding the controls with the amount of food consumed by the chickens receiving the bean diets. In every case, protein quality was determined by protein efficiency ratio, net protein utilisation or the biochemical methods used in this study. The results showed that, when fed ad libitum, the animals assigned to the bean diets exhibited a lower food intake than the controls but, by tube feeding, food intake was made equal in both groups. Equal consumption, between these groups, was also obtained by reducing the amount of food o †ered to the controls to the levels measured in the groups assigned to the bean diets (pair feeding). These feeding strategies had a marked e †ect on growth, carcass protein content, protein catabolism and also in the results of protein quality. Thus, the control whose intake was reduced grew less, accumulated less protein in tissues and catabolised more of the protein consumed than the control fed ad libitum. In contrast, the chickens assigned to the bean diets, but whose food intake was increased by tube feeding, grew better, accumulated more protein in tissues and catabolised less protein. In general, the results of these experiments conÐrm the e †ectiveness of the biochemical methods for estimating protein quality and indicate that the protein quality of both garbanzo beans and black beans increased when it was measured at a food intake equivalent to that measured in the control animals. Since the overall purpose of this study was to evaluate techniques for protein quality determination meant to be applicable to humans nutrition rather than poultry nutrition, single proteins were used instead of combinations of proteins. Society of Chemical (1998 Industry.

show abstract

Application of a colorimetric method to the determination of the protein content of commercial foods, mixed human diets and nitrogen losses in infantile diarrhoea

Cited by 8 publications

References 12 publications

Effect of organic and inorganic fertilizers on nutrient concentrations in plantain (Musa spp.) fruit pulp

Effect of organic and inorganic fertilizers on nutrient concentrations in plantain (Musa spp.) fruit pulp

Efecto de cama de pollos, subproductos de cereales y caña sobre la fisiología ruminal de ovinos.

Effect of food intake on protein quality measured in chicks by traditional or biochemical methods

Contact Info

Product

Resources

About