Application of a Liquid Biphasic Flotation (LBF) System for Protein Extraction from Persiscaria Tenulla Leaf

Saw, Hui Shi; Sankaran, Revathy; Khoo, Kuan Shiong; Chew, Kit Wayne; Phong, Win Nee; Tang, Malcolm S.Y.; Lim, Siew Shee; Zaid, Hayyiratul Fatimah Mohd; Naushad, Mu.; Show, Pau Loke

doi:10.3390/pr8020247

Cited by 9 publications

(4 citation statements)

References 32 publications

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“…These techniques have been reported to be successfully applied for the separation of proteins from agricultural waste. Advanced LLE techniques have received positive feedback for the application of LBS, LBF, TMP, and TPP on the extraction of proteins from agricultural waste [29,[66][67][68].…”

Section: Advanced Liquid-liquid Extraction Techniques In the Recovery And Purification Of Proteins From Agricultural Wastementioning

confidence: 99%

“…The SS process is based on bubble-separation technology, where aqueous hydrophobic chemicals are adsorbed onto the bubble surface of the ascending gas stream bubble and transferred to the immiscible top phase. The incorporation of LBS and SS involves the liquid medium of the LBS phases to promote the mass transfer of biomolecules from the SS system, thus improving the efficiency of the phase formation of the immiscible liquid [67].…”

Section: Liquid Biphasic Flotationmentioning

confidence: 99%

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Latest Advances in Protein-Recovery Technologies from Agricultural Waste

Yusree

Peter

Nor

et al. 2021

Foods

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In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.

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Section: Advanced Liquid-liquid Extraction Techniques In the Recovery And Purification Of Proteins From Agricultural Wastementioning

confidence: 99%

Section: Liquid Biphasic Flotationmentioning

confidence: 99%

Latest Advances in Protein-Recovery Technologies from Agricultural Waste

Yusree

Peter

Nor

et al. 2021

Foods

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“…The global protein demand is, thus, projected to quadruple by 2050 [ 2 , 3 , 4 ]. Proteins from animals and plants are the most common sources of dietary protein [ 1 , 5 , 6 , 7 ]; although, it is claimed that animal-based protein sources are more popular [ 8 ]. However, plant-based protein sources are gaining ground as, compared to animal protein sources, they are more renewable, sustainable, cheaper, environment friendly and healthier [ 8 , 9 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Soybean Protein Extraction with Ammonium Hydroxide (NH4OH) Using Response Surface Methodology

Bello

Adeniyi

Mukaila

et al. 2023

Foods

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Plants have been recognized as renewable and sustainable sources of proteins. However, plant protein extraction is challenged by the plant’s recalcitrant cell wall. The conventional extraction methods make use of non-reusable strong alkali chemicals in protein-denaturing extraction conditions. In this study, soy protein was extracted using NH4OH, a weak, recoverable, and reusable alkali. The extraction conditions were optimized using response surface methodology (RSM). A central composite design (CCD) with four independent variables: temperature (25, 40, 55, 70, and 85 °C); NH4OH concentration (0.5, 1, and 1.5%); extraction time (6, 12, 18, and 24 h) and solvent ratio (1:5, 1:10, 1:15 and 1:20 w/v) were used to study the response variables (protein yield and amine concentration). Amine concentration indicates the extent of protein hydrolysis. The RSM model equation for the independent and response variables was computed and used to create the contour plots. A predicted yield of 64.89% protein and 0.19 mM amine revealed a multiple R-squared value of 0.83 and 0.78, respectively. The optimum conditions to obtain the maximum protein yield (65.66%) with the least amine concentration (0.14 Mm) were obtained with 0.5% NH4OH concentration, 12 h extraction time, and a 1:10 (w/v) solvent ratio at 52.5 °C. The findings suggest that NH4OH is suitable to extract soybean protein with little or no impact on protein denaturation.

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“…The nanofiltration membrane technology developed showed a comparable fatty acid methyl ester (FAME) composition to that of commercial lipase, which improves its applicability and scalability. The last featured study shows the application of a Liquid Biphasic Flotation (LBF) system to extract proteins from Persicaria tenella leaf [6]. The efficient, environmental friendly, and cost-effective liquid separation method showed a good reliability with a high protein recovery and separation efficiency.…”

mentioning

confidence: 99%