Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms

Bodour, Adria A.; Maier, Raina M.

doi:10.1016/s0167-7012(98)00031-1

Cited by 380 publications

(241 citation statements)

References 17 publications

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“…Pure cultures were grown on M9 minimal salt broth added with glucose (2% w/v) olive oil, paraffin or sucrose (1% w/v), and incubated at 120 rpm for five days, according to Bodour and Miller (4). After growth, biosurfactant production was tested.…”

Section: Growth Conditionsmentioning

confidence: 99%

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Viramontes-Ramos¹,

M²,

Ballinas-Casarrubias³

et al. 2010

Braz. J. Microbiol.

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Petroleum-derived hydrocarbons are among the most persistent soil contaminants, and some hydrocarbondegrading microorganisms can produce biosurfactants to increase bioavailability and degradation. The aim of this work was to identify biosurfactant-producing bacterial strains isolated from hydrocarboncontaminated sites, and to evaluate their biosurfactant properties. The drop-collapse method and minimal agar added with a layer of combustoleo were used for screening, and positive strains were grown in liquid medium, and surface tension and emulsification index were determined in cell-free supernantant and cell suspension. A total of 324 bacterial strains were tested, and 17 were positive for the drop-collapse and hydrocarbon-layer agar methods. Most of the strains were Pseudomonas, except for three strains (Acinetobacter, Bacillus, Rhodococcus). Surface tension was similar in cell-free and cell suspension measurements, with values in the range of 58 to 26 (mN/m), and all formed stable emulsions with motor oil (76-93% E 24 ). Considering the variety of molecular structures among microbial biosurfactants, they have different chemical properties that can be exploited commercially, for applications as diverse as bioremediation or degradable detergents.

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Section: Growth Conditionsmentioning

confidence: 99%

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Viramontes-Ramos¹,

M²,

Ballinas-Casarrubias³

et al. 2010

Braz. J. Microbiol.

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“…The criterion used for selecting biosurfactant producers is the ability to reduce the surface tension below 40 mN m-1 [16,9] while a criterion cited for emulsionstabilizing capacity is the ability to maintain at least 50% of the original emulsion volume 24 h after formation [56]. Strains were cultivated on the standard medium and screening of biosurfactant producing colonies was performed using the qualitative drop-collapse test described by Jain et al [33] after being modified by Bodour and Maier [9]. Motor oil, corn oil and olive oil also were evaluated for use in this test.…”

Section: Morphological Biochemical and Physiological Characterizationmentioning

confidence: 99%

Isolation and characterization of halophilic Archaea able to produce biosurfactants

Kebbouche-Gana

Gana

Khemili

et al. 2009

J Ind Microbiol Biotechnol

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Halotolerants microorganisms able to live in saline environments, offer a multitude of actual or potential applications in various fields of biotechnology. This is why some strains of Halobacteria from an Algerian culture collection were screened for biosurfactant production in a standard medium using the qualitative drop-collapse test and emulsification activity assay. Five of the Halobacteria strains reduced the growth medium surface tension below 40mNm-1 and two of them exhibited high emulsion-stabilising capacity. Diesel oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 35% sodium chloride or up to 25% ethanol in the aqueous phase. Emulsions were stable to three cycles of freezing and thawing. The components of the biosurfactant were determined; it contains sugar, protein and lipid. The two Halobacteria strains with enhanced biosurfactants producers designed strain A21 and strain D21 were selected to identify by phenotypic, biochemical characteristics and by partial 16S rRNA gene sequencing. The strains have Mg2+and salt growth requirements are always above 15% (w/v) salts with an optimal concentration of 15% to 20%. Analyses of partial 16S rRNA gene sequences of the two strains suggested that they were halophiles belonging to genera of the family Halobacteriaceae, Halovivax (strain A21) and Haloarcula (strain D21). To our knowledge, this a first report of biosurfactant production at such a high salt concentration . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1Corresponding author (e-mail: kebbouchesalima@yahoo.fr). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 Linking powered by eXtyles *Manuscript Click here to download Manuscript: cleaned_up_manuscrit.doc Click here to view linked References AbstractHalotolerants microorganisms able to live in saline environments, offer a multitude of actual or potential applications in various fields of biotechnology. This is why some strains of Halobacteria from an Algerian culture collection were screened for biosurfactant production in a standard medium using the qualitative drop-collapse test and emulsification activity assay. Five of the Halobacteria strains reduced the growth medium surface tension below 40mNm -1 and two of them exhibited high emulsion-stabilising capacity. Diesel oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 35% sodium chloride or up to 25% ethanol in the aqueous phase. Emulsions were stable to three cycles of freezing and thawing. The components of the biosurfactant were determined; it contains sugar, protein and lipid. The two H...

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“…Biosynthesis of biosurfactants was also explored by means of drop collapse and oil spreading tests 30 . Selection of these conventional methods has been attributed to their advantages like counting ease, low cost and quick execution 42,43 .…”

Section: Discussionmentioning

confidence: 99%

“…Plaza et al 22 proposed the drop collapse technique as a quantitative method to assess biosurfactant production 30 . However the present study involves the use of this technique for qualitative estimation.…”

Section: Drop Collapse Test Oil Spreading Assay and Emulsification Amentioning

confidence: 99%

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

Mulani¹,

Fulke²,

DeSouza³

et al. 2017

Current Science

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The marine environment is open to large sources of toxic organic waste in the form of accidental oil spills. Therefore, it is important to study microbial degradation processes that help reduce the damage caused to the environment. Universally, oil spills produce enormous public anxiety and highlight the need for costeffective, indigenous and environmentally acceptable bioremediation technologies. In recent times, advanced remedial techniques have been opted, such as solidifying, skimming, controlled burning and bioremediation. The present study aimed to isolate crude oil-degrading marine bacteria from Vadinar coastal area of Gujarat, India. Among seven isolates, three potential bacterial strains were chosen for crude oil and petroleum hydrocarbon (PHC) degradation, which were analysed by UV spectrophotometric and fluorometric analysis. These bacterial cultures were verified by 16S rRNA gene sequencing and identified as Bacillus species. Phylogenetic analysis was carried out to confirm the evolutionary relationship with existing oil-degrading species. In the present study, drop collapse, oil spreading and emulsification assay were performed to detect biosurfactant production. Bacillus sp. NM1 KT354277 was capable of degrading 50% of PHCs at the end 72 h for one week under rotary incubation in ONR7a medium. Among the studied strains, Bacillus sp. NM3 KT354278 showed promising lipase activity, viz. 60.72 and 61.19 U ml -1 for 2% of olive oil and tributyrin respectively. Thus, the present study explores indigenous marine isolates that could be utilized as a potential alternative for oilspill remediation in future.

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Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms

Cited by 380 publications

References 17 publications

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Isolation and characterization of halophilic Archaea able to produce biosurfactants

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

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