Application of a programmed semi-automated Ulva pertusa bioassay for testing single toxicants and stream water quality

Lee, Hojun; Park, Ji‐Hae; Shin, Kisik; Depuydt, Stephen; Choi, So‐Yeon; Saeger, Jonas De; Han, Taejun

doi:10.1016/j.aquatox.2020.105426

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…U. australis , previously known as Ulva pertusa , is an ecologically and economically significant widely distributed macroalga. This macroalga forms ‘green tides’ in coastal areas of Asia and the Mediterranean and its sensitivity to pollutants makes it a model species for water quality assessment [ 18 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. U. australis is also a valuable food source in many Asian countries [ 45 ] and exhibits algicidal and therapeutic properties attributed to its bioactive compounds [ 46 , 47 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Blue Light and Water Turbulence on the Growth of the Green Macroalga Ulva australis (Chlorophyta)

Lee,

Depuydt,

Shin

et al. 2024

Plants

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Macroalgal growth and yield are key to sustainable aquaculture. Although light and water turbulence are two important factors that affect algal productivity, research on their interaction is limited. Therefore, in this study, we investigated the effects of different wavelengths of light and the presence or absence of water turbulence on the growth of the green macroalga Ulva australis. Water turbulence was found to enhance the growth of U. australis irrespective of photosynthetic performance, but only in blue light cultures. The quantum dose of blue light required to induce 50% growth promotion was 1.02 mol m−2, which is comparable to the reported values for cryptochrome-mediated effects in other macroalgae. The combined effect of blue light and water turbulence led to the accumulation of photosynthesis-related proteins that support plastid differentiation and facilitate efficient photosynthesis and growth. Our findings thus highlight the potential of harnessing blue light and water turbulence to maximise macroalgal cultivation for sustainable and profitable algal aquaculture.

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Section: Introductionmentioning

confidence: 99%

Interactive Effects of Blue Light and Water Turbulence on the Growth of the Green Macroalga Ulva australis (Chlorophyta)

Lee,

Depuydt,

Shin

et al. 2024

Plants

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“…Under these conditions, algae also exhibit a heightened production of reactive oxygen species (ROS), which results in the cell membrane being damaged. This widespread and metal-exposure-driven DNA damage culminates in the development of metabolic disorders, which then eventually results in the death of the organism [ 11 , 12 , 13 , 14 , 15 , 16 ]. Therefore, it is necessary to create effective monitoring and management strategies, especially within the context of maintaining the ecological integrity of marine ecosystems in the ongoing Anthropocene.…”

Section: Introductionmentioning

confidence: 99%

“…U. australis is an ideal model organism for ecotoxicity tests; for example, this species has been used to develop a test that has been applied to 75 different environmental samples containing contaminants such as metals, volatile organic compounds, herbicides, oils, dispersants, and slag waste [ 16 , 21 ]. The test measures the inhibition of the reproduction on the margins of the thallus by quantifying the colour changes exhibited by the thallus (i.e., caused by the release of reproductive cells) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…The test measures the inhibition of the reproduction on the margins of the thallus by quantifying the colour changes exhibited by the thallus (i.e., caused by the release of reproductive cells) [ 20 ]. Ecotoxicity testing using U. australis has some advantages over conventional chemical techniques, namely, ease of use, sensitivity, cost, efficiency, environmental relevance, repeatability, and reproducibility [ 16 , 19 , 20 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Metal Toxicity across Different Thallus Sections of the Green Macroalga, Ulva australis

Lee

Kim

Depuydt

et al. 2023

Toxics

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We aimed to identify functional differences between different sections of the thallus of Ulva australis and develop tissue-endpoint combinations to assess the toxicity of six metals (i.e., Ag, As, Cd, Cr, Cu, and Ni). EC50 values for these metals in three sections of the thallus of Ulva were obtained for multiple endpoints: relative growth rate (RGR), chlorophyll a fluorescence, pigment contents, and the expression of the photosynthesis-related gene, rbcL. The responses of the endpoints varied across the respective thallus sections; overall, the most toxic metals were Ag and Cu. These endpoints were the best for evaluating metal toxicity: ETRmax of the middle thallus sections for Ag toxicity; RGR of the middle thallus section for As and Cd; ETRmax of the marginal thallus section for Cr; Chl b contents of the marginal thallus section for Cu; RGR of the basal thallus section for Ni. The EC50 values for the inhibition of ETRmax in middle (0.06 mg∙L−1) and Chl b in the marginal thallus sections (0.06 mg∙L−1) were all lower than those of the quality standard for wastewater discharge values of Ag and Cu in Republic of Korea and the US, pointing to the suitability of U. australis-based endpoints for risk assessment.

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“…Some applications, for instance ImageJ, which have a user interface, provide an accurate and flexible method for automating the analysis of digital photographs of laboratory microcosms. These applications can detect, count, and measure organisms, such as collembola, ants, nematodes, and daphnias, moving on a fixed but heterogeneous substrate (Mallard et al 2013 ) or detect changes in the color of Ulva pertusa macroalgae (Lee et al 2020 );…”

Section: Introductionmentioning

confidence: 99%

Automated software for counting and measuring Hyalella genus using artificial intelligence

Pineda-Alarcón,

Zuluaga,

Ruíz

et al. 2023

Environ Sci Pollut Res

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Amphipods belonging to the Hyalella genus are macroinvertebrates that inhabit aquatic environments. They are of particular interest in areas such as limnology and ecotoxicology, where data on the number of Hyalella individuals and their allometric measurements are used to assess the environmental dynamics of aquatic ecosystems. In this study, we introduce HyACS, a software tool that uses a model developed with the YOLOv3’s architecture to detect individuals, and digital image processing techniques to extract morphological metrics of the Hyalella genus. The software detects body metrics of length, arc length, maximum width, eccentricity, perimeter, and area of Hyalella individuals, using basic imaging capture equipment. The performance metrics indicate that the model developed can achieve high prediction levels, with an accuracy above 90% for the correct identification of individuals. It can perform up to four times faster than traditional visual counting methods and provide precise morphological measurements of Hyalella individuals, which may improve further studies of the species populations and enhance their use as bioindicators of water quality.

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Application of a programmed semi-automated Ulva pertusa bioassay for testing single toxicants and stream water quality

Cited by 8 publications

References 33 publications

Interactive Effects of Blue Light and Water Turbulence on the Growth of the Green Macroalga Ulva australis (Chlorophyta)

Interactive Effects of Blue Light and Water Turbulence on the Growth of the Green Macroalga Ulva australis (Chlorophyta)

Metal Toxicity across Different Thallus Sections of the Green Macroalga, Ulva australis

Automated software for counting and measuring Hyalella genus using artificial intelligence

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