Application of a validated HPLC-PDA method for the determination of melatonin content and its release from poly(lactic acid) nanoparticles

Abstract

Melatonin is a natural hormone and with the advancement of age its production declines and thereby may result in some neurological disorders. Exogenous administration of melatonin has been suggested as a neuroprotective agent. Due to its low oral bioavailability, the loading of melatonin in polymeric nanoparticles could be an important tool to effectively use exogenous melatonin. The quantification of the incorporated drug within polymeric nanoparticles is an important step in nanoparticles characterization. An analytical method using high performance liquid chromatography equipped with photodiode array detector (HPLC-PDA) was developed and validated for melatonin determination in poly (lactic acid) nanoparticles obtained by a single emulsion-solvent evaporation technique. The melatonin in vitro release profile also was determined by the HPLC method. Mobile phase consisted of acetonitrile: water (65:35, v/v) pumped at a flow rate of 0.9 mL/min, in the isocratic mode and PDA detector was set at 220 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. Analytical curve was linear over the concentration range of 10–100 μg/mL, and limits of detection and quantification were 25.9 ng/mL and 78.7 ng/mL, respectively. The mean recovery for melatonin was 100.47% (RSD = 1.25%, n = 9). In the intra- and inter-assay, the coefficient of variation was less than 2%. Robustness was proved performing changes in mobile phase, column temperature and flow rate. The method was suitable for the determination of melatonin encapsulation efficiency in poly(lactic acid) nanoparticles and for the evaluation of melatonin in vitro release profile.

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF BENIDIPINE HYDROCHLORIDE BY USING QUALITY BY DESIGN (QbD) APPROACH

“…For system suitability % RSD of absorbance of replicate solutions is should not more than 2 [18]. The results obtained was meets the system suitability requirements, this indicates that the system was suitable for the analysis [19].…”

Simultaneous GC-FID Quantification of Main Components of Rosmarinus officinalis L. and Lavandula dentata Essential Oils in Polymeric Nanocapsules for Antioxidant Application

“…However, EO are highly volatile and can decompose easily due to direct exposure to heat, moisture, light, or oxygen [18]. Nanoencapsulation represents a feasible and efficient alternative to overcome these drawbacks since it would provide stability to the active substance [19], protection against oxidation [20], retention of volatile compounds, and reduction of side effects [20, 21]. In recent years, there has been growing interest in the development of polymeric nanoparticles, particularly nanocapsules (NC), as systems for the administration of lipophilic bioactive components [22], representing a new form of topical administration of EO with a preventive and/or therapeutic antioxidant effect.…”

Melatonin-Loaded Nanocarriers: New Horizons for Therapeutic Applications

“…Using HPLC equipped with photodiode array detector, melatonin was validated and determined in poly-lactic acid (PLA) NPs [126]. The in vitro release of melatonin was analyzed in mobile phase consisting of acetonitrile: water (65:35, v/v) at a flow rate of 0.9 mL/min, using an isocratic mode and PDA detector at 220 nm.…”