Application of Adult and Pluripotent Stem Cells in Interstitial Cystitis/Bladder Pain Syndrome Therapy: Methods and Perspectives

Dayem, Ahmed Abdal; Kim, Kyeongseok; Lee, Soo Bin; Kim, Aram; Cho, Ssang-Goo

doi:10.3390/jcm9030766

Cited by 11 publications

(16 citation statements)

References 218 publications

(296 reference statements)

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“…Only a single study has been performed previously, reporting the expression of SP receptor, NK-1R in human dental pulp. This study proved that SP receptor expression was present in all human pulp tissue samples, and there was an intense expression of NK-1R in the group of pulps from teeth clinically diagnosed with acute irreversible pulpitis [ 4 ]. Cytokine interleukin 1 (IL-1) has been observed to be main player in the periodontitis [ 26 ].…”

Section: Discussionmentioning

confidence: 78%

“…To obtain pulp sample from group 1, access opening was made and pulp extirpation was done using a no. 15 barbed broach [ 4 ]. Immediately after the extirpation, the tissue was placed in 10% neutral buffered formalin, embedded in paraffin, and cut with a microtome to a thickness of 3-4 μ m. For each tissue sample, three different depths of cut were made, which were first stained with Hematoxylin-Eosin (H&E).…”

Section: Methodsmentioning

confidence: 99%

“…SP is abundantly present in the fibers that innervate the dentin and dental pulp. As the inflammatory processes begin, the amount of SP released by each sensory fiber is further increased, which perpetuates the vicious circle that is underlying inflammation [ 4 ]. SP binds to its receptor NK-1R preferentially and exerts all its biological actions by binding to this high-affinity G-protein-coupled receptor.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Neurokinin-1 Receptor Expression Is Associated with Human Dental Pulp Inflammation and Pain Severity

Mehboob

Hassan

Gilani

et al. 2021

BioMed Research International

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Substance P (SP) is a peptide involved in many biological processes, including nociception and inflammation. SP has a high affinity for its receptor neurokinin-1 (NK-1R). SP/NK-1R complex plays a major role in the interactions going on during the onset of dental pain and inflammation. Objective. To identify the expression of NK-1R in healthy and inflamed human dental pulp, as well as to identify any association with severity of dental pain. Methods. This case-control study included ten irreversibly inflamed samples of dental pulp, which were extirpated from patients presenting with chief complaint of dental pain due to caries. Ten healthy pulps, extirpated from those teeth which were indicated for extraction due to orthodontic reasons, were used as the control group. Visual analog scale (VAS) and modified McGill Pain Questionnaire were used to assess the characteristic and severity of pain. Immunohistochemical study was performed using monoclonal antibodies against NK-1R. Results. The results showed that the NK-1R was expressed intensely in patients with higher pain score. The mean pain score in cases was 7.0 ± 2.0 . The healthy dental pulps had negative or mild NK-1R staining of +1 intensity. The NK-1R score in cases was 2.4 ± 0.516 and 0.2 ± 0.4216 in controls. There was significant difference in NK-1R score between both groups ( p value <0.05). There was a strong positive correlation between the pain score and NK-1R expression score. As the pain increased, the NK-1R expression score was also increased (0.95 ∗ ∗ , p value 0.000). Conclusions. NK-1R is overexpressed in inflamed dental pulp. SP/NK-1R modulation may provide a novel approach for the treatment of pulpal inflammation and pain.

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Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced Neurokinin-1 Receptor Expression Is Associated with Human Dental Pulp Inflammation and Pain Severity

Mehboob

Hassan

Gilani

et al. 2021

BioMed Research International

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“…Stem cells (SCs), including adult stem cells and pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells, possess the abilities of self-renewal, proliferation, and differentiation into various cell types. The therapeutic effects of SCs have been extensively researched and preclinically trialed in many diseases, including IC/BPS [ 81 ]. To date, SCs of different origins have been tested in animal models of IC/BPS, including human umbilical cord blood, dental pulp, and adipose tissue.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review of Therapeutic Approaches Used in Experimental Models of Interstitial Cystitis/Bladder Pain Syndrome

et al. 2021

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Interstitial cystitis/bladder pain syndrome (IC/BPS) is a multifactorial, chronic bladder disorder with limited therapeutic options currently available. The present review provides an extensive overview of therapeutic approaches used in in vitro, ex vivo, and in vivo experimental models of IC/BPS. Publications were identified by electronic search of three online databases. Data were extracted for study design, type of treatment, main findings, and outcome, as well as for methodological quality and the reporting of measures to avoid bias. A total of 100 full-text articles were included. The majority of identified articles evaluated therapeutic agents currently recommended to treat IC/BPS by the American Urological Association guidelines (21%) and therapeutic agents currently approved to treat other diseases (11%). More recently published articles assessed therapeutic approaches using stem cells (11%) and plant-derived agents (10%), while novel potential drug targets identified were proteinase-activated (6%) and purinergic (4%) receptors, transient receptor potential channels (3%), microRNAs (2%), and activation of the cannabinoid system (7%). Our results show that the reported methodological quality of animal studies could be substantially improved, and measures to avoid bias should be more consistently reported in order to increase the value of preclinical research in IC/BPS for potential translation to a clinical setting.

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“…Stem cells, including embryonic stem cells (ESCs), adult stem cells (ASCs) and induced pluripotent stem cells (iPSCs), have increasingly emerged as research hot spots due to their roles in sustaining tissue homeostasis and regeneration and promoting tissue repair and responses ( Daley, 2015 ; Dayem et al, 2020 ). Although significant progress has been made in stem cell research, ESC applications are still limited by ethical and tumour formation issues.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Review of the Therapeutic Value of Urine-Derived Stem Cells

et al. 2022

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Stem cells possess regenerative powers and multidirectional differentiation potential and play an important role in disease treatment and basic medical research. Urine-derived stem cells (USCs) represent a newly discovered type of stem cell with biological characteristics similar to those of mesenchymal stromal cells (MSCs), including their doubling time and immunophenotype. USCs are noninvasive and can be readily obtained from voided urine and steadily cultured. Based on advances in this field, USCs and their secretions have increasingly emerged as ideal sources. USCs may play regulatory roles in the cellular immune system, oxidative stress, revascularization, apoptosis and autophagy. This review summarizes the applications of USCs in tissue regeneration and various disease treatments. Furthermore, by analysing their limitations, we anticipate the development of more feasible therapeutic strategies to promote USC-based individualized treatment.

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Application of Adult and Pluripotent Stem Cells in Interstitial Cystitis/Bladder Pain Syndrome Therapy: Methods and Perspectives

Cited by 11 publications

References 218 publications

Enhanced Neurokinin-1 Receptor Expression Is Associated with Human Dental Pulp Inflammation and Pain Severity

Enhanced Neurokinin-1 Receptor Expression Is Associated with Human Dental Pulp Inflammation and Pain Severity

A Systematic Review of Therapeutic Approaches Used in Experimental Models of Interstitial Cystitis/Bladder Pain Syndrome

A Comprehensive Review of the Therapeutic Value of Urine-Derived Stem Cells

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