Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea

Jeong, Jang Hae; Wu, Chunhua; Murthy, Hosakatte Niranjana; Hahn, Eun-Joo; Paek, Kee-Yoeup

doi:10.1007/s12257-007-0142-5

Cited by 79 publications

(26 citation statements)

References 34 publications

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“…The best known in vitro culture systems for production of hydroxycinnamates are root cultures of Echinacea purpurea (L.) Moench. (Liu et al 2006;Abbasi et al 2007;Wu et al 2007;Jeong et al 2009). According to Pellati et al (2004), total phenolics content in roots of E. purpurea plants was 2.3% DW.…”

Section: Resultsmentioning

confidence: 99%

“…According to Pellati et al (2004), total phenolics content in roots of E. purpurea plants was 2.3% DW. Adventitious root cultures of E. purpurea investigated by Jeong et al (2009) accumulated 0.49% DW of CTA, 0.60% DW of 5-CQA and 2.81% DW of DCTA in the optimized culture conditions (GI 10.9). Hairy roots of the plant produced 12.9 g l -1 DW of biomass after 35-day culture.…”

Section: Resultsmentioning

confidence: 99%

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Caffeic acid derivatives from a hairy root culture of Lactuca virosa

et al. 2011

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In a search for biologically active phenolics, a hydroalcoholic extract from the hairy roots of Lactuca virosa was fractionated by chromatographical methods. The procedure led to the isolation of a substantial amount of 3,5-dicaffeoylquinic acid (3,5-DCQA)-a potent free radical scavenger. An analytical RP-HPLC separation of the hydroalcoholic extract from the hairy roots allowed identification of further hydroxycinnamates: caftaric acid (CTA), chlorogenic acid (5-CQA) and cichoric acid (DCTA), as well as small amounts of unbound phenolic acids. A time course of growth and caffeic acid derivatives accumulation in the hairy root culture was also investigated. The highest contents of the compounds in the examined roots were detected at the logarithmic phase of growth. The average content of 3,5-DCQA in the roots (ca. 2.5% DW) was at least one order of magnitude higher than that found in roots of Lactuca species and callus culture of L. virosa.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Caffeic acid derivatives from a hairy root culture of Lactuca virosa

et al. 2011

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“…6 Therefore, cell or organ cultures have emerged as valuable routes for biosynthesizing phytochemicals. 7 The main constituents of H. perforatum are flavonoids, naphthodianthrones, phloroglucinols, and xanthones. 8 Hyperforin and adhyperforin have been shown to contribute to the antidepressant activity of H. perforatum by inhibiting the reuptake of a number of neurotransmitters.…”

Section: Introductionmentioning

confidence: 99%

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Li¹,

Ding²,

Quang³

et al. 2013

Bulletin of the Korean Chemical Society

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A new compound, perforaphenonoside A (1), along with 11 known compounds (2-12) were isolated from a methanol extract of adventitious roots of Hypericum perforatum. Their chemical structures were elucidated using chemical and physical methods as well as comparison of NMR and mass spectral data with previously reported data. Their inhibition of NF-κB and activation of PPAR was measured in HepG2 cells using a luciferase reporter system. Among the compounds 3, 6, 7 and 12 inhibited NF-κB activation stimulated by TNFα in a dose-dependent manner, with IC 50 values ranging from 0.85 to 8.10 μM. Moreover, compounds 1-3, 7, 11 and 12 activated the transcriptional activity of PPARs in a dose-dependent manner, with EC 50 values ranging from 7.3 to 58.7 μM. The transactivational effects of compounds 1-3, 7, 11 and 12 were evaluated on three individual PPAR subtypes. Among them, compound 2 activated PPARα transcriptional activity, with 153.97% stimulation at 10 μM, while compounds 1, 2 and 11 exhibited transcriptional activity of PPARγ, with stimulation from 124.76% to 126.91% at 10 μM.

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“…In general, phenolics and specifically caffeic acid derivatives have high antioxidant and antimutagenic activities Tsai et al, 2012). TCEPARs have been reported to possess higher concentrations of phenolics and flavonoids (Jeong et al, 2009;Wu et al, 2008) Some studies support the view that compounds with antioxidant activity can inhibit mutations because they scavenge free radicals or induce antioxidant enzyme activity (Yen and Chen, 1995).…”

Section: Discussionmentioning

confidence: 99%

Biosafety and Toxicological Evaluation of Tissue-Cultured Echinacea purpurea Adventitious Roots

Murthy¹,

Park²,

Lee³

et al. 2015

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Echinacea purpurea (L.) Moench (purple cone flower) is an important medicinal plant; it can enhance immunity, relieve pain, and reduce inflammation, and also has hormonal, antiviral, and antioxidant effects. Adventitious root biomass of Echinacea purpurea was produced in commercial-scale bioreactors for use as a dietary supplement in the food industry and in traditional medicine. Biosafety and toxicological evaluations of tissue-cultured Echinacea purpurea adventitious roots (TCEPARs) were performed. Reverse mutation and chromosomal aberration tests showed no significant mutagenicity. Furthermore, repeated four-week oral dose tests performed in Sprague-Dawley rats did not show any notable changes in the general behavior of the rats, in the gross appearance of their internal organs, or in their mortality rate. There were no differences between the control group and the treatment group in parameters such as absolute body weight, hematology, blood chemistry, and absolute and relative organ weights. These findings indicate that TCEPARs are safe and nontoxic when consumed at an average dietary level and can be used as raw material for traditional medicine and the food industry.

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Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea

Cited by 79 publications

References 34 publications

Caffeic acid derivatives from a hairy root culture of Lactuca virosa

Caffeic acid derivatives from a hairy root culture of Lactuca virosa

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Biosafety and Toxicological Evaluation of Tissue-Cultured Echinacea purpurea Adventitious Roots

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