Application of an immunomagnetic bead ELISA based on IgY for detection of circulating antigen in urine of mice infected with Schistosoma japonicum

Lei, Jiahui; Guan, Fei; Xu, Hong; Chen, Lin; Su, Bing-tao; Zhou, Yan; Wang, Ting; Lü, Yonglong; Liu, Wenqi

doi:10.1016/j.vetpar.2011.12.017

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…[24]. and Helicobacter pylori [25], and infections by parasites such as Trichinella spiralis [26] and Schistosoma japonicum [27]. In addition, IgY has been shown to be effective in the diagnosis of tropical diseases such as dengue [28], in the detection of neoplastic cells from human mammary tumors [29,30] and when applied as serotherapy for snake poisoning [31] and as an agent to block bacterial toxins [32].…”

Section: Discussionmentioning

confidence: 99%

Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Eto

Fernandes

Moraes

et al. 2018

Fish & Shellfish Immunology

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Streptococcus agalactiae (Sta), which belongs to Lancefield group B, causes sepsis, endocarditis and bacterial meningitis in human neonates and Nile tilapia. Because the pathophysiology of Sta infection is partially similar in both species, the identification of biomarkers for the diagnosis and study of this disease is of importance for human and animal health. Therefore, in the present study, we produced an immunoglobulin Y (IgY) by immunizing laying hens with Sta proteins and evaluated its ability to detect Sta in paraffinized tilapia brain and cardiac tissue by direct immunofluorescence (IMF) and indirect immunohistochemistry (IHC). The IgY produced was effective in the diagnosis of Sta infection in Nile tilapia, justifying the use of this species as a biomodel for the study of this disease.

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Section: Discussionmentioning

confidence: 99%

Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Eto

Fernandes

Moraes

et al. 2018

Fish & Shellfish Immunology

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“…This technique allows the serum-based diagnosis of subjects infected with S. japonicum without the need for sample pretreatment and it can diagnose individuals co-infected with schistosomiasis and TB. The current scarcity of effective diagnostic tools is a dominant factor precluding the effective management of schistosomiasis (Colley et al 2014) so Detection of circulating antigens in sera of hosts infected with S. japonicum; 100% sensitivity with acute cases and 91.5% sensitivity with chronic cases was achieved 3.3% cross-reactivity with clonorchiasis but no cross-reactivity with paragonimiasis Lei et al (2011Lei et al ( , 2012 MEIA Based on Sj14-3-3 One difficulty is in identifying an active from a previous infection, as parasite-specific antibodies remain detectable long after cure, and another is the inability to quantify infection intensity.…”

Section: Immunological Methodsmentioning

confidence: 99%

“…Indeed, detection of schistosome circulating antigens (CAs) has now been shown to be an efficient method to differentiate between previous exposure and current infection. A novel immunomagnetic bead ELISA, based on IgY, has also been used for detection of circulating CAs in sera or urine of hosts infected with S. japonicum (Lei et al 2011(Lei et al , 2012 (Table 3). The purified IgY was then immobilized onto resin to immune-precipitate CAs in sera from infected subjects.…”

Section: Immunological Methodsmentioning

confidence: 99%

Vaccines and diagnostics for zoonotic schistosomiasis japonica

You

McManus

2014

Parasitology

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Schistosomiasis is one of the most prevalent, insidious and serious of the tropical parasitic diseases. Although the effective anthelmintic drug, praziquantel, is widely available and cheap, it does not protect against re-infection, drug-resistant schistosome may evolve and mass drug administration programmes based around praziquantel are probably unsustainable long term. Whereas protective anti-schistosome vaccines are not yet available, the zoonotic nature of Schistosoma japonicum provides a novel approach for developing a transmission-blocking veterinary vaccine in domestic animals, especially bovines, which are major reservoir hosts, being responsible for up to 90% of environmental egg contamination in China and the Philippines. However, a greater knowledge of schistosome immunology is required to understand the processes associated with anti-schistosome protective immunity and to reinforce the rationale for vaccine development against schistosomiasis japonica. Importantly as well, improved diagnostic tests, with high specificity and sensitivity, which are simple, rapid and able to diagnose light S. japonicum infections, are required to determine the extent of transmission interruption and the complete elimination of schistosomiasis following control efforts. This article discusses aspects of the host immune response in schistosomiasis, the current status of vaccine development against S. japonicum and reviews approaches for diagnosing and detecting schistosome infections in mammalian hosts.

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“…Thus, in using antibody-labeled MPs, the time required for analysis is expected to be less than that required by a conventional ELISA. Most literatures on magnetic particle-based ELISA were processed in tubes [ 19 , 20 ]. The washing steps were done with one tube by one tube or with commercial magnetic separators, such as fully automated multisampling separators.…”

Section: Introductionmentioning

confidence: 99%

Detection of rabbit IgG by using functional magnetic particles and an enzyme-conjugated antibody with a homemade magnetic microplate

Tsai

Liao

et al. 2015

Chemistry Central Journal

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BackgroundThe enzyme-linked immunosorbent assay (ELISA) has been used for diagnosing medical and plant pathologies. In addition, it is used for quality-control evaluations in various industries. The ELISA is the simplest method for obtaining excellent results; however, it is time consuming because the immunoreagents interact only on the contact surfaces. Antibody-labeled magnetic particles can be dispersed in a solution to yield a pseudohomogeneous reaction with antigens which improved the efficiency of immunoreaction, and can be easily separated from the unreactive substances by applying a magnetic force. We used a homemade magnetic microplate, functional magnetic particles (MPs) and enzyme-labeled secondary antibody to perform the sandwich ELISA successfully.ResultsUsing antibody-labeled MPs enabled reducing the analysis time to one-third of that required in using a conventional ELISA. The secondary antibody conjugated with horseradish peroxidase (HRP) was affinity-bound to the analyte (IgG in this study). The calibration curve was established according to the measured absorbance of the 3, 3′, 5, 5′-tetramethybezidine–HRP reaction products versus the concentrations of standard IgG. The linear range of IgG detection was 114 ng/mL–3.5 ng/mL. The limit of detection (LOD) of IgG was 3.4 ng/mL. The recovery and coefficient of variation were 100% (±7%) and 116% (±4%) for the spiked concentrations of 56.8 ng/mL and 14.2 ng/mL, respectively.ConclusionPseudohomogeneous reactions can be performed using functional MPs and a magnetic microplate. Using antibody-labeled MPs, the analysis time can be reduced to one-third of that required in using a conventional ELISA. The substrate–enzyme reaction products can be easily transferred to another microplate, and their absorbance can be measured without interference by light scattering caused by magnetic microbeads. This method demonstrates great potential for detecting other biomarkers and in biochemical applications.Graphical AbstractA magnetic ELISA with convenient magnetic microplate.Electronic supplementary materialThe online version of this article (doi:10.1186/s13065-015-0088-1) contains supplementary material, which is available to authorized users.

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Application of an immunomagnetic bead ELISA based on IgY for detection of circulating antigen in urine of mice infected with Schistosoma japonicum

Cited by 15 publications

References 44 publications

Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

Vaccines and diagnostics for zoonotic schistosomiasis japonica

Detection of rabbit IgG by using functional magnetic particles and an enzyme-conjugated antibody with a homemade magnetic microplate

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