Application of Atomic Force Microscopy to Observation of Marine Bacteria

Nishino, Tatsuya; Ikemoto, Eiko; Kogure, Kazuhiro

doi:10.1023/b:joce.0000038328.54339.e4

Cited by 36 publications

(32 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synechococcus High-resolution imaging of pelagic bacteria by AFM F Malfatti et al cells were also either cocci or rods (Figures 1c and d) (indicating pleiomorphism that might be related to a difference in light regime (Ahlgren and Rocap, 2006;Palenik et al, 2006) and/or grazing pressure (Christaki et al, 1999(Christaki et al, , 2005). Previous scanning electron microscope and TEM imaging of marine bacteria (Costerton et al, 1981) (Sieburth, 1975) (Heissenberger et al, 1996), as well as recent AFM studies of marine bacteria (Nishino et al, 2004) (Seo et al, 2007), reported shapes similar to those in this study. As cell shape has adaptive value (Cabeen and Jacobs-Wagner, 2005), one might speculate that, despite their varied biogeochemical functions in ocean ecosystems, bacteria maintain few common shapes that are optimized for survival in the pelagic environment.…”

Section: Bacterial Surface and Morphologysupporting

confidence: 88%

“…Furthermore, AFM resolution in XY depends on how thin and sharp the probe is, and this probe dilation effect tends to cause overestimations in XY measurements (Wong et al, 2007). This point has been discussed for marine bacteria (Nishino et al, 2004), but the degree of overestimation for the natural High-resolution imaging of pelagic bacteria by AFM F Malfatti et al bacterial population is unknown. There is need to intercalibrate (probe specific) AFM measurements of marine bacteria by establishing size standards that are representative in size, shape, surface topography and chemical activity to marine bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Height reduction of marine bacteria due to sample processing was observed previously by Nishino et al (2004), but its quantitative significance for estimates of bacterial biomass and C pools in the ocean has not been made. We propose to use the formula High-resolution imaging of pelagic bacteria by AFM F Malfatti et al…”

Section: Height-biovolume Relationshipmentioning

confidence: 93%

“…This formula has recently been proposed in an AFM study on marine bacteria (Nishino et al, 2004). It requires measuring W, L and Z.…”

Section: Methodsmentioning

confidence: 99%

“…These are versatile tools that allow 3D high-resolution imaging in vivo, in natural liquid environmental conditions (Dufrene, 2002(Dufrene, , 2008. AFM has been used in microbial oceanography by Kogure's group (Nishino et al, 2004;Seo et al, 2007). Nishino et al studied the size of marine bacteria in natural assemblages and in cultures.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

High-resolution imaging of pelagic bacteria by Atomic Force Microscopy and implications for carbon cycling

2009

View full text Add to dashboard Cite

In microbial oceanography, cell size, volume and carbon (C) content of pelagic bacteria and archaea ('bacteria') are critical parameters in addressing the in situ physiology and functions of bacteria, and their role in the food web and C cycle. However, because of the diminutive size of most pelagic bacteria and errors caused by sample fixation and processing, an accurate measurement of the size and volume has been challenging. We used atomic force microscopy (AFM) to obtain highresolution images of pelagic bacteria and Synechococcus. We measured the length, width and height of live and formalin-fixed pelagic bacteria, and computed individual cell volumes. AFM-based measurements were compared with those by epifluorescence microscopy (EFM) using 4 0 ,6-diamidino-2-phenylindole (DAPI). The ability to measure cell height by AFM provides methodological advantage and ecophysiological insight. For the samples examined, EFM (DAPI)-based average cell volume was in good agreement (1.1-fold) with live sample AFM. However, the agreement may be a fortuitous balance between cell shrinkage due to fixation/drying (threefold) and Z-overestimation (as EFM does not account for cell flattening caused by sample processing and assumes that height ¼ width). The two methods showed major differences in cell volume and cell C frequency distributions. This study refines the methodology for quantifying bacteria-mediated C fluxes and the role of bacteria in marine ecosystems, and suggests the potential of AFM for individual cell physiological interrogations in natural marine assemblages.

show abstract

Section: Bacterial Surface and Morphologysupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Height-biovolume Relationshipmentioning

confidence: 93%

“…This formula has recently been proposed in an AFM study on marine bacteria (Nishino et al, 2004). It requires measuring W, L and Z.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High-resolution imaging of pelagic bacteria by Atomic Force Microscopy and implications for carbon cycling

2009

View full text Add to dashboard Cite

show abstract

Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

Truong

Rundell

Lapovok

et al. 2009

Appl Microbiol Biotechnol

106

View full text Add to dashboard Cite

The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 microm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 microm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 microm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

show abstract

Surface selection, adhesion, and retention behavior of marine bacteria on synthetic organic surfaces using self-assembled monolayers and atomic force microscopy

et al. 2009

View full text Add to dashboard Cite

Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected model surfaces-terminated by CH3, OH, NH2, COOH, OH-terminated oligo (ethylene glycol), and methyl-terminated oligo(ethylene glycol)-are characterized by contact angle measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force microscopy images of adhered bacteria showed very different fingerprints on the different surfaces

show abstract

Application of Atomic Force Microscopy to Observation of Marine Bacteria

Cited by 36 publications

References 22 publications

High-resolution imaging of pelagic bacteria by Atomic Force Microscopy and implications for carbon cycling

High-resolution imaging of pelagic bacteria by Atomic Force Microscopy and implications for carbon cycling

Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

Surface selection, adhesion, and retention behavior of marine bacteria on synthetic organic surfaces using self-assembled monolayers and atomic force microscopy

Contact Info

Product

Resources

About