Application of computational methods in the analysis of pesticide target-site and resistance mechanisms

Banba, Shinichi

doi:10.1584/jpestics.j21-01

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…Studies suggest that the target site of meta-diamides such as broflanilide differs from that of conventional noncompetitive inhibitors such as fipronil. Furthermore, meta-diamides are effective against fipronil-resistant pests that carry target-site mutations [ 34 ]. Cyproflanilide is a potent insect GABA receptor antagonist classified as a member of group 30 by the IRAC.…”

Section: Discussionmentioning

confidence: 99%

The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin

et al. 2023

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Background Cyproflanilide is a novel chemical that is already undergoing insecticide registration in China and has been categorized as a member of group 30 by the IRAC. Since it was first detected in 2019, the fall armyworm (FAW), Spodoptera frugiperda, has become a serious pest in China. Our laboratory and field efficacy trials indicated that cyproflanilide exhibits high larvicidal activity against FAW. However, the effect of cyproflanilide against FAW remains unknown. And it is worth exploring further before the cyproflanilide becomes commercially available. Results We found larvae exposed to cyproflanilide had significantly shorter body length and higher death rates compared to control larvae. Additionally, we found surviving larvae had a significantly longer developmental period compared to control larvae. The potential molecular mechanisms of cyproflanilide against FAW were investigated using comparative transcriptomic analyses on larval samples subjected to three insecticide treatments, including cyproflanilide and two other commonly used insecticides against FAW in China, chlorantraniliprole and avermectin. We found that several subunits of the γ-aminobutyric acid receptor (GABAR), a possible target protein of cyproflanilide, were significantly up-regulated at the transcriptional level during cyproflanilide-induced stress. Additionally, between the control and cyproflanilide-treated samples, we identified 131 differentially expressed genes (DEGs) associated with detoxification metabolism. Of these, we found four P450 genes that were significantly up-regulated under cyproflanilide stress but were not DEGs when exposed to chlorantraniliprole and avermectin, or 23 other pesticides from previous reports. Furthermore, we discovered an interesting gene aggregation region for insect cuticle proteins (CPs) on the 18th chromosome, which is likely related to FAW cross-resistance to cyproflanilide and avermectin. Conclusions Our results contribute to a greater understanding of the mechanisms by which cyproflanilide affects FAW. Additionally, we identified the similarities and differences in transcriptomic profiling of FAW between the novel insecticide cyproflanilide and two other commonly used insecticides.

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Section: Discussionmentioning

confidence: 99%