Application of dental pulp stem cells in oral maxillofacial tissue engineering

Liu, Peng; Zhang, Yingxin; Ma, Yujie; Tan, Shuang; Ren, Bingyi; Liu, Shitao; Dai, Huanyan; Xu, Zhimin

doi:10.7150/ijms.68494

Cited by 27 publications

(19 citation statements)

References 104 publications

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“…DPSCs with self-renewing, proliferation, and differentiation properties can be a favorable substitute for tissue repair [ 8 , 9 , 11 ]. They have been used in regenerative medicine [ 32 ], including pulp regeneration [ 33 ], oral maxillofacial tissue engineering [ 34 ], and many other diseases [ 35 ], but there are still limitations in their clinical application. Therefore, an effective method to be non-invasive and to accurately track the survival and migration of these grafted cells can open a door in cell transplantation.…”

Section: Discussionmentioning

confidence: 99%

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Jalli

Mehrabani

Zare

et al. 2023

JCM

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To assess in vitro and in vivo tracking of iron oxide labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI), rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine to facilitate the internalization of these nanoparticles. Cell proliferation, viability, differentiation, and apoptosis were investigated. Prussian blue staining and MRI were used to trace transfected labeled cells. DPSCs were a morphologically spindle shape, adherent to culture plates, and positive for adipogenic and osteogenic inductions. They expressed CD73 and CD90 markers and lacked CD34 and CD45. Iron oxide labeling and transfection with lipofectamine in DPSCs had no toxic impact on viability, proliferation, and differentiation, and did not induce any apoptosis. In vitro and in vivo internalization of iron oxide nanoparticles within DPSCs were confirmed by Prussian blue staining and MRI tracking. Prussian blue staining and MRI tracking in the absence of any toxic effects on cell viability, proliferation, differentiation, and apoptosis were safe and accurate to track DPSCs labeled with iron oxide and transfected with lipofectamine. MRI can be a useful imaging modality when treatment outcome is targeted.

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Section: Discussionmentioning

confidence: 99%

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Jalli

Mehrabani

Zare

et al. 2023

JCM

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“…These tissues are closely related to each other and support the maxillofacial structure and function basis of the oromandibular system. 79 Once a defect occurs, it not only affects the patient’s physiological functions, such as chewing and pronunciation, but also has a serious impact on the patient’s psychological health. However, the self-repair ability of oral and maxillofacial hard tissues is limited, and repair of these hard tissues mainly relies on synthetic materials for alternative repair of the defective area.…”

Section: Biomedical Applications Of Pda Hydrogel In Oral Tissuesmentioning

confidence: 99%

Research Progress of Polydopamine Hydrogel in the Prevention and Treatment of Oral Diseases

Zhou¹,

Yang²,

Liu³

et al. 2023

IJN

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Oral diseases represent one of the most prevalent diseases globally and are associated with serious health and economic burdens, greatly altering the quality of life of affected individuals. Various biomaterials play important roles in the treatment of oral diseases. To some extent, the development of biomaterials has promoted progress in clinically available oral medicines. Hydrogels have unique tunable advantages that make them useful in the next generation of regenerative strategies and have been widely applied in both oral soft and hard tissues repair. However, most hydrogels lack self-adhesive properties, which may result in low repair efficacy. Polydopamine (PDA), the primary adhesive component, has attracted increasing attention in recent years. PDA-modified hydrogels exhibit reliable and suitable adherence to tissues and easily integrate into tissues to promote repair efficiency. This paper reviews the latest research progress on PDA hydrogels and elaborates on the mechanism of the reaction between PDA functional groups and hydrogels, and summarizes the biological properties and the applications of PDA hydrogels in the prevention and treatment of the field of oral diseases. It is also proposed that in future research we should simulate the complex microenvironment of the oral cavity as much as possible, coordinate and plan various biological events rationally, and realize the translation from scientific research to clinical practice.

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“…Compared with human bone marrow mesenchymal stem cells (hBMSCs), human dental pulp stem cells (hDPSCs) have stronger proliferation ability [ 1 ], and based on the same tissue origin, hDPSCs have superior feasibility for repair and regeneration of teeth and maxillofacial bone tissue. In addition, hDPSCs have the advantages of high osteo/odontogenic differentiation capacity, easy accessibility and low immunogenicity [ 1 , 2 ]. Therefore, hDPSCs have become an ideal source of stem cells for the study of tooth and bone repair and regeneration.…”

Section: Introductionmentioning

confidence: 99%

The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells

et al. 2023

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Background Human dental pulp stem cells (hDPSCs) may be the best choice for self-repair and regeneration of teeth and maxillofacial bone tissue due to their homogeneous tissue origin, high proliferation and differentiation rates, and no obvious ethical restrictions. Recently, several studies have shown that extracellular matrix (ECM) proteins can effectively regulate the proliferation and differentiation fate of mesenchymal stem cells (MSCs). However, the role of elastin microfibril interface-located protein-1 (EMILIN-1), a new ECM glycoprotein, in osteo/odontogenic differentiation of hDPSCs has not been reported. The aim of this study was to explore the effect of EMILIN-1 during osteo/odontogenic differentiation of hDPSCs. Methods hDPSCs were cultured in osteo/odontogenic induction medium. qPCR and Western blot analysis were performed to detect osteo/odonto-specific genes/proteins expression as well as the expression of EMILIN-1. After knockdown of Emilin-1 in hDPSCs with small interfering RNA and exogenous addition of recombinant human EMILIN-1 protein (rhEMILIN-1), Cell Counting Kit-8 assay, alkaline phosphatase staining, alizarin red S staining, qPCR and Western blot were performed to examine the effect of EMILIN-1 on proliferation and osteo/odontogenic differentiation of hDPSCs. Results During the osteo/odontogenic induction of hDPSCs, the expression of osteo/odonto-specific genes/proteins increased, as did EMILIN-1 protein levels. More notably, knockdown of Emilin-1 decreased hDPSCs proliferation and osteo/odontogenic differentiation, whereas exogenous addition of rhEMILIN-1 increased them. Conclusions These findings suggested that EMILIN-1 is essential for the osteo/odontogenic differentiation of hDPSCs, which may provide new insights for teeth and bone tissue regeneration.

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Application of dental pulp stem cells in oral maxillofacial tissue engineering

Cited by 27 publications

References 104 publications

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Cell Proliferation, Viability, Differentiation, and Apoptosis of Iron Oxide Labeled Stem Cells Transfected with Lipofectamine Assessed by MRI

Research Progress of Polydopamine Hydrogel in the Prevention and Treatment of Oral Diseases

The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells

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