Application of Dermal Fibroblast Cells in Cell Therapy of Wound

Nilforoushzadeh, Mohammad Ali; Ashtiani, Hamid Reza Ahmadi; Nickhah, Nahid; Fard, Maryam; Mahmoudbeyk, Mona; Jahangiri, Faeze; Ansari, Ziba Jaber; Zare, Sona

doi:10.5812/jssc.69092

Cited by 8 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cellular fibroblast pellet was extracted from a skin biopsy punched from a normal adult ear under sterile conditions at skin intervention clinics using a standard cell separation protocol. The sample was primarily cultured in high glucose DMEM with 10% FBS in a flask to achieve 70–80% confluence in each flask .…”

Section: Methodsmentioning

confidence: 99%

Non‐invasive Reflectance Spectroscopy for Normal and Cancerous Skin Cells Refractive Index Determination: An In Vitro Study

et al. 2019

View full text Add to dashboard Cite

Background and Objective: Optical reflectance spectroscopy is a non-invasive technique for optical characterization of biological samples. Any alteration in a cell from normal or carcinogenic causes will change its refractive index. The aim of this study is to develop a computerized program for extraction of a refractive index of normal and cancerous skin cell lines, including melanoma, fibroblast, and adipose cells, using visible near-infrared reflectance spectra and the Kramers-Kronig (K-K) relations. Materials and Method: A fiber optic reflectance spectrometer in visible near-infrared wavelength was used for spectrum acquisition in an in vitro study. Human skin cell lines for melanoma (A375), fibroblast, and adipose sample were cultured for optical spectroscopy. Following data acquisition, an analytical MATLAB code was developed to run the K-K relations. The program was validated for three biological samples using an Abbe refractometer. Results: The validation error (below 5%) and determination of changes in the refractive index of melanoma, normal fibroblasts, and adipose skin cells was carried out at wavelengths of 450-950 nm. The refractive index of melanoma was 1.59270 ± 0.0550 at 450 nm, the minimum amount of 1.27790 ± 0.0550 to 1.321 ± 0.0550 at 620 nm, and rose sharply to 1.44321 ± 0.0550 at 935 nm. The respective results for fibroblast and adipose tissue cells were 1.33282 ± 0.0134 and 1.28345 ± 0.0163 at 450 nm with an increasing trend to 1.30494 ± 0.0135 and 1.26716 ± 0.0163 at 935 nm. Conclusion: Refractive index characteristics show potential for cancer screening and diagnosis. The results show that optical spectroscopy is a promising, non-invasive tool for assessment of the refractive index of living biological cells in in vitro settings. Tracking changes in the refractive index allows screening of normal and abnormal cells for probable alterations in a non-invasive label-free method. Lasers Surg. Med.

show abstract

Section: Methodsmentioning

confidence: 99%

Non‐invasive Reflectance Spectroscopy for Normal and Cancerous Skin Cells Refractive Index Determination: An In Vitro Study

et al. 2019

View full text Add to dashboard Cite

show abstract

Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin‐Wound Infections

Alhayek

Khan

Schönauer

et al. 2022

Advanced Therapeutics

View full text Add to dashboard Cite

Despite the progress in surgical techniques and antibiotic prophylaxis, opportunistic wound infections with Bacillus cereus remain a public health problem. Secreted toxins are one of the main factors contributing to B. cereus pathogenicity. A promising strategy to treat such infections is to target these toxins and not the bacteria. Although the exoenzymes produced by B. cereus are thoroughly investigated, little is known about the role of B. cereus collagenases in wound infections. In this report, the collagenolytic activity of secreted collagenases (Col) is characterized in the B. cereus culture supernatant (csn) and its isolated recombinantly produced ColQ1 is characterized. The data reveals that ColQ1 causes damage on dermal collagen (COL). This results in gaps in the tissue, which might facilitate the spread of bacteria. The importance of B. cereus collagenases is also demonstrated in disease promotion using two inhibitors. Compound 2 shows high efficacy in peptidolytic, gelatinolytic, and COL degradation assays. It also preserves the fibrillar COLs in skin tissue challenged with ColQ1, as well as the viability of skin cells treated with B. cereus csn. A Galleria mellonella model highlights the significance of collagenase inhibition in vivo.

show abstract

Chemically cross‐linked poly(vinyl alcohol) electrospun fibrous mats as wound dressing materials

Díez

Homer

Leslie

et al. 2021

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Poly(vinyl alcohol) (PVA) is a synthetic biocompatible polymer that is extensively used by the medical and pharmaceutical industries due to its FDA approval for in vivo applications. Its highly hydrophilic nature makes it an ideal wound dressing material, especially in the form of nanofibrous mats. RESULTS: In this work, electrospun PVA-based scaffolds suitable for wound management were created. Chemical cross-linking with citric acid and glyoxal was employed to enhance the supports' stability in aqueous environments, and cellulose nanocrystals were added during the electrospinning process to improve the mechanical properties of the final constructs. Varying the concentrations of the cross-linking agents (0.12-1 wt% citric acid and 0.06-0.5 wt% glyoxal), allowed the control of the rate and extend of dissolution, thereby tuning the properties of the materials to the specific wound types (e.g. acute vs chronic). There was an inverse relationship between the amount of cross-linkers used and the mats' weight loss (ranging from 2% to 18%) after 6 days immersion in water. All supports sustained the growth of human fibroblasts (>85% viability), whereas there was no biofilm formation when in contact with S. aureus for 24 hours. The presence of cellulose nanocrystals did not affect cytocompatibility but improved the mechanical properties of the non-woven fibres.CONCLUSION: Tailor-made biocompatible electrospun mats showing antimicrobial behaviour were successfully created through altering the concentration of chemical cross-linkers. This flexible approach offers the potential of matching the dressing to the wound type and offering a more targeted solution to wound management.

show abstract

Application of Dermal Fibroblast Cells in Cell Therapy of Wound

Cited by 8 publications

References 28 publications

Non‐invasive Reflectance Spectroscopy for Normal and Cancerous Skin Cells Refractive Index Determination: An In Vitro Study

Non‐invasive Reflectance Spectroscopy for Normal and Cancerous Skin Cells Refractive Index Determination: An In Vitro Study

Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin‐Wound Infections

Chemically cross‐linked poly(vinyl alcohol) electrospun fibrous mats as wound dressing materials

Contact Info

Product

Resources

About