Application of Differential Electrodes in a Dielectrophoresis-Based Device for Cell Separation

Shirmohammadli, Vahideh; Manavizadeh, Negin

doi:10.1109/ted.2019.2926427

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…Shirmohammadli et al have manufactured differential sidewall gold electrodes into a microfluidic device channel and achieved 100% collection efficiency of cancer cells from blood cells. 175 By introducing sidewall electrodes, they were able to reduce the applied DC potential to 3 V and observed a significant decrease in Joule heating as a result of the reduced applied voltage. Nonspecific adherence of bioparticles on the electrodes is one of the limitations that complicate DEP-based microfluidic devices.…”

Section: Patterned Electrodesmentioning

confidence: 99%

“…Numerical studies have shown that differential sidewall electrodes not only prevent the need to apply high DC dielectrophoretic voltages but also decrease the Joule heating effect. Shirmohammadli et al have manufactured differential sidewall gold electrodes into a microfluidic device channel and achieved 100% collection efficiency of cancer cells from blood cells . By introducing sidewall electrodes, they were able to reduce the applied DC potential to 3 V and observed a significant decrease in Joule heating as a result of the reduced applied voltage.…”

Section: Categories Of Microfluidic Dep Devicesmentioning

confidence: 99%

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Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles

Kwizera

Sun

White

et al. 2021

ACS Biomater. Sci. Eng.

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Manipulation of microscale bioparticles including living cells is of great significance to the broad bioengineering and biotechnology fields. Dielectrophoresis (DEP), which is defined as the interactions between dielectric particles and the electric field, is one of the most widely used techniques for the manipulation of bioparticles including cell separation, sorting, and trapping. Bioparticles experience a DEP force if they have a different polarization from the surrounding media in an electric field that is nonuniform in terms of the intensity and/or phase of the electric field. A comprehensive literature survey shows that the DEP-based microfluidic devices for manipulating bioparticles can be categorized according to the methods of creating the nonuniformity via patterned microchannels, electrodes, and media to generate the DEP force. These methods together with the theory of DEP force generation are described in this review, to provide a summary of the methods and materials that have been used to manipulate various bioparticles for various specific biological outcomes. Further developments of DEP-based technologies include identifying materials that better integrate with electrodes than current popular materials (silicone/glass) and improving the performance of DEP manipulation of bioparticles by combining it with other methods of handling bioparticles. Collectively, DEP-based microfluidic manipulation of bioparticles holds great potential for various biomedical applications.

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Section: Patterned Electrodesmentioning

confidence: 99%

Section: Categories Of Microfluidic Dep Devicesmentioning

confidence: 99%

Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles

Kwizera

Sun

White

et al. 2021

ACS Biomater. Sci. Eng.

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“…A large body of literature focuses on the electrokinetic manipulation of breast cancer cells, with MCF‐7 being the most reported cell line [50,68–75]. For the separation of these cells from blood, DEP has been extensively explored [68,69,71–76] with a strong emphasis on eDEP [69–74,76].…”

Section: Electrokinetically‐driven Microfluidics For Cancer Cellsmentioning

confidence: 99%

A survey of electrokinetically‐driven microfluidics for cancer cells manipulation

et al. 2020

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Cancer is one of the leading causes of annual deaths worldwide, accounting for nearly 10 million deaths each year. Metastasis, the process by which cancer spreads across the patient's body, is the main cause of death in cancer patients. Because the rising trend observed in statistics of new cancer cases and cancer‐related deaths does not allow for an optimistic viewpoint on the future—in relation to this terrible disease—the scientific community has sought methods to enable early detection of cancer and prevent the apparition of metastatic tumors. One such method is known as liquid biopsy, wherein a sample is taken from a bodily fluid and analyzed for the presence of CTCs or other cancer biomarkers (e.g., growth factors). With this objective, interest is growing by year in electrokinetically‐driven microfluidics applied for the concentration, capture, filtration, transportation, and characterization of CTCs. Electrokinetic techniques—electrophoresis, dielectrophoresis, electrorotation, and electrothermal and EOF—have great potential for miniaturization and integration with electronic instrumentation for the development of point‐of‐care devices, which can become a tool for early cancer diagnostics and for the design of personalized therapeutics. In this contribution, we review the state of the art of electrokinetically‐driven microfluidics for cancer cells manipulation.

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“…The capture of CTCs in the flow allows for the concentration of CTCs and their efficient analysis. There are many methods for isolating CTCs from blood samples in a microfluidic system, such as physical separation based on the fact that CTCs are larger than other blood components [ 14 , 15 , 16 ], dielectrophoresis [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ], pinch flow [ 24 , 25 , 26 ] or ultrasonic resonances [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis-Based SERS Sensors for the Detection of Cancer Cells in Microfluidic Chips

et al. 2022

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The detection of freely circulating cancer cells (CTCs) is one of the greatest challenges of modern medical diagnostics. For several years, there has been increased attention on the use of surface-enhanced Raman spectroscopy (SERS) for the detection of CTCs. SERS is a non-destructive, accurate and precise technique, and the use of special SERS platforms even enables the amplification of weak signals from biological objects. In the current study, we demonstrate the unique arrangement of the SERS technique combined with the deposition of CTCs cells on the surface of the SERS platform via a dielectrophoretic effect. The appropriate frequencies of an alternating electric field and a selected shape of the electric field can result in the efficient deposition of CTCs on the SERS platform. The geometry of the microfluidic chip, the type of the cancer cells and the positive dielectrophoretic phenomenon resulted in the trapping of CTCs on the surface of the SERS platform. We presented results for two type of breast cancer cells, MCF-7 and MDA-MB-231, deposited from the 0.1 PBS solution. The limit of detection (LOD) is 20 cells/mL, which reflects the clinical potential and usefulness of the developed approach. We also provide a proof-of-concept for these CTCs deposited on the SERS platform from blood plasma.

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Application of Differential Electrodes in a Dielectrophoresis-Based Device for Cell Separation

Cited by 15 publications

References 24 publications

Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles

Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles

A survey of electrokinetically‐driven microfluidics for cancer cells manipulation

Dielectrophoresis-Based SERS Sensors for the Detection of Cancer Cells in Microfluidic Chips

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