Application of Direct Immunofluorescence for Skin and Mucosal Biopsies: A Practical Review

Tyler, William

doi:10.1007/978-3-030-83328-2_36

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“…Direct immunofluorescence (DIF) is an essential method in dermatology to aid in diagnosis and research. It detects immunoglobulin (Ig) deposition, complement, and fibrinogen in specific areas of the skin biopsy 5 . DIF is widely used for immunobullous diseases and vasculitis; however, literature on PPD is scant.…”

Section: Introductionmentioning

confidence: 99%

A retrospective study on the direct immunofluorescence findings in pigmented purpuric dermatosis

Ilagan

2023

J Cutan Pathol

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BackgroundPigmented purpuric dermatosis (PPD) is characterized by grouped petechiae, purpuric macules, and pigmentation in the bilateral lower extremities. It runs a chronic and relapsing course. Pathophysiology is poorly understood, but it has been proposed to be an immune‐complex disease or capillaritis. This study aimed to determine the incidence and patterns of positive direct immunofluorescence (DIF) findings in patients with clinically and histopathologically confirmed PPD. The association between DIF deposition type and clinical profile was also analyzed.MethodsPatients with a clinical and histopathologic PPD diagnosis who had undergone DIF studies at a tertiary medical center with attached dermatopathology and immunofluorescence diagnostic centers between January 2002 and December 2021 were included in this study. Data on age, sex, disease duration, comorbidities, and drug intake were collected from medical records.ResultsThere were 65 patients who satisfied the inclusion criteria. Among them, 58 (89%) had at least one positive finding and 53 (82%) were vascular deposition of immunoglobulin (Ig), complement, or fibrinogen. The most common vascular deposition was fibrinogen (71%) followed by C3 (62%), IgM (18%), IgA (6%), and IgG (3%). Fibrinogen deposition was associated with hypertension (p < 0.03). There was no association between vascular DIF deposition of IgG, IgA, and C3, with age, sex, comorbidities, disease duration, and drug history.ConclusionThe most common DIF findings in PPD were vascular deposition of fibrinogen and C3, with or without Ig presence. DIF findings supported a vascular origin in PPD but not an immune complex‐mediated disease. Hypertension was associated with fibrinogen deposition and may play a role in its pathophysiology.

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Section: Introductionmentioning

confidence: 99%

A retrospective study on the direct immunofluorescence findings in pigmented purpuric dermatosis

Ilagan

2023

J Cutan Pathol

View full text Add to dashboard Cite

show abstract

Application of Direct Immunofluorescence for Skin and Mucosal Biopsies: A Practical Review

Cited by 1 publication

References 63 publications

A retrospective study on the direct immunofluorescence findings in pigmented purpuric dermatosis

A retrospective study on the direct immunofluorescence findings in pigmented purpuric dermatosis

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