Application of Electron Microscopy in Viral Diagnosis

Kjeldsberg, Elisabeth́

doi:10.1016/s0344-0338(80)80179-8

Cited by 14 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The benefits and limitations, e.g. the 'open view' and rapidity of the individual EM diagnosis on the one hand and the need for high particle concentrations resulting in relatively low sensitivity of diagnostic EM on the other hand have been discussed in many reports (Gardner, 1977;Hsiung et al, 1979;Almeida, 1979;Kjeldsberg, 1980;Field, 1982;Almeida, 1983;Flewett, 1985; and Martin, 1988b;Miller and Howell, 1997;Gelderblom et al, 1991;Mahony and Chernesky, 1991;Landry and Hsiung, 1994;Chrystie, 1996;Madeley, 1995;Biel and Gelderblom, 1997). Today, the benefits of diagnostic EM can range from null to remarkably high, depending on the diagnostic problem itself.…”

Section: What Are the Specific Benefits Of Em In Viral Diagnosis Compmentioning

confidence: 99%

Diagnostic electron microscopy is still a timely and rewarding method

Biel

Gelderblom

1999

Journal of Clinical Virology

116

View full text Add to dashboard Cite

In the history of diagnostic EM in virology, a change in use has been seen. Starting in the 1990s and coincident with the broad introduction of 'modern' diagnostic techniques, the number of EM diagnostic labs has decreased considerably--in spite of the obvious advantages of this technique. To guarantee the continuing performance of diagnostic EM in the future. EQA runs have to be performed as with other techniques in the diagnostic armament. The growing number of participants and participating countries indicates an interest in as well as a need for this program.

show abstract

Section: What Are the Specific Benefits Of Em In Viral Diagnosis Compmentioning

confidence: 99%

Diagnostic electron microscopy is still a timely and rewarding method

Biel

Gelderblom

1999

Journal of Clinical Virology

116

View full text Add to dashboard Cite

show abstract

“…In 1072, Kapikian et al then visualized, using immune electron microscopy (EM), 27-nm viral particles that were responsible for an outbreak of acute nonbacterial gastroenteritis that occurred among school children in Norwalk, Ohio. Viral diarrhea is now well recognized, and numerous reviews and overviews of ultrastructural findings have been published on the subject (Kjeldsberg, 1980;Wolf and Schreiber, 1982;Brandt et al, 1984;Cukor and Blacklow, 1984;Yong and Peter, 1984). Viral diarrhea is now well recognized, and numerous reviews and overviews of ultrastructural findings have been published on the subject (Kjeldsberg, 1980;Wolf and Schreiber, 1982;Brandt et al, 1984;Cukor and Blacklow, 1984;Yong and Peter, 1984).…”

Section: Introductionmentioning

confidence: 99%

An Eight-Year Study of the Viral Agents of Acute Gastroenteritis in Humans: Ultrastructural Observations and Seasonal Distribution with A Major Emphasis on Coronavirus-like Particles

Borduin

et al. 1987

Coronaviruses

View full text Add to dashboard Cite

During an 8-yr period, 862 stool specimens from patients with gastroenteritis were examined by electron microscopy after negative staining with 2% phosphotungstic acid (pH 6.5). Fortyone percent of the specimens submitted over an 8-yr period were determined to be positive for virus or viruslike particles belonging to one or more of seven morphologically distinct viral groups. Coronavirus-like particles (CVLPs) were present in 69.8% of the positive stool specimens. Membranous profiles containing "complement-type" holes (10 nm in diameter) were identified in some preparations containing CVLPs. The second most prevalent viral agent found in stool specimens was the rotavirus (17% of all positive stools). The incidence of other viruses identified in the survey were as follows: adenovirus 4.5%, picorna/parvovirus agents 2.9%, Norwalk-like agent 2.9%, astrovirus 1.9%, and calicivirus 0.5%. Unclassified small round viruses (-25-30 nm in diameter) represented 0.5%. It was also determined that there was a seasonal distribution in excretion of all viruses except for CVLPs. A greater number of viruses were identified in the cooler, drier months of the year.

show abstract

“…The electron microscope has become an invaluable tool in the diagnosis of viral infections (Kjeldsberg, 1980;Doane, 1980;Almeida, 1980). This is especially true in the examination of faeces where several non-cultivable viruses can be present.…”

Section: Rapid Detection Of Human Viruses In Faeces By a Simple And Rmentioning

confidence: 99%

“…However, Paver et al (1973) have reported that parvovirus-like particles are hardly distinguished from other small cellular debris examined by direct EM unless aggregated by immune serum or collected inside a membrane. Also, small icosahedral viruses may be distinguished from each other on close examination (Kjeldsberg, 1977;Madeley, 1979), but such viruses, or viruses with uncharacterized morphology, are more easily detected when aggregated, especially when the viral concentration is low (Kjeldsberg, 1980;Almeida, 1980). Accordingly, the use of the human gamma-globulinsin-agar method would appear more profitable since it allows the formation of a wide range of viral immune complexes because it contains antibodies to a variety of viruses.…”

Section: Rapid Detection Of Human Viruses In Faeces By a Simple And Rmentioning

confidence: 99%