Application of Electron Paramagnetic Resonance Spectroscopy to Comparative Examination of Different Groups of Free Radicals in Thermal Injuries Treated with Propolis and Silver Sulphadiazine

Olczyk, Paweł; Ramos, Paweł; Bernaś, Marcin; Komosińska-Vassev, Katarzyna; Stojko, Jerzy; Pilawa, Barbara

doi:10.1155/2013/851940

Cited by 9 publications

(10 citation statements)

References 17 publications

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“…Furthermore, polyphenols increase the absorption of ascorbate, stabilize its particles, reduce its oxygenated form and decrease its metabolism [43]. Because of its high content of various polyphenol compounds, propolis displays powerful antioxidative activity, which has been confirmed in many studies, both in vitro [52,53,54,55] and in vivo [56].…”

Section: The Biological Properties Of Polyphenols Including Theirmentioning

confidence: 99%

Structure and Antioxidant Activity of Polyphenols Derived from Propolis

Kurek-Górecka¹,

et al. 2013

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PurposePrimary congenital glaucoma (PCG), a severe form of glaucoma that presents early in life, is an autosomal recessive eye disorder that results from defects in anterior eye segment. Null mutations in LTBP2 were reported in patients with PCG in Pakistani and Iranian families. This study was aimed to identify the mutation profile of the LTBP2 gene in north Indian patients with PCG.MethodsAfter ethical clearance, 54 unrelated patients with PCG who were either negative or heterozygous for MYOC, CYP1B1, and FOXC1 mutations and 50 ethnically matched non-glaucomatous controls were recruited for the study. PCG diagnosis was established by the presence of buphthalmos in at least one affected eye and associated high intraocular pressure before the age of 3 years. LTBP2 was screened in genomic blood DNA for mutations, with PCR and direct sequencing of PCR amplified fragments.ResultsWe observed one intronic single nucleotide polymorphism (rs3742793) between exons 6 and 7 in the LTBP2 gene in 18 patients with PCG. This nucleotide change resulted in cytosine (C) being replaced by guanosine (G) at position g.75070493. No pathogenic variants were identified in the LTBP2 gene in our cohort of patients.ConclusionsLTBP2 gene mutations are not involved in the pathogenesis of primary congenital glaucoma in our patients. Thus, it is important to screen other glaucoma-associated loci and genes for involvement in congenital glaucoma in cases that are either negative or heterozygous for MYOC, CYP1B1, and FOXC1 mutations to have better insight into the disease pathogenesis.

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Section: The Biological Properties Of Polyphenols Including Theirmentioning

confidence: 99%

Structure and Antioxidant Activity of Polyphenols Derived from Propolis

Kurek-Górecka¹,

et al. 2013

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“…The following parameters of EPR spectra: -factor,amplitude, -integral intensity, and linewidth (Δ pp ), were determined as was described elsewhere [6][7][8][10][11][12].…”

Section: Eprmentioning

confidence: 99%

“…The integral intensities of the EPR lines of examined steroids ( ) and ultramarine ( ) were calculated by double integration of the first-derivative EPR spectra. Free radical concentrations ( ) in the studied samples were determined based on the proportional relationship with integral intensity of the EPR lines [6][7][8][10][11][12]. Sample integral intensities in EPR spectra were examined and compared with an ultramarine reference.…”

Section: Eprmentioning

confidence: 99%

“…Electron paramagnetic resonance (EPR) spectroscopy is a known electromagnetic technique used for recording paramagnetic centers, such as free radicals [1][2][3][4][5]. Thus, it has application in different research fields like medicine and pharmacy, for example, to characterize the mechanism of pharmacological action of drugs or to estimate the effect of radiation on drug delivery system and also on its stability in solid state and in aqueous solutions, respectively [1,[6][7][8][9][10][11][12][13]. Recently, the current literature review proves various applications of EPR spectroscopy in pharmacy, for example, to examine the stability of UV irradiated and thermally sterilized drugs, especially antibiotics [6,13] and glucocorticosteroids [7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of UV Irradiation and Temperature on Free Radical Properties in Dehydrocholic and Ursodeoxycholic Acids: An EPR Study

Dołowy

Ramos

Pilawa

2014

International Journal of Photoenergy

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The effect of UV irradiation and temperature on the formation and properties of free radicals in two pharmaceutical important bile acids, such as dehydrocholic (DH) and ursodeoxycholic acids (UDC), was examined. Electron paramagnetic resonance (EPR) spectroscopy was applied to determine the paramagnetic character of UV irradiated and thermally sterilized drugs. Thermal and UV irradiation sterilizations of both compounds were carried out at different conditions according to pharmaceutical norms. The performed EPR measurements of UV irradiated and thermally sterilized DH and UDC samples proved the existence of the complex free radical systems in examined bile acids. Significant influence of UV irradiation in comparison with applied thermal sterilization on free radical concentrations in DH and UDC samples was observed. The results pointed out that thermal method is most suitable for bile acid sterilization. Therefore, this kind of sterilization should be applied in practice.

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“…Free radicals have unpaired electrons that cause major negative biochemical reactions in living organisms . Oxygen free radicals are highly reactive and are believed to be involved in the pathogenesis of many diseases.…”

Section: What Is Known and Objectivementioning

confidence: 99%