Application of Ethanol Extracts From Alnus sibirica Fisch. ex Turcz in Hair Growth Promotion

Abstract

Alnus sibirica Fisch. ex Turcz (ASFT), belonging to the family of Betulaceae, grows naturally in Asia, Europe, and America. The aims of this study are determining the efficacy of various biomarkers related to hair loss, evaluated by extracting the branch with 60% alcohol, and purely separating diarylheptanoid oregonin, an indicator and active substance, from 60% alcohol extract of the tree. To determine the preventive effects on hair loss, we investigated the anti-oxidative and anti-apoptotic effects on hydrogen peroxide-induced cytotoxicity on human hair dermal papilla cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Western blotting analysis for proving of apoptosis-related marker alteration, respectively. Moreover, we examined the ameliorative effects of 60% alcohol extract of the tree and oregonin against changes of oxidative stress-induced cytokine and testosterone-induced dihydrotestosterone production as crucial pathways of the hair loss mechanism. These results suggest that 60% alcohol extract of the tree and oregonin were available as novel natural materials for maintaining hair health in mammals.

Ulmus davidiana var. japonica Extracts Suppress Lipopolysaccharide-Induced Apoptosis Through Intracellular Calcium Modulation in U937 Macrophages

“…In many similar studies, an optimal extraction method was developed to obtain large quantities of effective substances from the same amount of natural raw materials while considering cost, space, facilities, and infrastructure (Liu et al, 2017;Hu et al, 2018;Lu et al, 2019;Kumar et al, 2020;Wang et al, 2020;Xu et al, 2020c;Yang et al, 2020;Ha et al, 2021;Huang et al, 2021;Zheng et al, 2021). Accordingly, the most commonly used extraction methods in academia and industry include hot water extraction, ethanol extraction, ultrasonic extraction, pressurized extraction, and supercritical extraction (Si et al, 2011;Chen et al, 2016;Hu et al, 2017;Xie et al, 2018Xie et al, , 2019Yang et al, 2019;Xu et al, 2020aWang et al, 2021a;Liu et al, 2021aLiu et al, , 2021bLiu et al, , 2021c.…”

Connarus semidecandrus Jack Exerts Anti-Alopecia Effects by Targeting 5α-Reductase Activity and an Intrinsic Apoptotic Pathway

“…Numerous efforts to find anti-alopecia plant extracts have previously been conducted [51][52][53]. Here, we found that Cs-EE can alleviate 5α reductase activity, which inhibited the protein expression of AR.…”

Regulation of Cytokines and Dihydrotestosterone Production in Human Hair Follicle Papilla Cells by Supercritical Extraction-Residues Extract of Ulmus davidiana

“…Nevertheless, the usages of these m life-care products, as opposed to medicinal drugs, specifically need to target su tions. Second, results may depend on the extraction methods of included raw possible unpublished reports had different characteristics within certain facilit ample, given that the extracts were in a complex, multifaceted condition for In previous reports, we determined cell viabilities in HFDPCs and other cells after challenges of oxidative stress or immunotoxic agents such as hydrogen peroxide [38] or lipopolysaccharides [39], respectively. Moreover, these effects were defined as multifunctional regulation through anti-oxidative, anti-inflammatory and/or anti-microbial pathways [39,40].…”