Application of High Temperature High Resolution Gas Chromatography to Crude Extracts of Propolis

Pereira, Alberto dos Santos; Pinto, Ângelo C.; Cardoso, Jarí N.; Neto, Francisco Radler de Aquino; Ramos, Mônica Freiman de Souza; Dellamora-Ortiz, Gisela Maria; Santos, Elisabete Pereira dos

doi:10.1002/(sici)1521-4168(19980701)21:7<396::aid-jhrc396>3.0.co;2-o

Cited by 18 publications

(4 citation statements)

References 23 publications

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“…The conditions utilized resulted in chromatograms displaying peaks in a run time of 20 min, with retention time of 4.9 min and 16.5 min for p-coumaric acid and chrysin, respectively (Figure 1). This relatively short run time was considered as good, in view of other proposed methods that displayed longer time of analysis (Bankova, Popov, Marekov, 1982;Christov, Bankova, 1992;Pereira et al, 1998;Maciejewicz et al, 2001;Bruschi et al, 2003;Nunes et al, 2012;Rosseto et al, 2017). Moreover, the selectivity of both methods was observed with no interference of solvents.…”

Section: Specificitymentioning

confidence: 80%

“…During the analysis of complex biologic matrices like PRP extract samples, the methodology type and its use determine the parameters to be evaluated (Bruschi et al, 2003). Moreover, regarding the high number of compounds and chemical complexity of PRP, the peak resolution of markers could be considered (Bankova, Popov, Marekov, 1982;Christov, Bankova, 1992;Pereira et al, 1998;Maciejewicz et al, 2001;Bruschi et al, 2003;Nunes et al, 2012;Rosseto et al, 2017).…”

Section: Robustnessmentioning

confidence: 99%

“…The liquid, thin layer and gas chromatography and/ or mass spectrometry have been proposed for analysis of PRP extracts (Bankova, Popov, Marekov, 1982;Christov, Bankova, 1992;Pereira et al, 1998;Maciejewicz et al, 2001;Bruschi et al, 2003;Nunes et al, 2012;Rosseto et al, 2017). Most of these analytical procedures showed to be composed of a long time of analysis, with complex steps, and also dependent of several and expensive analytical standards and reagents.…”

Section: Introductionmentioning

confidence: 99%

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Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts

Vecchi

Santos

Bruschi

2023

Braz. J. Pharm. Sci.

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Brazilian green propolis has been widely used in food and pharmaceutical products due to its valuable source of phenolic compounds and versatile biological activities. The development and validation of analytical methods are extremely useful for the characterization and quality control of products containing propolis. Therefore, the aim of this study was to optimize, validate and investigate the applicability of a reversed-phase HPLC method for analysis of different types of Brazilian green propolis extracts (glycolic and ethanolic). The method showed to be selective for the propolis phenolic markers. The analysis of variance and residues demonstrated that the method had significant linear regression, without lack of fit. It was also a precise, accurate and robust method, which was of utmost importance to analyze both glycolic and ethanolic extracts and at different concentrations. Moreover, as these products can display most complex matrices to analyze, a valid HPLC method can also prove to be specific and versatile.

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Section: Specificitymentioning

confidence: 80%

Section: Robustnessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts

Vecchi

Santos

Bruschi

2023

Braz. J. Pharm. Sci.

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“…Porém, a análise direta de amostras por HRGC-MS pode levar à perda de componentes termolábeis. É uma técnica bastante utilizada como ferramenta para classificação fitoquímica de amostras (PEREIRA et al, 1998).…”

Section: íNdices Analíticosunclassified

Obtenção de cera de cana-de-açucar a partir de subproduto da industria sucro-alcooleira

Vieira¹

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pelas sugestões, pelo inestimável auxílio na parte experimental e pela amizade; Aos professores Dra. Marisa A. B. Regitano-D´Arce e Dr. Gil Eduardo Serra, à Dra. Caroline Joy Steel e Dra. Nara Lúcia Facioli pelas correções e sugestões que permitiram o aprimoramento deste trabalho e pela participação na banca; Aos técnicos, estagiários e colegas do Laboratório de Óleos e Gorduras-DTA-FEA, em especial à Camila, Sylaine, Maria, Juliana, Marinalda e Neiva, pela convivência agradável e incentivo. À Victória, pelas informações e colaboração nas análises de DSC e pela amizade;

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Further lipophilic flavonols in Vellozia graminifolia (Velloziaceae) by high temperature gas chromatography: quick detection of new compounds

Branco

Pereira

Cardoso

et al. 2001

Phytochemical Analysis

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Further lipophilic flavonols present in Vellozia graminifolia have been determined by high temperature high resolution gas chromatography (HTHRGC) and by HTHRGC coupled to mass spectrometry (MS). These methods resulted in the detection, isolation and characterisation of a monoisoprenylated flavonol 3,5,4'-trimethoxy-3'-hydroxy-6,7-(2"-isopropenyldihydrofurano)flavone from the ethyl acetate extract of the whole plant. The structural elucidation was accomplished using spectral data, including two-dimensional NMR, and on chemical transformations. Both HTHRGC and HTHRGC-MS were shown to be alternative and extremely valuable methods for the quick screening of flavonoid aglycones and other chemical metabolites of the Velloziaceae.

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Application of High Temperature High Resolution Gas Chromatography to Crude Extracts of Propolis

Cited by 18 publications

References 23 publications

Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts

Applicability of an HPLC method for analysis of alcoholic and glycolic Brazilian green-propolis extracts

Obtenção de cera de cana-de-açucar a partir de subproduto da industria sucro-alcooleira

Further lipophilic flavonols in Vellozia graminifolia (Velloziaceae) by high temperature gas chromatography: quick detection of new compounds

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