“…Five major flavones were quickly isolated by HSCCC from a methanol extract of the endemic species N. ramosissima, and four of them showed relaxation activity. Besides, some 61 compounds were detected in both N. ramosissima polar extracts by UHPLC-MS. Of those, four were coumarins (peaks 2, 3, 6 and 18), 15 flavanones (peaks 4, 7, 9, 11, 15, 17, 21, 22, 32, 38, 46-49 and 56), 12 flavones (peaks 12, 13, 19, 23, 30, 39, 40-44 and 50), 3 phenolic acids (peaks 5,8 and 10) and 22 oxylipins/fatty acids (peaks 1,14,16,20,24,29,31,[33][34][35][36][37], 51-55, 57-61). Compound 2 presented higher relaxation effects than N. ramosissima extract.…”