Application of hyperosmotic agent to determine gastric cancer with optical coherence tomography ex vivo in mice

Abstract. Previous studies have demonstrated the ultrasound-induced skin optical clearing enhancement with topical application of 60% glycerol ͑G͒ on in vitro porcine skin and in vivo human skin. Our purpose was to find the relation between the effect of optical skin clearing and different concentrations of glycerol and to find more effective ultrasound-glycerol combinations on optical skin clearing. The enhancement effect of ultrasound ͓Sonophoresis ͑SP͒ delivery͔ in combination with 40% G, 60% G, and 80% G on in vitro human skin optical clearing was investigated. Light imaging depths of skin were measured using optical coherence tomography. Different concentrations of glycerol and ultrasound with a frequency of 1 MHz and an intensity of 0.5 W / cm 2 was simultaneously applied for 15 min. The results show that with the increase of concentration of glycerol, the optical clearing of skin is much improved. Optical clearing capability of glycerol was more enhanced with simultaneous application of ultrasound compared with glycerol alone. The attenuation coefficients of skin tissues after application of 40% G/SP, 60% G/SP, and 80% G/SP decreased approximately 11.8%, 18.5%, and 20.0% at 15 min compared with 40% G, 60% G, and 80% G alone, respectively. The greatest decrease in attenuation coefficients at 60 min was approximately 52.3% and 63.4% for 80% G ͑without ultrasound͒ and 80% G/SP ͑with ultrasound͒, respectively, which are 2.1-fold and 2.6-fold to that in the 40% G.

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“…Also, biological tissue is a turbid nonuniform tissue with some variations in the refractive indexes through its layers. 54 …”

Section: Without and With Ultrasoundmentioning

confidence: 99%

In vitro study of ultrasound and different-concentration glycerol–induced changes in human skin optical attenuation assessed with optical coherence tomography

et al. 2010

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“…For instance, OCT is already used in gastroenterology to detect malignant lesions in the gastrointestinal tract as well as possible responses to the resulting cancer therapy [9-11]. Moreover, application possibilities of OCT regarding breast cancer have been investigated in gynecology [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Different Nonmelanoma Skin Cancer Types Using OCT

Batz

Wahrlich

Alawi

et al. 2018

Skin Pharmacol Physiol

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Background: Early detection of various types of nonmelanoma skin cancer has been a challenge in dermatology. Noninvasive examination procedures such as optical coherence tomography (OCT) play an increasingly important role, besides the established gold standard of histological tissue sample analysis. OCT is a noninvasive, cross-sectional, real-time technique that allows conclusions to be drawn with regard to the presence of pathologies. Objective: The objective of this study was to investigate whether it is possible to distinguish between different types of nonmelanoma skin cancer using OCT or not. Methods: A study population of a total of 25 cases, comprising 5 cases, each, of 5 tumor entities (i.e., basal cell carcinoma, superficial basal cell carcinoma, actinic keratosis, squamous cell carcinoma, and Bowen disease) was examined. Relevant lesions were scanned both centrally and peripherally in the multislice mode. All OCT images were blinded, randomized, analyzed, and evaluated by 2 clinicians experienced in OCT. Results: This study demonstrated that it is possible to determine correlations between various types of tumors and recurring tumor characteristics. Conclusion: This study showed that it is possible to distinguish between the different nonmelanoma skin cancers by using OCT, but further prospective studies have to be conducted to validate the sensitivity and specificity of the criteria.

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“…Due to the hyperosmotic agent diffusion in tissues, the light scattering coefficient decreased in the specific depth region, which can be clearly reflected from the OCTSS graphs [62]. In this study, we applied the same method to monitor and compute the differences in permeability coefficient of glycerol diffusion in the human normal breast tissues and breast cancer tissues.…”

Section: Methodsmentioning

confidence: 94%

Quantification of glycerol diffusion in human normal and cancer breast tissuesin vitrowith optical coherence tomography

et al. 2010

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Optical coherence tomography (OCT) holds great promise as a routine research tool for analysis of identifying the boundaries between normal and diseased breast tissue in vitro and in vivo. However, despite the depth penetration afforded by this imaging modality, light attenuation in tissues imposes limitations. Here we studied the optical clearing effect of glycerol in human cancer and normal breast tissues with OCT for functional imaging to monitor. Depth- and time-resolved profiles for OCT signal enhancement were presented. The results show that the OCT imaging depth and imaging contrast of breast tissues have been improved after application of 60% glycerol in the 2-D OCT images. The OCT slope signals of breast tissues decreased as glycerol diffusion into tissues, therefore, the water and intercellular fluids were drawn out from tissues. Then the reverse process due to water was drawn back into the cells as a result of its affinity for water. The permeability coefficient of 60% glycerol was (3.14±0.07)×10-5 cm/s in breast cancer tissues, and (0.89±0.02)×10-5 cm/s in normal breast tissues, respectively. The permeability coefficient of glycerol in cancer tissues was 3.54-fold than that in normal tissues. These results demonstrate that the optical clearing of normal and cancer breast tissues are improved after application of glycerol.

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Application of hyperosmotic agent to determine gastric cancer with optical coherence tomography ex vivo in mice

Cited by 23 publications

References 23 publications

In vitro study of ultrasound and different-concentration glycerol–induced changes in human skin optical attenuation assessed with optical coherence tomography

In vitro study of ultrasound and different-concentration glycerol–induced changes in human skin optical attenuation assessed with optical coherence tomography

Differentiation of Different Nonmelanoma Skin Cancer Types Using OCT

Quantification of glycerol diffusion in human normal and cancer breast tissuesin vitrowith optical coherence tomography

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