Application of image analysis technique for the determination of thiophanate methyl by thin-layer chromatography

Skowron, Monika; Zakrzewski, Robert; Ciesielski, Witold

doi:10.1080/03067319.2018.1435782

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…To the best of our knowledge, little attention has been paid to fungicide dissipation in apple leaves, a non‐edible part which has no direct relation to human health. Various methods have been developed for the determination of TM and MBC in biological samples, including spectrophotographic assays (İlktaç, Aksuner, & Henden, ; Jiao et al, ), capillary electrophoresis (Picó, Rodrı́Guez, & Mañes, ), Raman imaging (Li, Sun, Pu, & Jayas, ), mass spectrometry (Yang et al, ), thin‐layer chromatography coupled with image analysis (Skowron, Zakrzewski, & Ciesielski, ), high‐performance liquid chromatography (Akkbik & Hazer, ; Sandahl, Mathiasson, & Jönsson, ; Singh, Foster, & Khan, ; Veneziano et al, ; Yu, Sun, Wang, He, & Feng, ; Zhang et al, ) and liquid chromatography–mass spectrometry (Chen et al, ; Chen et al, ; Chen, Cao, & Liu, ; Dong, Yang, Pang, & Hu, ; Dreassi et al, ; Economou, Botitsi, Antoniou, & Tsipi, ; Fernández‐Alba, Tejedor, Agüera, Contreras, & Garrido, ; Ferreira et al, ; Ferreira et al, ; Golge & Kabak, ; Morales, Ruiz, Oliva, & Barba, ; Qin et al, ; Wang & Leung, ; Wu, Chen, Li, & Fan, ; Zhang, Mei, Wang, Zhang, & Zhu, ). Among these methods, the Quick Easy Cheap Effective Rugged Safe (QuEChERS) method followed by UPLC–MS/MS detection has attracted more attention because of its satisfactory sensitivity, good selectivity and short analysis time (Bruzzoniti et al, ; Lehotay, ; Wilkowska & Biziuk, ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the dissipation kinetics of thiophanate‐methyl and its metabolite carbendazim in apple leaves using a modified QuEChERS–UPLC–MS/MS method

Wang

Lian

Dong

et al. 2018

Biomedical Chromatography

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As one of the main fungicides for the apple leaf disease control, thiophanate-methyl (TM) mainly exerts its fungicidal activity in the form of its metabolite carbendazim (MBC), whose dissipation kinetics is very distinct from that of its parent but has been paid little attention. The aim of this work was to investigate the dissipation kinetics of TM and its active metabolite MBC in apple leaves using a modified QuEChERS-UPLC-MS/MS method. The results showed that TM and MBC could be quickly extracted by this modified QuEChERS procedure with recoveries of 81.7-96.5%. The method linearity was in the range of 0.01-50.0 mg kg −1 with the quantification limit of 0.01 mg kg −1 . Then this method was applied to the analysis of fungicide dissipation kinetics in apple leaves. The results showed that the dissipation kinetics of TM for the test in 3 months can be described by a first-order kinetics model with a DT 50 (dissipation half-life) range of 5.23-6.03 days and the kinetics for MBC can be described by a firstorder absorption-dissipation model with the T max (time needed to reach peak concentration) range of 4.78-7.09 days. These models can scientifically describe the behavior of TM and MBC in apple leaves, which provides necessary data for scientific application.

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Section: Introductionmentioning

confidence: 99%

Analysis of the dissipation kinetics of thiophanate‐methyl and its metabolite carbendazim in apple leaves using a modified QuEChERS–UPLC–MS/MS method

Wang

Lian

Dong

et al. 2018

Biomedical Chromatography

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“…The basis of such analysis is the conversion of spots to the peak chromatogram and its analysis using some commercially available software (TLSee-Alfatech S.p.A., Genova, Italy; JustTLC-Sweday, Lund, Sweden) or freeware license (ImageJ-National Institutes of Health, Bethesda, MD, USA). The image analysis technique, although not often used, is a very good alternative to densitometry [4][5][6][7]. Previously conducted researches confirm the compliance of the results obtained with both techniques [8].…”

Section: Introductionmentioning

confidence: 80%

Application of the TLC image analysis technique for the simultaneous quantitative determination of L-proline and L-lysine in dietary supplement

Skowron

Zakrzewski

Ciesielski

2021

JPC-J Planar Chromat

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A simple and sensitive thin-layer chromatography (TLC) method coupled with an image analysis technique was developed for the simultaneous quantitative determination of L-proline and L-lysine in dietary supplement with good precision and accuracy. Separation was performed on silica gel plates using ethanol‒toluene (2:3, V/V) as the mobile phase. The visualization of chromatograms was based on iodine–azide reaction; therefore, pre-chromatographic derivatization reaction of amino acids with phenyl isothiocyanate was performed. Digital images of TLC plate chromatograms were converted into peak chromatograms, and quantitative analysis was conducted using TLSee software.

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“…The image analysis (IA) technique requires only flatbed scanner ,,, or digital camera ,, and appropriate software. The scanner (digital camera) allows us to obtain an image of the TLC plate.…”

Section: Introductionmentioning

confidence: 99%

Image Analysis as a Tool for Understanding Quantitative Analysis in Chromatography

Skowron,

Smarzewska

2024

J. Chem. Educ.

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A new undergraduate thin-layer chromatography laboratory experiment devoted to quantitative determination of disulfiram in Anticol formulation is presented. The proposed activity can be a part of a chromatographic methods of analysis course as well as a general chemistry course. To carry out a quantitative analysis, an image analysis technique was used, namely, combination of a flatbed scanner and appropriate software. This simple and cheap experiment shows new possibilities of implementing quantitative TLC analysis into the student laboratory. The pedagogical goals of this experiment were evaluated through the observation of students' work in laboratory and their final reports following the exercise. The presented exercise was successfully completed by third-year analytical chemistry students. The experiment integrates laboratory manual practice with other skills such as usage of the spreadsheet (e.g., Excel) for mathematical calculations and proper reasoning based on the results.

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Application of image analysis technique for the determination of thiophanate methyl by thin-layer chromatography

Cited by 12 publications

References 17 publications

Analysis of the dissipation kinetics of thiophanate‐methyl and its metabolite carbendazim in apple leaves using a modified QuEChERS–UPLC–MS/MS method

Analysis of the dissipation kinetics of thiophanate‐methyl and its metabolite carbendazim in apple leaves using a modified QuEChERS–UPLC–MS/MS method

Application of the TLC image analysis technique for the simultaneous quantitative determination of L-proline and L-lysine in dietary supplement

Image Analysis as a Tool for Understanding Quantitative Analysis in Chromatography

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