Biobased nylon (PA56) not only has the same physical properties as nylon (PA6/PA66) but its production method is also more environmentally friendly. PA56 fabric has the advantages of moisture absorption, perspiration, high-temperature resistance, and flexibility, which have been widely studied by scientific researchers. Wool has the advantages of beauty, environmental protection, and anti-wrinkle. However, pure wool fabrics have low strength and are easy to shrink when washed, which has always been a problem. Hence, this work adopted the ring spinning method to prepare wool/PA56 blended yarn with wool content of 0, 10, 30, 50, 70, and 100 wt%. Thus, to examine the effects of different blending ratios and twists on yarn performance, PA56 was blended with wool. The results showed that findings indicate that yarn performance is influenced by both yarn twist and blending ratio. The yarn thickens and takes on more linear density as the blending ratio and yarn twist increase. As the wool ratio increases, the yarn’s breaking stress and breaking strain decrease. It is obvious that the strength and elongation at break of pure PA56 yarn are 2.09 cN/Dtex and 33.92%, respectively. When the wool content was 100 wt%, the strength and elongation at break of the blended yarn were 0.66 cN/Dtex and 21.15%, respectively. With the amount of wool blending, the yarn hairiness index’s H-value initially rises and subsequently falls. The percentage of blended wool reaches 50% at 2.14; less blending might exacerbate the yarn’s stem, resulting in neps and unevenness features. The quality of the yarn improves as the blending percentage rises. The yarn has the advantages of resource saving, biodegradability, and environmental friendliness and has a broad application prospect in the automotive interior field.