Endoscopic ultrasound (EUS) is an increasingly used imaging modality in the diagnosis and management of biliary disease. The advantage of EUS is that it allows for a precise examination of the pancreaticobiliary system due to the proximity of the endoscopic probe within the gastrointestinal tract. As EUS becomes more prevalent within gastroenterology practice, clinicians should become familiar with the endosonographic findings of the gallbladder. This chapter will review normal endosonographic gallbladder anatomy in addition to benign and malignant gallbladder pathology. The identifying endosonographic characteristics of gallbladder lesions will be discussed. We will also provide a brief review of EUS-guided tissue acquisition of the gallbladder.