Application of loop-mediated isothermal amplification (LAMP)-based technology for authentication of Catharanthus roseus (L.) G. Don

Chaudhary, Anis Ahmad; Hemant,; Mohsin, Mohd; Ahmad, Altaf

doi:10.1007/s00709-011-0293-2

Cited by 22 publications

(11 citation statements)

References 18 publications

(17 reference statements)

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“…Because of its sensitivity, specificity, efficiency, rapidity, and simplicity, the LAMP method is an ideal molecular technique for identifying certain infectious diseases 16 17 and conducting food hygiene quality control 18 19 , inspection, and quarantine 20 21 . A number of reports have demonstrated that LAMP may be useful for the detection of some plants and medicinal herbs 22 23 24 25 ; however, a standard operating procedure (SOP) for on-site molecular authentication of Chinese herbs has not yet been established. DNA barcoding is currently an effective and widely used tool that enables the accurate identification of plant species.…”

mentioning

confidence: 99%

Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence

Zhao

Shi

et al. 2016

Sci Rep

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Saffron is one of the most expensive species of Chinese herbs and has been subjected to various types of adulteration because of its high price and limited production. The present study introduces a loop-mediated isothermal amplification (LAMP) technique for the differentiation of saffron from its adulterants. This novel technique is sensitive, efficient and simple. Six specific LAMP primers were designed on the basis of the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA of Crocus sativus. All LAMP amplifications were performed successfully, and visual detection occurred within 60 min at isothermal conditions of 65 °C. The results indicated that the LAMP primers are accurate and highly specific for the discrimination of saffron from its adulterants. In particular, 10 fg of genomic DNA was determined to be the limit for template accuracy of LAMP in saffron. Thus, the proposed novel, simple, and sensitive LAMP assay is well suited for immediate on-site discrimination of herbal materials. Based on the study, a practical standard operating procedure (SOP) for utilizing the LAMP protocol for herbal authentication is provided.

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confidence: 99%

Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence

Zhao

Shi

et al. 2016

Sci Rep

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“…In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection 5 28 29 . However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA.…”

Section: Discussionmentioning

confidence: 99%

Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers

Jiang

Yuan

Liu

et al. 2015

Sci Rep

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A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.

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“…Similar method was also devoted to the identification of traditional Chinese medicine Cordyceps sinensis (dong chong xia cao) and its adulterants C. hawresii, C. ramosa, C. liangschanensis, C. militaris, and C. barnesi (Li K. et al, 2011). In another study, LAMP can be combined with RAPD amplification to identify Catharanthus roseus (Chaudhary et al, 2012). These studies demonstrated that LAMP facilitates not only identification but also screening with high specificity.…”

Section: Overview Of Lamp Analysis Methods In Herbal Medicine Identifmentioning

confidence: 96%

Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

Xiong

Liu

et al. 2016

Front. Plant Sci.

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Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.

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Application of loop-mediated isothermal amplification (LAMP)-based technology for authentication of Catharanthus roseus (L.) G. Don

Cited by 22 publications

References 18 publications

Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence

Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence

Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers

Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

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