Application of Marker Assisted Selection for Potato Virus Y Resistance in the University of Wisconsin Potato Breeding Program

Detection of molecular markers linked to Ry genes in potato germplasm for marker-assisted selection for extreme resistance to PVY in AAFC’s potato breeding program

“…In a recent report by Fulladolsa et al (2015), six out of 19 PVY resistant cultivars/clones were free of the Ry sto marker YES3-3B and the Ry adg marker RYSC3. Moreover, of the six resistant clones/ cultivars that did not carry either YES3-3B or RYSC3, two ('Brodick' and 'Teena') likely carry Ry sto and two (CHC 39-7 and CHC 40-3) are S. chacoense clones carrying Ry chc (Fulladolsa et al 2015).…”

“…In a recent report by Fulladolsa et al (2015), six out of 19 PVY resistant cultivars/clones were free of the Ry sto marker YES3-3B and the Ry adg marker RYSC3. Moreover, of the six resistant clones/ cultivars that did not carry either YES3-3B or RYSC3, two ('Brodick' and 'Teena') likely carry Ry sto and two (CHC 39-7 and CHC 40-3) are S. chacoense clones carrying Ry chc (Fulladolsa et al 2015). In this study, the Ry sto markers YES3-3A, YES3-3B, and GP122 718 were absent in F87084 as well as its descendant F07060, even though F87084 was thought to possess Ry sto based on the available pedigree (De Jong et al 2001).…”

“…A final extension at 72°C was performed for 5 min. The PCR products of YES3-3A and YES3-3B were separated on a 1.4% agarose gel (Song and Schwarzfischer 2008) containing 1× Gel Red (Biotium, Inc., Hayward, CA) and a 10% Novex TBE polyacrylamide gel (PAG) (Invitrogen, Carlsbad, CA) (Fulladolsa et al 2015), respectively. The PAG was stained with 1× SYBR® Gold nucleic acid stain (Life Technologies, Gaithersburg, MD) and visualized on the BioSpectrum® Imaging System™ (UVP, Inc., Upland, CA), whereas the agarose gel was visualized directly on the imaging system.…”

“…andigena, S. stoloniferum, and S. chacoense, respectively, have been identified in potato germplasm and are used for breeding potato cultivars with ER against PVY (Fulladolsa et al 2015).…”

Interspecific potato hybrids as donors of durable resistance to pathogens

“…Подбор роди-тельских компонентов по генетической отдаленности обеспечивает гетерозиготность их потомства, что ведет к увеличению частоты появления гибридов с повышен-ной урожайностью (Яшина и др., 2010). Генетическое разнообразие современного сортимента и селекционных клонов картофеля оценивают с помощью ДНК-маркеров, ассоциированных с R-генами устойчивости к XBK, YBK, нематоде и фитофторозу (Milczarek et al, 2011;Gebhardt, 2013;Scharma et al, 2014;Бирюкова и др., 2015;Fulladolsa et al, 2015;Lopez et al, 2015;Ramakrishnan et al, 2015).…”