Application of membrane-active peptides for drug and gene delivery across cellular membranes

Plank, Christian; Zauner, Wolfgang; Wagner, Ernst

doi:10.1016/s0169-409x(98)00005-2

Cited by 165 publications

(114 citation statements)

References 107 publications

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“…For endosomal release, other factors besides the buffering capacity of oligoethylenimines ('proton sponge') 21 that include hydrophobic interactions with endosomal membranes 31 or the cationic nature of the polymers [32][33][34] may play an important role mimicking the membrane-disruptive properties of viruses or natural lytic agents. 35 According to the influences of the surface modification unit on cytotoxicity, we observed that an increasing number of nitrogen per coupled oligoamine led to an increased toxic effect if plain conjugates were used. This might be due to unspecific interactions of pseudodendritic surface amines with cell membranes (Figure 2).…”

Section: Discussionmentioning

confidence: 93%

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

et al. 2007

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Section: Discussionmentioning

confidence: 93%

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

et al. 2007

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“…Therapeutics agents, including antitumor drugs, act at intracellular sites and, thus, their clinical efficacy depends on efficient intracellular trafficking (Hu et al 2007;Plank et al 1998). …”

Section: Ph-dependent Membrane-lytic Activity Of Nanoparticlesmentioning

confidence: 99%

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

et al. 2015

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The encapsulation of antitumor drugs in nanosized systems with pH-sensitive behavior is a promissing approach that may enhance the success of chemotherapy in many cancers. The nanocarrier dependence on pH might trigger an efficient delivery of the encapsulated drug both in the acidic extracellular environment of tumors and, especially, in the intracellular compartments through disruption of endosomal membrane. In this context, here we reported the preparation of chitosan-based nanoparticles encapsulating methotrexate as a model drug (MTX-CS-NPs), which comprises the incorporation of an amino acid-based amphiphile with pHresponsive properties (77KS) on the ionotropic complexation process. The presence of 77KS clearly gives a pH-sensitive behavior to NPs, which allowed accelerated release of MTX with decreasing pH as well as pH-dependent membrane-lytic activity. This latter performance demonstrates the potential of these NPs to facilitate cytosolic delivery of endocytosed materials.Outstandingly, the cytotoxicity of MTX-loaded CS-NPs was higher than free drug to MCF-7 tumor cells and, to a lesser extent, to HeLa cells. Based on the overall results, MTX-CS-NPs modified with the pH-senstive surfactant 77KS could be potentially useful as a carrier system for intracellular drug delivery and, thus, a promising targeting anticancer chemotherapeutic agent.

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“…78 Most peptides designed and tested so far have either originated from earlier studies on drug delivery or have been modified from viral protein sequences. The important features of these polypeptides are thought to be their ability to bind and/or condense DNA, or to destabilize cell membranes (see review by Plank 79 ). Poly-L-lysine and its derivatives are the most widely reported polypeptides employed for gene delivery.…”

Section: Proteins and Polypeptidesmentioning

confidence: 99%

Non-viral gene delivery in skeletal muscle: a protein factory

Lu¹,

Bou‐Gharios²,

Partridge³

2003

Gene Ther

170

109

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Ever since the publication of the first reports in 1990 using skeletal muscle as a direct target for expressing foreign transgenes, an avalanche of papers has identified a variety of proteins that can be synthesized and correctly processed by skeletal muscle. The impetus to the development of such applications is not only amelioration of muscle diseases, but also a range of therapeutic applications, from immunization to delivery of therapeutic proteins, such as clotting factors and hormones. Although the most efficient way of introducing transgenes into muscle fibres has been by a variety of recombinant viral vectors, there are potential benefits in the use of non-viral vectors. In this review we assess the recent advances in construction and delivery of naked plasmid DNA to skeletal muscle and highlight the options available for further improvements to raise efficiency to therapeutic levels.

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Application of membrane-active peptides for drug and gene delivery across cellular membranes

Cited by 165 publications

References 107 publications

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

Non-viral gene delivery in skeletal muscle: a protein factory

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